Hypotonic stimulation enhances cellular uptake and cytocidal effect of anticancer drug in gastric cancer cells

• Project/Area Number: 26461988
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Shiozaki Atsushi 京都府立医科大学, 医学(系)研究科(研究院), 助教 (40568086)
• Co-Investigator(Kenkyū-buntansha): 大辻 英吾 京都府立医科大学, 医学(系)研究科(研究院), 教授 (20244600)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 胃癌 / 浸透圧 / 抗癌剤 / 消化器外科学 / 胃十二指腸外科学

Outline of Final Research Achievements

Intraperitoneal injection of paclitaxel (PTX) is the promising treatment option for peritoneal dissemination of gastric cancer (GC). This study examined whether hypotonic stimulation enhances cellular uptake and cytocidal effect of PTX in GC cells. After MKN45 cells were incubated with Oregon green 488 PTX in the iso- or hypotonic solution, cellular uptake of PTX was determined by flow cytometer. After cells were incubated with PTX for 1h under iso- or hypotonic condition, the number of viable cells was counted at 48h after stimulation in order to examine cytocidal effect of PTX. Since the fluorescence intensity of a MKN45 cell was significantly higher in the hypotonic condition, hypotonic stimulation enhanced cellular uptake of PTX. The number of viable MKN45 cells after stimulation was smaller in the hypotonic condition, suggesting that hypotonic stimulation enhanced cytocidal effect of PTX. Hypotonic stimulation enhances cellular uptake and cytocidal effect of PTX in MKN45 cells.

Research Products

• [Journal Article] Esophageal cancer stem cells are suppressed by tranilast, a TRPV2 channel inhibitor. (2017)
  • Author(s): Shiozaki A, Kudou M, Ichikawa D, Fujiwara H, Shimizu H, Ishimoto T, Arita T, Kosuga T, Konishi H, Komatsu S, Okamoto K, Marunaka Y, Otsuji E.
  • Journal Title: J Gastroenterol Volume: 印刷中 Issue: 2 Pages: 197-207
  • DOI: 10.1007/s00535-017-1338-x
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Expression and role of anion exchanger 1 in esophageal squamous cell carcinoma. (2017)
  • Author(s): Shiozaki A, Kudou M, Ichikawa D, Shimizu H, Arita T, Kosuga T, Konishi H, Komatsu S, Fujiwara H, Okamoto K, Kishimoto M, Marunaka Y, Otsuji E.
  • Journal Title: Oncotarget Volume: 8 Issue: 11 Pages: 17921-17935
  • DOI: 10.18632/oncotarget.14900
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Regulation of osmolality for cancer treatment. (2017)
  • Author(s): Shiozaki A, Ichikawa D, Kosuga T, Marunaka Y, Otsuji E.
  • Journal Title: J Physiol Sci Volume: 印刷中 Issue: 3 Pages: 353-360
  • DOI: 10.1007/s12576-017-0528-x
  • NAID: 40021176358
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Heat shock exerts anticancer effects on liver cancer via autophagic degradation of aquaporin 5. (2017)
  • Author(s): Kudou M, Shiozaki A, Kosuga T, Shimizu H, Ichikawa D, Konishi H, Morimura R, Komatsu S, Ikoma H, Fujiwara H, Okamoto K, Marunaka Y, Otsuji E.
  • Journal Title: Int J Oncol Volume: 50 Issue: 5 Pages: 1857-1867
  • DOI: 10.3892/ijo.2017.3940
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Transient Receptor Potential Melastatin 7 as an Independent Prognostic Factor in Human Esophageal Squamous Cell Carcinoma. (2017)
  • Author(s): Nakashima S, Shiozaki A, Ichikawa D, Hikami S, Kosuga T, Konishi H, Komatsu S, Fujiwara H, Okamoto K, Kishimoto M, Konishi E, Otsuji E.
  • Journal Title: Anticancer Res Volume: 37 Issue: 3 Pages: 1161-1167
  • DOI: 10.21873/anticanres.11429
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The Role of cIAP1 and XIAP in Apoptosis Induced by Tumor Necrosis Factor Alpha in Esophageal Squamous Cell Carcinoma Cells. (2017)
  • Author(s): Hikami S, Shiozaki A, Kitagawa-Juge M, Ichikawa D, Kosuga T, Konishi H, Komatsu S, Fujiwara H, Okamoto K, Otsuji E.
  • Journal Title: Dig Dis Sci Volume: 印刷中 Issue: 3 Pages: 652-659
  • DOI: 10.1007/s10620-016-4430-9
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Na+/H+ exchanger 1 has tumor suppressive activity and prognostic value in esophageal squamous cell carcinoma. (2017)
  • Author(s): Ariyoshi Y, Shiozaki A, Ichikawa D, Shimizu H, Kosuga T, Konishi H, Komatsu S, Fujiwara H, Okamoto K, Kishimoto M, Marunaka Y, Otsuji E.
  • Journal Title: Oncotarget Volume: 10 Issue: 2 Pages: 2209-2223
  • DOI: 10.18632/oncotarget.13645
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Inhibition of Regulatory Volume Decrease Enhances the Cytocidal Effect of Hypotonic Shock in Hepatocellular Carcinoma. (2016)
  • Author(s): Kudou M, Shiozaki A, Kosuga T, Ichikawa D, Konishi H, Morimura R, Komatsu S, Ikoma H, Fujiwara H, Okamoto K, Hosogi S, Nakahari T, Marunaka Y, Otsuji E.
  • Journal Title: J Cancer Volume: 7 Issue: 11 Pages: 1524-1533
  • DOI: 10.7150/jca.15181
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Successful management of a perforated interposed substernal ileocolon caused by right pleural hernia. (2016)
  • Author(s): Shiozaki A, Fujiwara H, Konishi H, Arita T, Kosuga T, Morimura R, Murayama Y, Komatsu S, Kuriu Y, Ikoma H, Nakanishi M, Ichikawa D, Okamoto K, Otsuji E.
  • Journal Title: Ann Thorac Surg Volume: 101 Issue: 1 Pages: e5-e7
  • DOI: 10.1016/j.athoracsur.2015.08.081
  • Peer Reviewed
• [Journal Article] Successful subcarinal dissection using a laparoscopic transhiatal approach for esophageal cancer with an anomalous pulmonary vein. (2016)
  • Author(s): Shiozaki A, Fujiwara H, Konishi H, Kosuga T, Komatsu S, Ichikawa D, Okamoto K,Otsuji E.
  • Journal Title: General Thoracic and Cardiovascular Surgery Volume: 64 Issue: 4 Pages: 239-242
  • DOI: 10.1007/s11748-016-0628-1
  • Peer Reviewed
• [Journal Article] Carbonic anhydrase XII as an independent prognostic factor in advanced esophageal squamous cell carcinoma. (2015)
  • Author(s): Ochi F, Shiozaki A, Ichikawa D, Fujiwara H, Nakashima S, Takemoto K, Kosuga T, Konishi H, Komatsu S, Okamoto K, Kishimoto M, Marunaka Y, Otsuji E.
  • Journal Title: Journal of Cancer Volume: 6 Issue: 10 Pages: 922-929
  • DOI: 10.7150/jca.11269
  • Peer Reviewed / Open Access
• [Journal Article] Laparoscopic transhiatal approach for resection of an adenocarcinoma in long-segment Barrett's esophagus. (2015)
  • Author(s): Shiozaki A, Fujiwara H, Konishi H, Kinoshita O, Kosuga T, Morimura R, Murayama Y, Komatsu S, Kuriu Y, Ikoma H, Nakanishi M, Ichikawa D, Okamoto K, Sakakura C, Otsuji E.
  • Journal Title: World Journal of Gastroenterology Volume: 21 Issue: 29 Pages: 8974-8980
  • DOI: 10.3748/wjg.v21.i29.8974
  • Peer Reviewed / Open Access
• [Journal Article] Evaluation of the efficacy of peritoneal lavage with distilled water in colorectal cancer surgery: in vitro and in vivo study. (2015)
  • Author(s): Takemoto K, Shiozaki A, Ichikawa D, Komatsu S, Konishi H, Nako Y, Murayama Y, Kuriu Y, Nakanishi M, Fujiwara H, Okamoto K, Sakakura C, Nakahari T, Marunaka Y, Otuji E.
  • Journal Title: J Gastroenterol. Volume: 50 Issue: 3 Pages: 287-297
  • DOI: 10.1007/s00535-014-0971-x
  • Peer Reviewed
• [Journal Article] Tumor necrosis factor-α-induced apoptosis of gastric cancer MKN28 cells: accelerated degradation of the inhibitor of apoptosis family members. (2015)
  • Author(s): Kitagawa M, Shiozaki A, Ichikawa D, Nakashima S, Kosuga T, Konishi H, Komatsu S, Fujiwara H, Okamoto K, Otsuji E.
  • Journal Title: Arch Biochem Biophys. Volume: 566 Pages: 43-48
  • DOI: 10.1016/j.abb.2014.12.003
  • Peer Reviewed
• [Journal Article] Esophagectomy with gastric tube reconstruction for patients who previously underwent free jejunal transfer. (2014)
  • Author(s): Shiozaki A, Fujiwara H, Konishi H, Ishimoto T, Morimura R, Murayama Y, Komatsu S, Kuriu Y, Ikoma H, Kubota T, Nakanishi M, Ichikawa D, Okamoto K, Sakakura C, Otsuji E.
  • Journal Title: Esophagus. Volume: 印刷中 Issue: 3 Pages: 267-271
  • DOI: 10.1007/s10388-014-0426-8
  • NAID: 40020539624
  • Peer Reviewed
• [Journal Article] Efficacy of a hypotonic treatment for peritoneal dissemination from gastric cancer cells: an in vivo evaluation. (2014)
  • Author(s): Shiozaki A, Ichikawa D, Takemoto K, Nako Y, Nakashima S, Shimizu H, Kitagawa M, Kosuga T, Konishi H, Komatsu S, Fujiwara H, Okamoto K, Marunaka Y, Otsuji E.
  • Journal Title: Biomed Res Int. Volume: 2014 Pages: 707089-707089
  • DOI: 10.1155/2014/707089
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The K-Cl cotransporter KCC3 as an independent prognostic factor in human esophageal squamous cell carcinoma. (2014)
  • Author(s): Shiozaki A, Takemoto K, Ichikawa D, Fujiwara H, Konishi H, Kosuga T, Komatsu S, Okamoto K, Kishimoto M, Marunaka Y, Otsuji E.
  • Journal Title: Biomed Res Int. Volume: 2014 Pages: 936401-936401
  • DOI: 10.1155/2014/936401
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Role of the Na+/K+/2Cl- cotransporter NKCC1 in cell cycle progression in human esophageal squamous cell carcinoma. (2014)
  • Author(s): Shiozaki A, Nako Y, Ichikawa D, Konishi H, Komatsu S, Kubota T, Fujiwara H, Okamoto K, Kishimoto M, Marunaka Y, Otsuji E.
  • Journal Title: World J Gastroenterol. Volume: 20 Issue: 22 Pages: 6844-6859
  • DOI: 10.3748/wjg.v20.i22.6844
  • Peer Reviewed / Open Access
• [Journal Article] Claudin 1 mediates tumor necrosis factor alpha-induced cell migration in human gastric cancer cells. (2014)
  • Author(s): Shiozaki A, Shimizu H, Ichikawa D, Konishi H, Komatsu S, Kubota T, Fujiwara H, Okamoto K, Iitaka D, Nakashima S, Nako Y, Liu M, Otsuji E.
  • Journal Title: World J Gastroenterol. Volume: 20 Issue: 47 Pages: 17863-17876
  • DOI: 10.3748/wjg.v20.i47.17863
  • Peer Reviewed / Open Access
• [Journal Article] Cellular physiological approach for treatment of gastric cancer. (2014)
  • Author(s): Shiozaki A, Ichikawa D, Otsuji E, Marunaka Y.
  • Journal Title: World J Gastroenterol. Volume: 20 Issue: 33 Pages: 11560-11566
  • DOI: 10.3748/wjg.v20.i33.11560
  • Peer Reviewed / Open Access
• [Journal Article] Progress in cellular physiological researches on esophageal cancer. (2014)
  • Author(s): Shiozaki A, Ichikawa D, Fujiwara H, Otsuji E, Marunaka Y.
  • Journal Title: Journal of Tumor Volume: 2 Pages: 241-245
  • Peer Reviewed / Open Access
• [Journal Article] Hand-assisted laparoscopic transhiatal esophagectomy with a systematic procedure for en bloc infracarinal lymph node dissection. (2014)
  • Author(s): Fujiwara H, Shiozaki A, Konishi H, Komatsu S, Kubota T, Ichikawa D, Okamoto K, Morimura R, Murayama Y, Kuriu Y, Ikoma H, Nakanishi M, Sakakura C, Otsuji E.
  • Journal Title: Dis Esophagus. Volume: Published online Issue: 2 Pages: 131-138
  • DOI: 10.1111/dote.12303
  • Peer Reviewed / Open Access
• [Journal Article] xCT, component of cysteine/glutamate transporter, as an independent prognostic factor in human esophageal squamous cell carcinoma. (2013)
  • Author(s): Shiozaki A, Iitaka D, Ichikawa D, Nakashima S, Fujiwara H, Okamoto K, Kubota T, Komatsu S, Kosuga T, Takeshita H, Shimizu H, Nako Y, Sasagawa H, Kishimoto M, Otsuji E.
  • Journal Title: J Gastroenterol Volume: 印刷中 Issue: 5 Pages: 853-863
  • DOI: 10.1007/s00535-013-0847-5
  • Peer Reviewed / Open Access
• [Journal Article] The expression and role of Aquaporin 5 in esophageal squamous cell carcinoma (2013)
  • Author(s): H. Shimizu, A. Shiozaki, E. Otsuji, et al
  • Journal Title: J Gastroenterol Volume: (印刷中) Issue: 4 Pages: 655-666
  • DOI: 10.1007/s00535-013-0827-9
  • Peer Reviewed
• [Journal Article] Perioperative outcomes of esophagectomy preceded by the laparoscopic transhiatal approach for esophageal cancer. (2012)
  • Author(s): Shiozaki A, Kokuba Y, Otsuji E, et al.
  • Journal Title: Diseases of the Esophagus Volume: (印刷中) Issue: 5 Pages: 470-478
  • DOI: 10.1111/j.1442-2050.2012.01439.x
  • Peer Reviewed
• [Presentation] Roles of anion exchanger 1 in esophageal squamous cell in carcinoma. (2016)
  • Author(s): Shiozaki A, Kudou M, Ichikawa D, Shimizu H, Kosuga T, Konishi H, Komatsu S, Okamoto K, Fujiwara H, Yamagishi H, Otsuji E.
  • Organizer: 40th World Congress of the International College of Surgeons
  • Place of Presentation: 国立京都国際会館（京都市）
  • Year and Date: 2016-10-23
  • Int'l Joint Research
• [Presentation] AE2, Anion exchanger 2, as an independent prognostic factor in human esophageal squamous cell carcinoma. (2016)
  • Author(s): Kobayashi T, Shiozaki A, Ichikawa D, Hikami S, Kosuga T, Konishi H, Komatsu S,Fujiwara H, Okamoto K, Kishimoto M, Otsuji E.
  • Organizer: 40th World Congress of the International College of Surgeons
  • Place of Presentation: 国立京都国際会館（京都市）
  • Year and Date: 2016-10-23
  • Int'l Joint Research
• [Presentation] The efficacy and clinical safety of additional surgical resection after endoscopic submucosal dissection for esophageal cancer. (2016)
  • Author(s): Kudo M, Shiozaki A, Fujiwara H, Konishi H, Arita T, Kosuga T, Morimura R, Murayama Y, Komatsu S, Kuriu Y, Ikoma H, Nakanishi M, Ichikawa D, Okamoto K, Otsuji E.
  • Organizer: 40th World Congress of the International College of Surgeons
  • Place of Presentation: 国立京都国際会館（京都市）
  • Year and Date: 2016-10-23
  • Int'l Joint Research
• [Presentation] Prognostic significance of the sodium iodide symporter expression in human esophageal squamous cell carcinoma. (2016)
  • Author(s): Yamazato Y, Shiozaki A, Ichikawa D, Ariyoshi Y, Iitaka D, Kosuga T, Konishi H, Fujiwara H, Okamoto K, Kishimoto M, Otsuji E.
  • Organizer: 40th World Congress of the International College of Surgeons
  • Place of Presentation: Kyoto
  • Year and Date: 2016-10-23
  • Int'l Joint Research
• [Presentation] Expression of ALDH1 and/or CD 44 in esophageal squamous cell carcinoma. (2016)
  • Author(s): Tanaka S, Shiozaki A, Fujiwara H, Konishi H, Kosuga T, Komatsu S, Ichikawa D, Okamoto K, Otsuji E.
  • Organizer: 40th World Congress of the International College of Surgeons
  • Place of Presentation: 国立京都国際会館（京都市）
  • Year and Date: 2016-10-23
  • Int'l Joint Research
• [Presentation] Transient receptor potential melastatin 7 channel as an independent prognostic factor in human esophageal squamous cell carcinoma. (2016)
  • Author(s): Nakashima S, Shiozaki A, Ichikawa D, Hikami S, Kosuga T, Konishi H, Komatsu S, Fujiwara H, Okamoto K, Yamagishi H, Otsuji E.
  • Organizer: 40th World Congress of the International College of Surgeons
  • Place of Presentation: 国立京都国際会館（京都市）
  • Year and Date: 2016-10-23
  • Int'l Joint Research
• [Presentation] Heat shock induces anticancer effects via regulation of aquaporin5. (2016)
  • Author(s): Kosuga T, Shiozaki A, Kudou M, Shimizu H, Ichikawa D, Komatsu S, Konishi H, Arita T, Morimura R, Ikoma H, Otsuji E.
  • Organizer: 40th World Congress of the International College of Surgeons
  • Place of Presentation: 国立京都国際会館（京都市）
  • Year and Date: 2016-10-23
  • Int'l Joint Research
• [Presentation] Expression and role of sodium iodide symporter in gastric cancer. (2016)
  • Author(s): Ariyoshi Y, Shiozaki A, Ichikawa D, Kosuga T, Konishi H, Komatsu S, Fujiwara H, Okamoto K, Kishimoto M, Marunaka Y, Otsuji E.
  • Organizer: 40th World Congress of the International College of Surgeons
  • Place of Presentation: 国立京都国際会館（京都市）
  • Year and Date: 2016-10-23
  • Int'l Joint Research
• [Presentation] Tumor necrosis factor-a-induced apoptosis of gastric cancer MKN28 cells: Accelerated degradation of the inhibitor od adoptosis family members. (2016)
  • Author(s): Kitagawa-Juge M, Shiozaki A, Ichikawa D, Okamoto K, Fujiwara T（toshiyoshi）, Otsuji E.
  • Organizer: 40th World Congress of the International College of Surgeons
  • Place of Presentation: 国立京都国際会館（京都市）
  • Year and Date: 2016-10-23
  • Int'l Joint Research
• [Presentation] TRPV2, a potential novel target for anti-cancer therapy against esophageal cancer stem cells. (2016)
  • Author(s): Kudou M, Shiozaki A, Ichikawa D, Shimizu H, Arita T, Kosuga T, Konishi H, Morimura R, Murayama Y, Komatsu S, Ikoma H, Kuiru Y, Nakanishi M, Fujiwara H, Okamoto K, Yamagishi H, Otsuji E.
  • Organizer: 40th World Congress of the International College of Surgeons
  • Place of Presentation: 国立京都国際会館（京都市）
  • Year and Date: 2016-10-23
  • Int'l Joint Research
• [Presentation] Function of Na+/H+ exchanger 1 and its clinical significance in esophageal squamous cell carcinoma. (2016)
  • Author(s): Ariyoshi Y, Shiozaki A, Ichikawa D, Arita T, Kosuga T, Konishi H, Morimura R, Murayama Y, Komatsu S, Ikoma H, Kuriu Y, Nakanishi M, Fujiwara H, Okamoto K, Kishimoto M, Marunaka Y, Yamagishi H, Otsuji E
  • Organizer: 40th World Congress of the International College of Surgeons
  • Place of Presentation: 国立京都国際会館（京都市）
  • Year and Date: 2016-10-23
  • Int'l Joint Research
• [Presentation] 低浸透圧刺激による胃癌細胞へのパクリタキセル取り込みおよび殺細胞増強効果． (2016)
  • Author(s): 小菅敏幸、塩﨑敦、市川大輔、工藤道弘、竹本健一、小松周平、小西博貴、有田智洋、森村玲、村山康利、岡本和真、藤原斉、大辻英吾．
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2016-10-06
• [Presentation] 食道扁平上皮癌におけるAnion Exchanger1の機能解析． (2016)
  • Author(s): 塩﨑敦、工藤道弘、市川大輔、清水浩紀、小菅敏幸、小西博貴、小松周平、中西正芳、藤原斉、岡本和真、岸本光夫、丸中良典、大辻英吾．
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2016-10-06
• [Presentation] 食道扁平上皮癌におけるNa+/H+ exchanger1(NHE1)の機能および臨床病理学的意義． (2016)
  • Author(s): 有吉要輔、塩﨑敦、市川大輔、清水浩紀、小菅敏幸、小西博貴、小松周平、中西正芳、藤原斉、岡本和真、岸本光夫、丸中良典、大辻英吾．
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2016-10-06
• [Presentation] 食道癌幹細胞におけるtransient receptor potential vanilloid 2 阻害剤、tranilastの抗腫瘍効果． (2016)
  • Author(s): 工藤道弘、塩﨑敦、市川大輔、清水浩紀、有田智洋、小菅敏幸、小西博貴、小松周平、藤原斉、岡本和真、丸中良典、大辻英吾．
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2016-10-06
• [Presentation] 食道扁平上皮癌におけるAnion exchanger 2 発現の意義． (2016)
  • Author(s): 小林利行、塩﨑敦、市川大輔、藤原斉、小西博貴、樋上翔一郎、小菅敏幸、有田智洋、小松周平、中西正芳、岡本和真、岸本光夫、大辻英吾．
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2016-10-06
• [Presentation] 食道扁平上皮癌におけるNISの発現と予後との相関． (2016)
  • Author(s): 山里有三、塩﨑敦、市川大輔、有吉要輔、飯高大介、有田智洋、小菅敏幸、小西博貴、小松周平、藤原斉、岡本和真、岸本光夫、大辻英吾．
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2016-10-06
• [Presentation] 食道扁平上皮癌細胞株を用いたTNF-α誘導性アポトーシスにおけるIAPファミリー動態の検討． (2016)
  • Author(s): 樋上翔一郎、塩﨑敦、樹下真希、市川大輔、小菅敏幸、小西博貴、小松周平、栗生宜明、生駒久視、中西正芳、藤原斉、岡本和真、大辻英吾．
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2016-10-06
• [Presentation] 食道扁平上皮癌におけるAE2発現低下は予後不良因子であり、癌細胞の転移浸潤を促進する． (2016)
  • Author(s): 樋上翔一郎、塩﨑敦、小菅敏幸、小西博貴、小松周平、市川大輔、岡本和真、藤原斉、岸本光夫、大辻英吾．
  • Organizer: 第71回日本消化器外科学会総会
  • Place of Presentation: あわぎんホール（徳島市）
  • Year and Date: 2016-07-14
• [Presentation] 食道扁平上皮癌におけるNa+/H+ exchanger1(NHE1)の機能およびその臨床的意義の検討． (2016)
  • Author(s): 有吉要輔、塩﨑敦、市川大輔、小菅敏幸、小西博貴、小松周平、藤原斉、岡本和真、岸本光夫、大辻英吾．
  • Organizer: 第71回日本消化器外科学会総会
  • Place of Presentation: あわぎんホール（徳島市）
  • Year and Date: 2016-07-14
• [Presentation] 当科における食道扁平上皮癌でのALDH1/CD44発現解析． (2016)
  • Author(s): 田中幸恵、塩﨑敦、藤原斉、小西博貴、小菅敏幸、小松周平、市川大輔、岡本和真、岸本光夫、大辻英吾．
  • Organizer: 第71回日本消化器外科学会総会
  • Place of Presentation: あわぎんホール（徳島市）
  • Year and Date: 2016-07-14
• [Presentation] 食道扁平上皮癌におけるsodium iodide symporter (NIS) の臨床病理学的意義． (2016)
  • Author(s): 山里有三、塩﨑敦、市川大輔、有吉要輔、飯高大介、小菅敏幸、藤原斉、岡本和真、岸本光夫、大辻英吾．
  • Organizer: 第71回日本消化器外科学会総会
  • Place of Presentation: あわぎんホール（徳島市）
  • Year and Date: 2016-07-14
• [Presentation] 肝細胞癌におけるregulatory volume decrease 制御による低浸透圧殺腫瘍効果の増強． (2016)
  • Author(s): 小林利行、塩﨑敦、小菅敏幸、工藤道弘、森村玲、生駒久視、市川大輔、岡本和真、丸中良典、大辻英吾．
  • Organizer: 第71回日本消化器外科学会総会
  • Place of Presentation: あわぎんホール（徳島市）
  • Year and Date: 2016-07-14
• [Presentation] 胃癌細胞株における低浸透圧細胞破壊効果とカルシウム輸送制御による殺細胞効果増強の検討． (2016)
  • Author(s): 竹本健一、塩﨑敦、市川大輔、小松周平、有田智洋、小菅敏幸、小西博貴、森村玲、村山康利、栗生宜明、生駒久視、中西正芳、藤原斉、岡本和真、丸中良典、大辻英吾.
  • Organizer: 第116回日本外科学会定期学術集会
  • Place of Presentation: 大阪国際会議場（大阪市）
  • Year and Date: 2016-04-14
• [Presentation] 食道扁平上皮癌細胞株を用いたTNF-α誘導性アポトーシスにおけるIAPファミリー動態の検討． (2016)
  • Author(s): 樋上翔一郎、塩﨑敦、市川大輔、北川真希、有田智洋、小菅敏幸、小西博貴、森村玲、村山康利、小松周平、栗生宜明、生駒久視、中西正芳、藤原斉、岡本和真、大辻英吾．
  • Organizer: 第116回日本外科学会定期学術集会
  • Place of Presentation: 大阪国際会議場（大阪市）
  • Year and Date: 2016-04-14
• [Presentation] 肝細胞癌に対する温熱刺激のアクアポリン5制御を介した抗腫瘍効果と、その機序の解明． (2016)
  • Author(s): 工藤道弘、塩﨑敦、小菅敏幸、清水浩紀、有田智洋、小西博貴、森村玲、村山康利、小松周平、栗生宜明、生駒久視、中西正芳、市川大輔、藤原斉、岡本和真、大辻英吾.
  • Organizer: 第116回日本外科学会定期学術集会
  • Place of Presentation: 大阪国際会議場（大阪市）
  • Year and Date: 2016-04-14
• [Presentation] 肝細胞癌におけるRVD制御を介した低浸透圧殺細胞増強効果． (2016)
  • Author(s): 小菅敏幸、塩﨑敦、市川大輔、小松周平、小西博貴、工藤道弘、有田智洋、森村玲、村山康利、栗生宜明、生駒久視、中西正芳、岡本和真、藤原斉、大辻英吾.
  • Organizer: 第116回日本外科学会定期学術集会
  • Place of Presentation: 大阪国際会議場（大阪市）
  • Year and Date: 2016-04-14
• [Presentation] 食道扁平上皮癌におけるALDH1およびCD44発現の検討． (2016)
  • Author(s): 田中幸恵、塩﨑敦、藤原斉、小西博貴、有田智洋、小菅敏幸、森村玲、村山康利、小松周平、栗生宜明、生駒久視、中西正芳、市川大輔、岡本和真、岸本光夫、大辻英吾.
  • Organizer: 第116回日本外科学会定期学術集会
  • Place of Presentation: 大阪国際会議場（大阪市）
  • Year and Date: 2016-04-14
• [Presentation] 食道扁平上皮癌におけるNa+/H+ exchanger1(NHE1)の機能解析および臨床的意義． (2016)
  • Author(s): 有吉要輔、塩﨑敦、市川大輔、有田智洋、小菅敏幸、小西博貴、森村玲、村山康利、小松周平、生駒久視、栗生宜明、中西正芳、藤原斉、岡本和真、岸本光夫、丸中良典、大辻英吾.
  • Organizer: 第116回日本外科学会定期学術集会
  • Place of Presentation: 大阪国際会議場（大阪市）
  • Year and Date: 2016-04-14
• [Presentation] 低浸透圧刺激による胃癌細胞へのパクリタキセル取り込み増強効果． (2015)
  • Author(s): 小菅敏幸、塩﨑敦、市川大輔、飯高大介、竹本健一、小松周平、小西博貴、岡本和真、藤原斉、有田智洋、中西正芳、村山康利、大辻英吾．
  • Organizer: 第74回 日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（名古屋市）
  • Year and Date: 2015-10-08
• [Presentation] 食道扁平上皮癌におけるK-Cl共輸送体、KCC3の機能解析． (2015)
  • Author(s): 塩﨑敦、竹本健一、市川大輔、小菅敏幸、小西博貴、小松周平、生駒久視、中西正芳、藤原斉、岡本和真、岸本光夫、丸中良典、大辻英吾．
  • Organizer: 第74回 日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（名古屋市）
  • Year and Date: 2015-10-08
• [Presentation] 胃癌細胞株に対する蒸留水細胞破壊効果の検証（in vivo and in vitro study）． (2015)
  • Author(s): 竹本健一、塩﨑敦、市川大輔、小菅敏幸、小西博貴、小松周平、栗生宜明、生駒久視、中西正芳、藤原斉、岡本和真、丸中良典、大辻英吾．
  • Organizer: 第74回 日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（名古屋市）
  • Year and Date: 2015-10-08
• [Presentation] 肝細胞癌におけるregulatory volume decrease の阻害による低浸透圧殺細胞効果の増強． (2015)
  • Author(s): 工藤道弘、塩﨑敦、小菅敏幸、市川大輔、小松周平、小西博貴、有田智洋、森村玲、生駒久視、藤原斉、岡本和真、丸中良典、大辻英吾．
  • Organizer: 第74回 日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（名古屋市）
  • Year and Date: 2015-10-08
• [Presentation] 食道扁平上皮癌におけるNA+/H+ exchanger 1(NHE1)発現の意義． (2015)
  • Author(s): 有吉要輔、塩﨑敦、市川大輔、小菅敏幸、小西博貴、小松周平、生駒久視、栗生宜明、中西正芳、藤原斉、岡本和真、岸本光夫、大辻英吾．
  • Organizer: 第74回 日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（名古屋市）
  • Year and Date: 2015-10-08
• [Presentation] 食道扁平上皮癌における the sodium iodide symporter （NIS)発現の意義． (2015)
  • Author(s): 山里有三、塩﨑敦、市川大輔、小菅敏幸、飯高大介、清水浩紀、有吉要輔、小西博貴、小松周平、藤原斉、岡本和真、岸本光夫、大辻英吾．
  • Organizer: 第74回 日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（名古屋市）
  • Year and Date: 2015-10-08
• [Presentation] 食道扁平上皮癌におけるAE1発現の意義． (2015)
  • Author(s): 小林利行、塩﨑敦、藤原斉、小西博貴、工藤道弘、清水浩紀、小菅敏幸、小松周平、市川大輔、中西正芳、岡本和真、岸本光夫、大辻英吾．
  • Organizer: 第74回 日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（名古屋市）
  • Year and Date: 2015-10-08
• [Presentation] 食道扁平上皮癌におけるAE2発現意義の検討． (2015)
  • Author(s): 樋上翔一郎、塩﨑敦、市川大輔、小菅敏幸、小西博貴、小松周平、栗生宜明、生駒久視、中西正芳、藤原斉、岡本和真、岸本光夫、大辻英吾．
  • Organizer: 第74回 日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（名古屋市）
  • Year and Date: 2015-10-08
• [Presentation] 胃癌の細胞遊走ならびにTNFαシグナル伝達におけるClaudin1の役割． (2015)
  • Author(s): 清水浩紀、塩﨑敦、市川大輔、小菅敏幸、小西博貴、村山康利、小松周平、生駒久視、栗生宜明、中西正芳、藤原斉、岡本和真、大辻英吾．
  • Organizer: 第74回 日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（名古屋市）
  • Year and Date: 2015-10-08
• [Presentation] イオン輸送体制御による新たな食道癌治療戦略． (2015)
  • Author(s): 塩﨑敦、市川大輔、藤原斉、小菅敏幸、小西博貴、森村玲、村山康利、小松周平、栗生宜明、生駒久視、中西正芳、岡本和真、阪倉長平、大辻英吾．
  • Organizer: 第13回 日本消化器外科学会大会
  • Place of Presentation: グランドプリンスホテル新高輪（東京都）
  • Year and Date: 2015-10-08
• [Presentation] 細胞生理学を応用した新たな食道癌治療戦略． (2015)
  • Author(s): 塩﨑敦、藤原斉、市川大輔、小菅敏幸、小西博貴、小松周平、岡本和真、岸本光夫、丸中良典、大辻英吾．
  • Organizer: 第69回 日本食道学会学術集会
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2015-07-02
• [Presentation] マウス胃癌腹膜播種モデルにおける低浸透圧療法の安全性と治療効果の検討． (2015)
  • Author(s): 有吉要輔、塩﨑敦、市川大輔、小菅敏幸、小西博貴、森村玲、村山康利、小松周平、栗生宜明、生駒久視、中西正芳、藤原斉、岡本和真、阪倉長平、大辻英吾．
  • Organizer: 第115回 日本外科学会定期学術集会
  • Place of Presentation: 名古屋国際会議場（名古屋市）
  • Year and Date: 2015-04-16
• [Presentation] 腹腔内遊離細胞治療を目的とした大腸癌細胞株における低浸透圧細胞破壊効果とクロライドイオン輸送制御による殺細胞効果増強の検討． (2015)
  • Author(s): 竹本健一、塩﨑敦、市川大輔、小松周平、小菅敏幸、小西博貴、森村玲、村山康利、栗生宜明、生駒久視、中西正芳、藤原斉、岡本和真、阪倉長平、丸中良典、大辻英吾．
  • Organizer: 第115回 日本外科学会定期学術集会
  • Place of Presentation: 名古屋国際会議場（名古屋市）
  • Year and Date: 2015-04-16
• [Presentation] 食道扁平上皮癌におけるCarbonic Anhydrase XII発現意義の検討． (2015)
  • Author(s): 越智史明、塩﨑敦、藤原斉、小菅敏幸、小西博貴、森村玲、村山康利、小松周平、栗生宜明、生駒久視、中西正芳、市川大輔、岡本和真、阪倉長平、岸本光夫、大辻英吾．
  • Organizer: 第115回 日本外科学会定期学術集会
  • Place of Presentation: 名古屋国際会議場（名古屋市）
  • Year and Date: 2015-04-16
• [Presentation] 食道扁平上皮癌におけるKCC3発現の意義． (2015)
  • Author(s): 樋上翔一郎、塩﨑敦、小菅敏幸、小西博貴、森村玲、村山康利、小松周平、栗生宜明、生駒久視、中西正芳、市川大輔、岡本和真、藤原斉、阪倉長平、岸本光夫、丸中良典、大辻英吾．
  • Organizer: 第115回 日本外科学会定期学術集会
  • Place of Presentation: 名古屋国際会議場（名古屋市）
  • Year and Date: 2015-04-16
• [Presentation] Role of the K-Cl cotranspoter KCC3 in cell migration in human esophageal squamous cell carcinoma. (2014)
  • Author(s): Atsushi Shiozaki
  • Organizer: The 39th biennial world congress of the international college of surgeons
  • Place of Presentation: Bali, INDONESIA
  • Year and Date: 2014-10-20 – 2014-10-23
• [Presentation] Roles of E2F5 in human esophageal squamous cell carcinoma. (2014)
  • Author(s): Shiozaki A, Ishimoto T, Fujiwara H, Konishi H, Morimura R, Murayama Y, Komatsu S, Kuriu Y, Ikoma H, Kubota T, Nakanishi M, Ichikawa D, Okamoto K, Sakakura C, Kishimoto M, Otsuji E.
  • Organizer: 14th World Congress of the International Society for Diseases of the Esophagus
  • Place of Presentation: Vancouver, CANADA
  • Year and Date: 2014-09-22 – 2014-09-24
• [Presentation] Prognostic importance of the mitosis specific marker anti-phosphohistone H3 in esophageal squamous cell carcinoma. (2014)
  • Author(s): Nakashima S, Shiozaki A, Ichikawa D, Fujiwara H, Komatsu S, Konishi H, Morimura R, Murayama Y, Kuriu Y, Ikoma H, Kubota T, Nakanishi M, Okamoto K, Sakakura C, Otsuji E.
  • Organizer: 14th World Congress of the International Society for Diseases of the Esophagus
  • Place of Presentation: Vancouver, CANADA
  • Year and Date: 2014-09-22 – 2014-09-24
• [Presentation] The functional analyses of Na(+)/K(+)/2Cl(-) cotransporter 1(NKCC1) in esophageal squamous cell carcinoma. (2014)
  • Author(s): Nako Y, Shiozaki A, Ichikawa D, Fujiwara H, Konishi H, Morimura R, Murayama Y, Komatsu S, Ikoma H, Kuriu Y, Kubota T, Nakanishi M, Okamoto K, Sakakura C, Kishimoto M, Marunaka Y, Otsuji E.
  • Organizer: 14th World Congress of the International Society for Diseases of the Esophagus
  • Place of Presentation: Vancouver, CANADA
  • Year and Date: 2014-09-22 – 2014-09-24
• [Presentation] p21 expression as an independent prognostic factor in human esophageal squamous cell carcinoma. (2014)
  • Author(s): Iitaka D, Shiozaki A, Fujiwara H, Konishi H, Morimura R, Murayama Y, Komatsu S, Kuriu Y, Ikoma H, Nakanishi M, Ichikawa D, Okamoto K, Sakakura C, Otsuji E.
  • Organizer: 14th World Congress of the International Society for Diseases of the Esophagus
  • Place of Presentation: Vancouver, CANADA
  • Year and Date: 2014-09-22 – 2014-09-24
• [Presentation] 食道類基底細胞癌6症例における免疫組織学的解析． (2014)
  • Author(s): 中島慎吾、塩﨑敦、藤原斉、市川大輔、小西博貴、小松周平、窪田健、岡本和真、阪倉長平、大辻英吾．
  • Organizer: 第69回 日本消化器外科学会総会
  • Place of Presentation: 福島県郡山市
  • Year and Date: 2014-07-16 – 2014-07-18
• [Presentation] 食道扁平上皮癌におけるE２F5発現の意義． (2014)
  • Author(s): 塩﨑敦、石本武史、市川大輔、藤原斉、小西博貴、小松周平、窪田健、岡本和真、岸本光夫、大辻英吾．
  • Organizer: 第68回 日本食道学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2014-07-03 – 2014-07-04
• [Presentation] 胃癌細胞におけるTNF-αシグナル制御因子としてのClaudin 1の役割． (2014)
  • Author(s): 塩﨑敦、清水浩紀、市川大輔、小西博貴、森村玲、村山康利、小松周平、栗生宜明、生駒久視、窪田健、中西正芳、藤原斉、岡本和真、阪倉長平、Liu Mingyao、大辻英吾．
  • Organizer: 第114回 日本外科学会定期学術集会
  • Place of Presentation: 京都
  • Year and Date: 2014-04-03 – 2014-04-05
• [Presentation] 食道扁平上皮癌におけるE2F5発現の検討． (2014)
  • Author(s): 石本武史、塩﨑敦、市川大輔、小西博貴、小松周平、窪田健、藤原斉、岡本和真、落合登志哉、岸本光夫、大辻英吾．
  • Organizer: 第114回 日本外科学会定期学術集会
  • Place of Presentation: 京都
  • Year and Date: 2014-04-03 – 2014-04-05
• [Presentation] 肺癌細胞株におけるTNFa誘導CLDN1のシグナル伝達の解明． (2014)
  • Author(s): 飯高大介、塩﨑敦、市川大輔、藤原斉、小西博貴、小松周平、窪田健、岡本和真、森村玲、村山康利、栗生宜明、生駒久視、中西正芳、阪倉長平、Liu Mingyao 大辻英吾．
  • Organizer: 第114回 日本外科学会定期学術集会
  • Place of Presentation: 京都
  • Year and Date: 2014-04-03 – 2014-04-05
• [Presentation] 消化器癌細胞に対する低浸透圧刺激による殺細胞効果と蒸留水腹腔内投与における播種性転移予防効果の検討． (2014)
  • Author(s): 名幸義仁、塩﨑敦、市川大輔、小松周平、森村玲、小西博貴、村山康利、栗生宜明、生駒久視、窪田健、中西正芳、藤原斉、岡本和真、阪倉長平、丸中良典、大辻英吾．
  • Organizer: 第114回 日本外科学会定期学術集会
  • Place of Presentation: 京都
  • Year and Date: 2014-04-03 – 2014-04-05
• [Presentation] 腹膜播種転移制御を目的とした大腸癌細胞における低浸透圧細胞破壊効果とクロライドチャネル制御の利用． (2014)
  • Author(s): 竹本健一、塩﨑敦、市川大輔、小松周平、小西博貴、森村玲、村山康利、栗生宜明、生駒久視、窪田健、中西正芳、藤原斉、岡本和真、阪倉長平、丸中良典、大辻英吾．
  • Organizer: 第114回 日本外科学会定期学術集会
  • Place of Presentation: 京都
  • Year and Date: 2014-04-03 – 2014-04-05
• [Presentation] 食道扁平上皮癌症例におけるp21発現の検討． (2014)
  • Author(s): 中島慎吾、塩﨑敦、市川大輔、藤原斉、小松周平、小西博貴、森村玲、村山康利、栗生宜明、生駒久視、窪田健、中西正芳、岡本和真、阪倉長平、大辻英吾．
  • Organizer: 第114回 日本外科学会定期学術集会
  • Place of Presentation: 京都
  • Year and Date: 2014-04-03 – 2014-04-05