| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
We conducted metabolome analysis of paired tumor(Ts) and non-tumor esophageal tissues (NTs) using CE-TOFMS). Ts and surrounding NTs were surgically excised pair-wise from 35 esophageal cancer patients. Following tissue homogenization and metabolite extraction, a total of 110 compounds were absolutely quantified by CE-TOFMS.
There were clear metabolomic differences between Ts and NTs. Lactate and citrate levels in Ts were higher and lower, respectively, than those in NTs, which corroborated the Warburg effect in Ts. Concentrations of most amino acids except glutamine were higher in Ts than in NTs, presumably due to hyperactive glutaminolysis in Ts. Concentrations of malic acid and citric acid were lower in pT3-4 than in pT1-2, suggesting down-regulated TCA cycle activity in pT3-4. We showed significantly different metabolomic characteristics between tumor and non-tumor tissues and identified a novel set of metabolites that strongly correlated with the degree of tumor progression.
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