| Project/Area Number |
26461991
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
中森 幹人 和歌山県立医科大学, 医学部, 准教授 (10322372)
山上 裕機 和歌山県立医科大学, 医学部, 教授 (20191190)
中村 公紀 和歌山県立医科大学, 医学部, 講師 (80364090)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | iPS細胞 / 樹状細胞 / 腫瘍抗原 / ウイルスベクター / がんワクチン / 人工多能性幹細胞 / 腫瘍抗原遺伝子 / アデノウイルスベクター / 細胞障害性Tリンパ球 / 遺伝子組み換えマウス |
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| Outline of Final Research Achievements |
We investigated whether genetically modified human induced pluripotent stem cell (iPSC)-derived dendritic cells (hiPSDCs) expressing carcinoembryonic antigen (CEA) could induce CEA-specific cytotoxic T cells in a human model, and whether genetically modified mouse iPSDCs (miPSDCs) expressing CEA would show an actual antitumor effect using a CEA transgenic mouse model. We differentiated hiPSDCs from iPSCs of three healthy donors and transduced the CEA cDNA into hiPSDCs. The cytotoxic T cells induced by hiPSDCs-CEA exhibited CEA-specific cytotoxic activity against the target cells expressing CEA. Furthermore, in the CEA transgenic mouse model, the cytotoxic T cells generated in mice immunized with miPSDCs-CEA showed CEA-specific cytotoxic activity against MC38-CEA. Genetically modified iPSDCs expressing CEA is a promising tool for clinical application on vaccine therapy against gastrointestinal cancer patients.
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