Cancer vaccine therapy using iPS-derived dendritic cells expressing TAA
Project/Area Number |
26461991
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Digestive surgery
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Research Institution | Wakayama Medical University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
中森 幹人 和歌山県立医科大学, 医学部, 准教授 (10322372)
山上 裕機 和歌山県立医科大学, 医学部, 教授 (20191190)
中村 公紀 和歌山県立医科大学, 医学部, 講師 (80364090)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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Keywords | iPS細胞 / 樹状細胞 / 腫瘍抗原 / ウイルスベクター / がんワクチン / 人工多能性幹細胞 / 腫瘍抗原遺伝子 / アデノウイルスベクター / 細胞障害性Tリンパ球 / 遺伝子組み換えマウス |
Outline of Final Research Achievements |
We investigated whether genetically modified human induced pluripotent stem cell (iPSC)-derived dendritic cells (hiPSDCs) expressing carcinoembryonic antigen (CEA) could induce CEA-specific cytotoxic T cells in a human model, and whether genetically modified mouse iPSDCs (miPSDCs) expressing CEA would show an actual antitumor effect using a CEA transgenic mouse model. We differentiated hiPSDCs from iPSCs of three healthy donors and transduced the CEA cDNA into hiPSDCs. The cytotoxic T cells induced by hiPSDCs-CEA exhibited CEA-specific cytotoxic activity against the target cells expressing CEA. Furthermore, in the CEA transgenic mouse model, the cytotoxic T cells generated in mice immunized with miPSDCs-CEA showed CEA-specific cytotoxic activity against MC38-CEA. Genetically modified iPSDCs expressing CEA is a promising tool for clinical application on vaccine therapy against gastrointestinal cancer patients.
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Report
(4 results)
Research Products
(27 results)
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[Journal Article] Circular stapling versus triangulating stapling for the cervical esophagogastric anastomosis after esophagectomy in patients with thoracic esophageal cancer: A prospective, randomized, controlled trial.2017
Author(s)
Hayata K, Nakamori M, Nakamura M, Ojima T, Iwahashi M, Katsuda M, Tsuji T, Kato T, Kitadani J, Takeuchi A, Tabata H, Yamaue H.
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Journal Title
Surgery.
Volume: in press
Issue: 1
Pages: 131-138
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Expression of BRCA1, a factor closely associated with relapse-free survival, in patients who underwent neoadjuvant chemotherapy with docetaxel, cisplatin, and fluorouracil for squamous cell carcinoma of the esophagus.2017
Author(s)
Ojima T, Nakamori M, Nakamura M, Katsuda M, Hayata K, Nakamura Y, Yamaue H.
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Journal Title
Surgery Today
Volume: 47
Pages: 65-73
Related Report
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[Journal Article] Neoadjuvant Chemotherapy with Divided-dose Docetaxel, Cisplatin and Fluorouracil for Patients with Squamous Cell Carcinoma of the Esophagus.2016
Author(s)
Ojima T, Nakamori M, Nakamura M, Katsuda M, Hayata K, Kato T, Kitadani J, Tabata H, Takeuchi A, Iwahashi M, Yamaue H.
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Journal Title
Anticancer Research
Volume: 36
Pages: 829-834
Related Report
Peer Reviewed
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[Journal Article] Successful treatment of chylothorax after esophagectomy using2016
Author(s)
Tabata H, Ojima T, Nakamori M, Nakamura M, Katsuda M, Hayata K, Matsumura S, Kato T, Kitadani J, Takeuchi A, Iwahashi M, Yamaue H.
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Journal Title
Esophagus
Volume: in press
Issue: 3
Pages: 306-310
DOI
Related Report
Peer Reviewed
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[Journal Article] Laparoscopic combined resection of synchronous gastric and colorectal cancer.2015
Author(s)
Ojima T, Nakamori M, Nakamura M, Katsuda M, Iida T, Hayata K, Takifuji K, Hotta T, Yokoyama S, Matsuda K, Iwahashi M, Yamaue H.
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Journal Title
Surgical Laparoscopy, Endoscopy and Percutaneous Techniques
Volume: 25
Issue: 1
Pages: 43-46
DOI
Related Report
Peer Reviewed
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[Journal Article] Successful treatment of esophageal fistulas with endoscopic injection of alpha-cyanoacrylate monomer.2014
Author(s)
Ojima Toshiyasu, Nakamori Mikihito, Nakamura Masaki, Katsuda Masahiro, Iida Takeshi, Hayata Keiji, Takifuji Katsunari, Iwahashi Makoto, Matsumura Shuichi, Kato Tomoya, Kitadani Junya, Yamaue Hiroki
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Journal Title
Endoscopy
Volume: 46
Issue: S 01
Pages: E62-E63
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Cancer Vaccine Therapy using iPS-derived Dendritic Cells2016
Author(s)
Toshiyasu Ojima, Hiromitsu Iwamoto, Junya Kitadani, Hirotaka Tabata, Mikihito Nakamori, Masaki Nakamura, Masahiro Katsuda, Keiji Hayata, Tomoya Kato, Akihiro Takeuchi, and Hiroki Yamaue
Organizer
第54回日本癌治療学会学術集会
Place of Presentation
横浜
Year and Date
2016-10-20
Related Report
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[Presentation] Cancer Vaccine Therapy using iPS-derived Dendritic Cells2016
Author(s)
Toshiyasu Ojima, Hiromitsu Iwamoto, Junya Kitadani, Hirotaka Tabata, Mikihito Nakamori, Masaki Nakamura, Masahiro Katsuda, Keiji Hayata, Tomoya Kato, Akihiro Takeuchi, and Hiroki Yamaue
Organizer
第25回日本癌病態治療研究会
Place of Presentation
千葉
Year and Date
2016-06-08
Related Report
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[Presentation] Expression of ERCC1, TUBB3, BRCA1, and TS as predictive markers of neoadjuvant chemotherapy for squamous cell carcinoma of the esophagus2016
Author(s)
Ojima T, Nakamori M, Nakamura M, Katsuda M, Hayata K, Matsumura S, Kato T, Yamaue H.
Organizer
ASCO-GI 2016
Place of Presentation
サンフランシスコ 米国
Year and Date
2016-01-21
Related Report
Int'l Joint Research
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