| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Intraperitoneal injection of paclitaxel (PTX) is the promising treatment option for peritoneal dissemination of gastric cancer (GC). This study examined whether hypotonic stimulation enhances cellular uptake and cytocidal effect of PTX in GC cells. After MKN45 cells were incubated with Oregon green 488 PTX in the iso- or hypotonic solution, cellular uptake of PTX was determined by flow cytometer. After cells were incubated with PTX for 1h under iso- or hypotonic condition, the number of viable cells was counted at 48h after stimulation in order to examine cytocidal effect of PTX. Since the fluorescence intensity of a MKN45 cell was significantly higher in the hypotonic condition, hypotonic stimulation enhanced cellular uptake of PTX. The number of viable MKN45 cells after stimulation was smaller in the hypotonic condition, suggesting that hypotonic stimulation enhanced cytocidal effect of PTX. Hypotonic stimulation enhances cellular uptake and cytocidal effect of PTX in MKN45 cells.
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