Analysis of CD11c+ cells in human intestinal lamina propria
| Project/Area Number |
26462014
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
香山 尚子 大阪大学, 医学系研究科, 助教 (40548814)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ヒト腸管粘膜固有細胞 / CD103+CD14-細胞 / 炎症性腸疾患 / 潰瘍性大腸炎 / CD14-CD11c+細胞 / Treg / 樹状細胞 / ヒト腸管粘膜固有層 / CD11c |
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| Outline of Final Research Achievements |
Normal intestinal mucosa was obtained from intact sites of colorectal cancer patients. Non-inflamed and inflamed colonic tissues were obtained from surgically resected specimens of UC patients. Among Lin-CD45+HLA-DRhigh intestinal lamina propria cells, CD103+ cells were sorted and analyzed. CD103+ cells in the normal colon showed lower expression of pro-inflammatory cytokines than CD103-CD14+ cells. Co-culture with naïve T cells revealed that CD103+ DCs generated Treg cells. CD103+ DCs from UC patients did not generate Treg cells, but they induced Th1/Th2/Th17 cells and showed higher expression of IL6, IL23A, IL12p35, and TNF. These findings show how human CD103+ DCs could contribute to the pathogenesis of UC.
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Report
(4 results)
Research Products
(4 results)
