| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Outline of Final Research Achievements |
Normal intestinal mucosa was obtained from intact sites of colorectal cancer patients. Non-inflamed and inflamed colonic tissues were obtained from surgically resected specimens of UC patients. Among Lin-CD45+HLA-DRhigh intestinal lamina propria cells, CD103+ cells were sorted and analyzed. CD103+ cells in the normal colon showed lower expression of pro-inflammatory cytokines than CD103-CD14+ cells. Co-culture with naïve T cells revealed that CD103+ DCs generated Treg cells. CD103+ DCs from UC patients did not generate Treg cells, but they induced Th1/Th2/Th17 cells and showed higher expression of IL6, IL23A, IL12p35, and TNF. These findings show how human CD103+ DCs could contribute to the pathogenesis of UC.
|
