Screening of biomarker of gastric cancer by using semiconductor-based next-gen sequencing

• Project/Area Number: 26462002
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Chiba Cancer Center (Research Institute)
• Principal Investigator: TAKIGUCHI Nobuhiro 千葉県がんセンター(研究所), 消化器外科・臨床検査部, 部長 (00261917)
• Co-Investigator(Renkei-kenkyūsha): NAGASE Hiroki 千葉県がんセンター(研究所), がん遺伝創薬研究室, 研究所長 (90322073)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 胃がん / バイオマーカー / 遺伝子変異 / 胃癌 / 癌 / 遺伝子 / 外科

Outline of Final Research Achievements

An exome analysis of cancer related more than 400 genes was applied using Ion Torrent Proton and Ion Torrent PGM sequencers in more than 100 gastric cancer cases. Non-synonymous or non-sense germ-line variants correlated with gastric cancer cases was identified as candidate risk factors of gastric cancer in our previous population study. Many somatic mutations, germ-line variants and the copy number variants were identified by the next generation sequencing analysis. Although no relationship between these variants and clinical manifestations has been detected, non-synonymous germ-line variants was identified with a higher frequency. Further analysis is needed to study the mechanisms behind increased gastric cancer risks including possible functional polymorphisms we identified.

Research Products

• [Journal Article] 2.Randomized clinical trial comparing standard diet with perioperative oral immunonutrition in total gastrectomy for gastric cancer (2017)
  • Author(s): Ida S, Hiki N, Cho H, Sakamaki K, Ito S, Fujitani K, Takiguchi N, Kawashima Y, Nishikawa K, Sasako M, Aoyama T, Honda M, Sato T, Nunobe S, Yoshikawa T
  • Journal Title: Br J Surg Volume: 104 Issue: 4 Pages: 377-383
  • DOI: 10.1002/bjs.10417
  • Peer Reviewed
• [Journal Article] A panel of autoantibodies against multiple tumor-associated antigens for detecting gastric cancer (2017)
  • Author(s): Hoshino, I., Nagata, M., Takiguchi, N., Nabeya, Y., Ikeda, A., Yokoi, S., Kuwajima, A., Tagawa, M., Matsushita, K., Yajima, S., Shimada, H.
  • Journal Title: Cancer Sci Volume: 108 Issue: 3 Pages: 308-315
  • DOI: 10.1111/cas.13158
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 5.Feasibility of weekly intraperitoneal versus intravenous paclitaxel therapy delivered from the day of radical surgery for gastric cancer: a preliminary safety analysis of the INPACT study, a randomized controlled trial (2017)
  • Author(s): Kodera Y, Takahashi N, Yoshikawa T, Takiguchi N, Fujitani K, Ito Y, Miyamoto K, Takayama O, Imano M, Kobayashi D, Miyashita Y, Morita S, Sakamoto J
  • Journal Title: Gastric Cancer Volume: 20 Issue: 1 Pages: 190-199
  • DOI: 10.1007/s10120-016-0598-0
  • Peer Reviewed / Open Access
• [Journal Article] The Present Status of Surgical and Endoscopic Treatment for Gastric Cancer Patients Aged 85 Years or Older (2016)
  • Author(s): Takiguchi N, Nabeya Y, Shimazaki R, Ikeda A, Soda H, Tonooka T, Kainuma O, Imanishi S, Arimitsu H, Kobayashi R, Chibana T, Ishige F, Yamamoto H, Nagata M, Suzuki T.
  • Journal Title: Gan To Kagaku Ryoho. Volume: 43 Pages: 1508-1511
  • Peer Reviewed
• [Journal Article] 4.Validity of the Japanese version of functional assessment of cancer therapy-gastric (FACT-Ga) and its sensitivity to ascites volume change: a retrospective analysis of Japanese clinical trial participants. (2016)
  • Author(s): Maeda H, Sato M, Kobayashi M, Takiguchi N, Yoshikawa T, Yoshino S, Yoshida K, Tsuburaya A, Sakamoto J, Morita S
  • Journal Title: Support Care Cancer Volume: 24 Issue: 11 Pages: 4515-4521
  • DOI: 10.1007/s00520-016-3290-3
  • Peer Reviewed
• [Journal Article] 6.Specific Features of Dumping Syndrome after Various Types of Gastrectomy as Assessed by a Newly Developed Integrated Questionnaire, the PGSAS-45 (2016)
  • Author(s): Tanizawa Y, Tanabe K, Kawahira H, Fujita J, Takiguchi N, Takahashi M, Ito Y, Mitsumori N, Namikawa T, Oshio A, Nakada K
  • Journal Title: Dig Surg Volume: 33 Issue: 2 Pages: 94-103
  • DOI: 10.1159/000442217
  • Peer Reviewed
• [Journal Article] NY-ESO- autoantibody as a tumor-specific biomarker for esophageal cancer screening in 969 patients with various cancers. (2016)
  • Author(s): Y. Oshima, H. Shimada, S. Yajima, T. Nanami, K. Matsushita, F. Nomura, O. Kainuma, N Takiguchi, H. Soda, T. Ueda, T. Iizasa, . N. Yamamoto, H. Yamamoto, M. Nagata, S. Yokoi, M. M. Tagawa, S. Ohtsuka, A. Kuwajima, A. Murakami, H. Kaneko.
  • Journal Title: J Gastroenterol Volume: 51 Pages: 30-34
  • Peer Reviewed
• [Journal Article] Specific Features of Dumping Syndrome after Various Types of Gastrectomy as Assessed by a Newly Developed Integrated Questionnaire, the PGSAS-45 (2016)
  • Author(s): Y. Tanizawa, K. Tanabe, H. Kawahira, J. Fujita, N. Takiguchi, M. Takahashi, Y. Ito, N. Mitsumori, T. Namikawa, A. Oshio, K. Nakada
  • Journal Title: Dig Surg Volume: 33 Pages: 94-103
• [Journal Article] Receptor-type protein tyrosine phosphatase κ directly dephosphorylates CD133 and regulates downstream AKT activation. (2015)
  • Author(s): O. Shimozato, M. Waraya, K. Nakashima, H. Souda, N. Takiguchi, H. Yamamoto, H Takenobu, H. Uehara, E. Ikeda, S. Matsushita, N. Kubo, A. Nakagawara, T. Ozaki, T. Kamijo;
  • Journal Title: Oncogene. Volume: 34 Pages: 1949-1960
  • Peer Reviewed
• [Journal Article] 胃リンパ球浸潤癌の治療戦略 (2015)
  • Author(s): 小林亮介, 滝口伸浩, 鍋谷圭宏, 池田篤, 早田浩明, 貝沼修, 外岡亨, 今西俊介, 有光秀仁, 知花朝史, 石毛文隆, 佐々木亘亮, 山本宏
  • Journal Title: 癌と化学療法 Volume: 42 Pages: 2021-2023
  • Peer Reviewed
• [Journal Article] Randomised phase III trial of adjuvant chemotherapy with oral uracil and tegafur plus leucovorin versus intravenous fluorouracil and levofolinate in patients with stage III colorectal cancer who have undergone Japanese D2/D3 lymph node dissection: final results of JCOG0205. (2015)
  • Author(s): Shimada Y, Hamaguchi T, Mizusawa J, Saito N, Kanemitsu Y, Takiguchi N, Ohue M, Kato T, Takii Y, Sato T, Tomita N, Yamaguchi S, Akaike M, Mishima H, Kubo Y, Nakamura K, Fukuda H, Moriya Y
  • Journal Title: Eur J Cancer. Volume: 50 Issue: 13 Pages: 2231-2240
  • DOI: 10.1016/j.ejca.2014.05.025
  • Peer Reviewed
• [Journal Article] Long-term quality-of-life comparison of total gastrectomy and proximal gastrectomy by Postgastrectomy Syndrome Assessment Scale (PGSAS-45): a nationwide multi-institutional study. (2015)
  • Author(s): Takiguchi N, Takahashi M, Ikeda M, Inagawa S, Ueda S, Nobuoka T, Ota M, Iwasaki Y, Uchida N, Kodera Y, Nakada K.
  • Journal Title: Gastric Cancer. Volume: 18 Pages: 407-416
  • NAID: 40020580996
  • Peer Reviewed
• [Journal Article] Sequential paclitaxel followed by tegafur and uracil (UFT) or S-1 versus UFT or S-1 monotherapy as adjuvant chemotherapy for T4a/b gastric cancer (SAMIT): a phase 3 factorial randomised controlled trial (2014)
  • Author(s): Tsuburaya A, Yoshida K, Kobayashi M, Yoshino S, Takahashi M, Takiguchi N, Tanabe K,Takahashi N, Imamura H, Tatsumoto N, Hara H, Nishikawa K, Fukushima R, Nozaki I, Kojima H, Miyashita Y, Oba K, Buyse M, Morita M, Sakamoto J
  • Journal Title: Lancet Oncology Volume: 15 Issue: 8 Pages: 886-893
  • DOI: 10.1016/s1470-2045(14)70025-7
  • Peer Reviewed / Open Access
• [Journal Article] Double-blind, placebo-controlled, randomized phase II study of TJ-14 (hangeshashinto) for gastric cancer chemotherapy-induced oral mucositis. (2014)
  • Author(s): Aoyama T, Nishikawa K, Takiguchi N, Tanabe K, Imano M, Fukushima R, Sakamoto J, Oba MS, Morita S, Kono T, Tsuburaya A:
  • Journal Title: Cancer chemotherapy and pharmacology Volume: 73(5) Issue: 5 Pages: 1047-1054
  • DOI: 10.1007/s00280-014-2440-x
  • Peer Reviewed
• [Journal Article] Long-term monitoring of serum p53 antibody after neoadjuvant chemotherapy and surgery for esophageal adenocarcinoma: report of a case. (2013)
  • Author(s): Shimada H, Nagata M, Cho A, Takiguchi N, Kainuma O, Soda H, Ikeda A, Nabeya Y, Yajima S, Yamamoto H, Sugiyama T, Itami M.
  • Journal Title: Surgery today Volume: Epub ahead of print Issue: 10 Pages: 1957-1961
  • DOI: 10.1007/s00595-013-0787-y
  • Peer Reviewed
• [Presentation] The Present Status of the Surgical and Endoscopic Treatment for the Gastric Cancer Patients Aged 85 Years or Older (2016)
  • Author(s): Nobuhiro Takiguchi, Takuto Suzuki*, Reiri Shimazaki, Yoshihiro Nabeya, Atsushi Ikeda, Hiroaki Soda, Toru Tonooka, Hidehito Arimitsu, Tomofumi Chibana, Hiroo Yanagimashi, Matsuo Nagata
  • Organizer: 第40回国際外科学会世界大会
  • Place of Presentation: 京都
  • Year and Date: 2016-10-25
  • Int'l Joint Research
• [Presentation] 前向きコホート研究による胃がん高リスク群でのアミノ酸変化を伴う多型の解析 (2016)
  • Author(s): 滝口伸浩、中村洋子、三上春夫、永瀬浩喜
  • Organizer: 第54回日本癌治療学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2016-10-22
• [Presentation] Modified double tract再建による噴門側胃切除術 留意点と成績 (2016)
  • Author(s): 滝口伸浩、鍋谷圭宏、池田 篤、早田浩明、外岡 亨、有光秀仁、知花朝史、榛澤侑介、高山 亘、千葉 聡、星野 敢、柳橋浩男、永田松夫
  • Organizer: 第71回日本消化器外科学会総会
  • Place of Presentation: 徳島
  • Year and Date: 2016-07-14
• [Presentation] Laparoscopic proximal gastrectomy with modified double tract reconstruction; How to increase gastric inflow (2016)
  • Author(s): N. Takiguchi, M. Nagata, Y. Nabeya, A. Ikeda, O. Kainuma,
  • Organizer: The Korea International Gastric Cancer Week 2016
  • Place of Presentation: ソウル
  • Year and Date: 2016-04-21
  • Int'l Joint Research
• [Presentation] 1．進行胃癌に対する術前化学療法を併用した胃癌集学的治療の成績と課題 (2016)
  • Author(s): 滝口 伸浩, 鍋谷 圭宏, 池田 篤, 貝沼 修, 早田 浩明, 外岡 亨, 今西 俊介, 有光 秀仁, 小林 亮介, 佐々木 亘亮, 山本 宏
  • Organizer: 第116回日本外科学会定期学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2016-04-16
  • Int'l Joint Research
• [Presentation] P0Cy1胃癌に対する集学的治療 (2016)
  • Author(s): 滝口伸浩, 鍋谷圭宏, 池田 篤, 貝沼 修, 早田浩明 外岡 亨, 今西俊介, 有光秀仁, 小林亮介, 知花朝史,石毛文隆, 佐々木亘亮, 永田松夫, 山本 宏
  • Organizer: 第88回日本胃癌学会総会
  • Place of Presentation: 別府
  • Year and Date: 2016-03-17
• [Presentation] S1＋CDDP Neoadjuvant chemotherapy施行進行胃癌症例の予後曲線の検討 (2015)
  • Author(s): 滝口伸浩, 鍋谷圭宏, 池田 篤, 貝沼 修, 早田浩明, 外岡 亨, 今西俊介, 有光秀仁, 小林亮介,知花朝史, 石毛文隆, 佐々木亘亮, 永田松夫, 山本 宏
  • Organizer: 第53回日本癌治療学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2015-10-29
• [Presentation] Modified S-1 +CDDPによる胃癌Neoadjuvant chemotherapy (2015)
  • Author(s): 滝口 伸浩, 鍋谷 圭宏, 池田 篤
  • Organizer: JDDW2015
  • Place of Presentation: 東京
  • Year and Date: 2015-10-08
• [Presentation] Feasibility of weekly intraperitoneal versus intravenous paclitaxel delivered from the day of radical surgery for gastric cancer: a preliminary safety analysis of INPACT study, a randomized controlled trial. (2015)
  • Author(s): N. Takiguchi, Y. Kodera, N. Takahashi, T. Yoshikawa, K. Fujitani, S. Ito, K. Miyamoto, O.Takayama, M. Imano, D. Kobayashi, Y. Miyashita, S. Morita, J. Sakamoto
  • Organizer: European Cancer Congress 2015
  • Place of Presentation: ウィーン・オーストリア
  • Year and Date: 2015-09-25
  • Int'l Joint Research
• [Presentation] Intraperitoneal chemotherapy by CDDP for peritoneal recurrent gastric cancer patients with surgical intervention (2015)
  • Author(s): N. Takiguchi, Y. Nabeya, A. Ikeda, O. Kainuma, H. Soda, Tonooka, H Saito, H Yanagibashi, H. Arimitsu,
  • Organizer: World Congress of Surgery 2015
  • Place of Presentation: バンコク・タイ
  • Year and Date: 2015-08-23
  • Int'l Joint Research
• [Presentation] Diagnosis and treatment of Gastric GIST in our hospital (2015)
  • Author(s): N Takiguchi, M Nagata, Y Nabeya, A Ikeda, O Kainuma,
  • Organizer: 11th International Gastric Cancer Congress
  • Place of Presentation: Paulo, Brazil
  • Year and Date: 2015-06-04
  • Int'l Joint Research
• [Presentation] Clinicopathological findings of gastric mucinous adenocarcinoma (2015)
  • Author(s): Y. Tokoro, N. Takiguchi
  • Organizer: 11th International Gastric Cancer Congress
  • Place of Presentation: Paulo, Brazil
  • Year and Date: 2015-06-04
  • Int'l Joint Research
• [Presentation] Laparoscopic total gastrectomy with Roux en Y reconstruction; the benefits of intracorporeal anastomosis by hand-sewn purse-string suture (2014)
  • Author(s): N. Takiguchi, M. Nagata, Y. Nabeya, A. Ikeda, O. Kainuma, H. Soda, A. Cho, T. Tonooka, H. Arimitsu, H. Yanagibashi, H. Yamamoto
  • Organizer: 24th IASGO
  • Place of Presentation: Vienn (Austria)
  • Year and Date: 2014-12-05 – 2014-12-07
• [Presentation] 噴門側胃切除術の再建法の相違による食道逆流の発言と逆流防止の工夫 (2014)
  • Author(s): 滝口伸浩，永田松夫，鍋谷圭宏，池田 篤，貝沼 修 早田浩明，趙 明浩，外岡 亨，斉藤洋茂，有光秀仁柳橋浩男，小林亮介，知花朝史，所 為然，山本 宏
  • Organizer: 第44回胃外科・術後障害研究会
  • Place of Presentation: ロゼシアター(静岡県・富士市)
  • Year and Date: 2014-10-30 – 2014-10-31
• [Presentation] Oncologic emergency surgery occurred during chemotherapy for metastatic colorectal cancer (2014)
  • Author(s): Nobuhiro Takiguchi, Matsuo Nagata, Yoshihiro Nabeya, Atsushi Ikeda, Osamu Kainuma, Hiroaki Soda, Akihiro Cho, Toru Tonoka , Hiroshige Saito, Hidehito Arimitsu, Hiroo Yanagibashi, Ryousuke Kobayashi, Asahumi Chibana, Yukinari Tokoro, Hiroshi Yamamoto
  • Organizer: 9th Scientific and Annual Meeting of the European Society of Coloproctology ESCP2014
  • Place of Presentation: Barcelona (Spain)
  • Year and Date: 2014-09-24 – 2014-09-26
• [Presentation] 腹腔鏡下胃全摘術におけるRoux en Y再建法の手技の工夫と留意点 再建手技の変遷と成績 (2014)
  • Author(s): 滝口 伸浩, 永田 松夫, 鍋谷 圭宏, 池田 篤, 貝沼 修, 早田 浩明, 趙 明浩, 外岡 亨, 武藤 頼彦, 山本 宏
  • Organizer: 第69回日本消化器外科学会総会
  • Place of Presentation: 郡山市民文化センター、ホテルハマツ、郡山総合体育館 (福島県・郡山市)
  • Year and Date: 2014-07-16
• [Presentation] 胃癌術後腹膜播種再発に対する手術治療をともなったCDDP腹腔内化学療法 (2014)
  • Author(s): 滝口伸浩, 鍋谷圭宏, 池田 篤, 貝沼 修, 早田浩明 趙 明浩, 外岡 亨, 斉藤洋茂, 柳橋浩男, 有光秀仁 小林亮介, 知花朝史, 所 為然, 永田松夫, 山本 宏
  • Organizer: 第36回日本癌局所療法研究会
  • Place of Presentation: 八尾市文化会館プリズムホール (大阪府・八尾市)
  • Year and Date: 2014-06-27
• [Presentation] ダブルトラクト再建法による腹腔鏡下噴門側胃切除術 手技の変遷と成績 (2014)
  • Author(s): 滝口 伸浩, 永田 松夫, 鍋谷 圭宏, 池田 篤, 貝沼 修, 早田 浩明, 趙 明浩, 外岡 亨, 武藤 頼彦, 柳橋 浩男, 升田 貴仁, 山本 宏
  • Organizer: 第114回日本外科学会総会
  • Place of Presentation: グランドプリンスホテル京都 (京都府・京都市)
  • Year and Date: 2014-04-05