Isolation and functional analysis of blood circulating tumor cells in colorectal cancer using modified Telomescan system for clinical application

• Project/Area Number: 26462025
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Juntendo University
• Principal Investigator: Ochiai Takumi 順天堂大学, 医学部, 客員准教授 (10626815)
• Co-Investigator(Kenkyū-buntansha): 小見山 博光 順天堂大学, 医学部, 非常勤講師 (30348982) ; 十合 晋作 順天堂大学, 医学部, 准教授 (80365634)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 大腸癌 / 血中循環癌細胞 / テロメラーゼ / 予後予測因子 / 治療効果判定 / 大腸がん / 腫瘍マーカー / CTC / テロメア / 遠隔転移 / ﾃﾛﾒｽｷｬﾝ法

Outline of Final Research Achievements

TelomeScan system, developed in Japan,is a method to detect circulating tumor cells (CTC) in peripheral blood by the activity of telomerase which shows high expression in cancer cells. The authors aimed to improve diagnosis of colorectal cancer by CTC detection through application of the TelomeScan system. Density of CTC in blood drawn from each colorectal cancer patients before and after neoadjuvant chemotherapy (NAC). Using this system, more CTCs were detected than with other conventional methods we tested. Density of CTC was seemed to reduced after NACs. It was suggested that CTCs in colorectal cancer should be sensitively detected with the TelomeScan system.

Research Products

• [Journal Article] Pharmacological targeting of neutrophil serine proteases prevents lethality in dextran sulfate sodium (DSS)-induced colitis in mice (2018)
  • Author(s): Ro H, Munakata S, Ueyama T, Komiyama H, Takahashi M, Kojima Y, Tomiki Y, Sakamoto K
  • Journal Title: Journal of Gastrointestinal & Digestive System Volume: 8 Issue: 01 Pages: 551-551
  • DOI: 10.4172/2161-069x.1000551
  • Peer Reviewed / Open Access
• [Journal Article] Survival benefit of lymph node dissection in surgery for colon cancer in elderly patients:multicenter propensity score-matched study in Japan. (2018)
  • Author(s): Takahashi M, Niitsu H, Sakamoto K, Hinoi T, Hattori M, Goto M, Bando H, Hazama S, Maeda K, Okita K, Horie H, Watanabe M; Japan Society of Laparoscopic Colorectal Surgery.
  • Journal Title: Asian J Endosc Surg Volume: 2018 Mar 30 Issue: 4 Pages: 1-9
  • DOI: 10.1111/ases.12474
  • Peer Reviewed
• [Journal Article] High-sensitivity detection of micrometastases generated by GFP lentivirus-transduced organoidscultured from a patient-derived colon tumor (2018)
  • Author(s): Okazawa Y, Mizukoshi K, Koyama Y, Okubo S, Komiyama H, Kojima Y, Goto M, Habu S, Hino O,Sakamoto K, Orimo A.
  • Journal Title: Journal of Visualized Experiments（JoVE） in press Volume: --
  • Peer Reviewed
• [Journal Article] Impact of the individualization of the first-line chemotherapy for advanced colorectal cancer based on collagen gel droplet-embedded drug sensitivity test. (2017)
  • Author(s): Ochiai T, Nishimura K, Watanabe T, Kitajima M, Nakatani A, Nagayasu K, Naito S, Sato T, Kishine K, Abe Y, Hara C, Yamada S, Mashiko S, Nagaoka I.
  • Journal Title: Oncol Lett. Volume: 14 Pages: 6045-6052
  • DOI: 10.3892/ol.2017.6960
  • Peer Reviewed / Open Access
• [Journal Article] Efficacy and safety of neoadjuvant chemotherapy with oxaliplatin, 5-fluorouracil, and levofolinate for T3 or T4 stage II/III rectal cancer: The FACT trial (2017)
  • Author(s): Koike J, Funahashi K, Yoshimatsu K, Yokomizo H, Kan H, Yamada T, Ishida H, Ishibashi K, Saida Y, Enomoto T, Katsumata K, Hisada M, Hasegawa H, Koda K, Ochiai T, Sakamoto K, Shiokawa H, Ogawa S, Itabashi M, Kameoka S.
  • Journal Title: Chemother Pharmacol Volume: 79 Issue: 3 Pages: 519
  • DOI: 10.1007/s00280-017-3243-7
  • Peer Reviewed / Open Access
• [Journal Article] Sensitive detection of viable circulating tumor cells using a novel conditionally telomerase-selective replicating adenovirus in non-small cell lung cancer patients. (2017)
  • Author(s): Togo S, Katagiri N, Namba Y, Tulafu M, Nagahama K, Kadoya K, Takamochi K, Oh S, Suzuki K, Sakurai F, Mizuguchi H, Urata Y, Takahashi K.
  • Journal Title: Oncotarget Volume: 4 Issue: 21 Pages: 16818-16818
  • DOI: 10.18632/oncotarget.16818
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Decrease in an anti-ageing factor, growth differentiation factor 11, in chronic obstructive pulmonary disease. (2017)
  • Author(s): Onodera K, Sugiura H, Yamada M, Koarai A, Fujino N, Yanagisawa S, Tanaka R, Numakura T, Togo S, Sato K, Kyogoku Y, Hashimoto Y, Okazaki T, Tamada T, Kobayashi S, Yanai M, Miura M, Hoshikawa Y, Okada Y, Suzuki S, Ichinose M.
  • Journal Title: Thorax Volume: 2017 Issue: 10 Pages: 893-904
  • DOI: 10.1136/thoraxjnl-2016-209352
  • Peer Reviewed / Open Access
• [Journal Article] Expression of thymidylate synthase predicts clinical outcomes of S-1-based chemotherapy in squamous cell lung cancer. (2017)
  • Author(s): Honma Y, Togo S, Shimizu K, Tulafu M, Hayashi T, Uekusa T, Tominaga S, Kido K, Fujimoto Y, Nanba Y, Takamochi K, Oh S, Suzuki K, Takahashi K.
  • Journal Title: Oncol Lett Volume: 14(3) Issue: 3 Pages: 3319-3326
  • DOI: 10.3892/ol.2017.6610
  • Peer Reviewed
• [Journal Article] CD73 as a therapeutic target for pancreatic neuroendocrine tumor stem cells. (2016)
  • Author(s): Katsuta, E., Tanaka, S., Mogushi, K., Shimada, S., Akiyama, Y., Aihara, A., Matsumura, S., Mitsunori, Y., Ban, D., Ochiai, T., Kudo, A., Fukamachi, H., Tanaka, H., Nakayama, K., Arii, S., Tanabe, M.
  • Journal Title: Int. J. Oncol. Volume: 48 Issue: 2 Pages: 657-669
  • DOI: 10.3892/ijo.2015.3299
  • Peer Reviewed / Open Access
• [Journal Article] An impact of Chromosome 17q deletion in the primary lesion of colorectal cancer on liver metastasis. (2016)
  • Author(s): Kawai M, Komiyama H, Hosoya M, Okubo H, Fujii T, Yokoyama N, Sato C, Ueyama T, Okuzawa A, Goto M, Kojima Y, Takahashi M, Sugimoto K, Ishiyama S, Munakata S, Ogura D, Niwa S, Tomiki Y, Ochiai T, Sakamoto K:
  • Journal Title: Oncology Letters Volume: 12(6) Issue: 6 Pages: 4773-4778
  • DOI: 10.3892/ol.2016.5271
  • Peer Reviewed / Open Access
• [Journal Article] Efficient detection of human circulating tumor cells without significant production of false-positive cells by a novel conditionally replicating adenovirus. (2016)
  • Author(s): Sakurai F, Narii N, Tomita K, Togo S, Takahashi K, Machitani M, et al.
  • Journal Title: Mol Ther Methods Clin Dev. Volume: 3 Pages: 16001-16001
  • DOI: 10.1038/mtm.2016.1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] StageIII結腸・直腸S状部癌における決定期分析を用いたデータマイニングによる予後解析. (2016)
  • Author(s): 杉本起一，丹羽浩一郎，石山 隼，神山博彦，小見山博光，高橋 玄，小島 豊，五藤倫敏，冨木裕一，坂本一博
  • Journal Title: 日本外科系連合学会雑誌 Volume: 40
  • Peer Reviewed / Open Access
• [Journal Article] Inhibition of Plasmin Protects Against Colitis in Mice by Suppressing Matrix Metalloproteinase 9-Mediated Cytokine Release From Myeloid Cells. (2015)
  • Author(s): Munakata S, Tashiro Y, Nishida C, Sato A, Komiyama H, Shimazu H, Dhahri D, Salama Y, Eiamboonsert S, Takeda K, Yagita H, Tsuda Y, Okada Y, Nakauchi H, Sakamoto K, Heissig B, Hattori K.
  • Journal Title: Gastroenterology Volume: 148 Issue: 3 Pages: 565-78
  • DOI: 10.1053/j.gastro.2014.12.001
  • Peer Reviewed / Open Access
• [Journal Article] Single-incision laparoscopic ｃolostomy or ileostomy for unresectable colorectal cancer (2015)
  • Author(s): Kawai M, Sakamoto K, Tomiki Y, Okuzawa A, Goto M, Kojima Y, Takahashi M, Komiyama H, Sugimoto K, Ishiyama S, Niwa K, Tashiro Y, Munakata S, Takehara K
  • Journal Title: Juntendo Medical Journal Volume: 58（6） Pages: 153-157
  • NAID: 130005093621
  • Peer Reviewed
• [Journal Article] An effective 5-fluorouracil, levofolinate, and oxaliplatin therapy for recurrent breast cancer: a case report (2014)
  • Author(s): Takahashi M, Niwa K, Ishiyama S, Sugimoto K, Komiyama H, Yaginuma Y, Kojima Y, Goto M, Okuzawa A, Tomiki Y, Sakamoto
  • Journal Title: J Med Case Rep Volume: 8 Pages: 1-6
  • Open Access
• [Journal Article] Mechanism underlying the transient increase of serum iron during FOLFOX/FOLFIRI therapy (2014)
  • Author(s): TAKUMI OCHIAI, KAZUHIKO NISHIMURA, TOMOO WATANABE, MASAYUKI KITAJIMA, AKINORI NAKATANI, TSUYOSHI SATO, KENJI KISHINE, SHUNJI FUTAGAWA, SATOMI MASHIKO, and ISAO NAGAOKA
  • Journal Title: Mol Clin Oncol Volume: 2(6) Issue: 6 Pages: 968-972
  • DOI: 10.3892/mco.2014.385
  • Peer Reviewed / Open Access
• [Presentation] 大腸癌化学療法における5FUの血中濃度測定の有用性に関する検討 (2018)
  • Author(s): 安部 雄，柵山 尚紀，藤原 憲太郎，原 千紘，佐藤 剛，根岸 健二，永易 希一，中谷 晃典，北島 政幸，渡部 智雄，落合 匠，西村 和彦，長岡 功
  • Organizer: 第118回日本外科学会定期学術集会
• [Presentation] Semaphorin 3C has a possibility of a new prognostic marker in colorectal cancer. (2017)
  • Author(s): Kawano S, Komiyama H, Sakamoto K et al.
  • Organizer: 110th AACR, Washington DC
  • Int'l Joint Research
• [Presentation] Production of monoclonal antibodies against amino acid transporter SLC7A1 to develop novel therapy for colorectal cancer. (2017)
  • Author(s): Fukaya M, Komiyama H, Sugimoto K, Kamiyama H, Sakamoto K:
  • Organizer: 第76回日本癌学会学術総会, 横浜
• [Presentation] Identification of a novel diagnostic marker for colon cancer through comprehensive microRNA analysis. (2017)
  • Author(s): Tsukamoto R, Komiyama H, Hosoya M, Kure K, Okazawa Y, Mizukoshi K, Fukaya M, Sugimoto K, Kamiyama H, Tomiki Y, Sakamoto K:
  • Organizer: 第76回日本癌学会学術総会, 横浜
• [Presentation] PAI-1 is therapeutic target molecule for prevention of postoperative adhesion formation. (2017)
  • Author(s): 本庄薫平, 雨宮浩太, 植山孝恵, 小見山博光, 石山隼, 杉本起一, 神山博彦, 高橋玄, 小島豊, 五藤倫敏, 冨木裕一, 福永哲, 梶山美明, 川崎誠治, Beate Heissig, 服部浩一, 坂本一博:
  • Organizer: 第117回日本外科学会定期学術集会, 横浜
• [Presentation] ポリマー製マイクロ流体チップを用いた大腸癌患者における CTCの測定の有用性について (2017)
  • Author(s): 土谷祐樹 呉 一眞 細谷 理樹 大久保はるな 深谷 緑 植山孝恵 河野眞吾 石山 隼 杉本起一 神山博彦 小見山博光 高橋 玄 小島 豊 奥澤淳司 冨木裕一 大永 崇 坂本一博:
  • Organizer: 第87回 大腸癌研究会
• [Presentation] 網羅的マイクロRNA解析を用いた 大腸癌新規診断マーカーの探索 (2017)
  • Author(s): 塚本亮一，萩原俊昭，雨宮浩太，土谷祐樹，松澤宏和，茂木俊介，牧野有里香，呉 一眞，本庄薫平，盧 尚志，河野眞吾，杉本起一，小見山博光，高橋 玄，小島 豊，奥澤淳司，坂本一博:
  • Organizer: 第25回日本消化器関連学会週間 福岡
• [Presentation] 患者大腸癌由来PDXおよびオルガノイドを使用した転移モデルの樹立. (2017)
  • Author(s): 水越幸輔, 岡澤 裕, 小山 侑, 大久保 捷奇, Kaidiliavi Sulidan, 伊藤恭彦, 小見山博光, 小島 豊, 五藤倫敏, 垣生園子, 樋野興夫, 折茂 彰, 坂本一博:
  • Organizer: 第28回日本消化器癌発生学会総会, 熊本
• [Presentation] 進行・再発大腸癌化学療法中における血中5-FU濃度の検討 (2016)
  • Author(s): 若林 千草 益子 里美 渡部 智雄, 北島 政幸, 中谷 昌典, 永易 希一, 岸根 健二, 佐藤 剛, 松本 啓一, 西村 和彦, 落合 匠
  • Organizer: 第54回日本癌治療学会学術集会
  • Place of Presentation: パシフィコ横浜，神奈川県横浜市
  • Year and Date: 2016-10-20
• [Presentation] 大腸癌化学療法における5-FU血中濃度・抗癌剤感受性試験と予後の関係 (2016)
  • Author(s): 落合 匠, 西村 和彦, 安部 雄, 若林 千草
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜，神奈川県横浜市
  • Year and Date: 2016-10-06
• [Presentation] The detection of colorectal CTC by the use of polymeric microfluidic devices. (2015)
  • Author(s): 呉一眞，細谷理樹，小見山博光，冨木裕一，落合 匠，大永崇，坂本一博
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-08
• [Presentation] Patient-derived colon carcinoma cells disseminate into distant organs in various different fashions. (2015)
  • Author(s): Okazawa Y, Mizukoshi K, Komiyama H, Fujii T, Goto M, Hino O, Sakamoto K, Orimo A
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-08
• [Presentation] Metastatic seeding of patient-derived colon cancer cells maintaining epithelial or mesenchymal trait. (2015)
  • Author(s): 水越 幸輔，岡澤 裕 ，門脇奈穂美，小見山博光，小島 豊，五藤 倫敏，垣生 園子，樋野 興夫，折茂 彰 ，坂本 一博
  • Organizer: 第70回日本消化器外科学会総会
  • Place of Presentation: 浜松
  • Year and Date: 2015-07-15
• [Presentation] Comparisons of long-term outcomes for locally advanced colon cancer between laparoscopic colectomy and open colectomy; propensity score analysis. (2015)
  • Author(s): Sugimoto K, Sakamoto K, Tomiki Y, Goto M, Kojima Y, Komiyama H, Takahashi M, Kamiyama H, Ishiyama S, Niwa K, Ito S, Kawai M, Takehara K, Tashiro T, Munakata S, Aoki J, Okazawa Yu O, Takahashi R, Mizukoshi K, Ro H, Ichikawa R, Kure K, Honjyo K, Tsukamoto R.
  • Organizer: 23th International Congress of the EAES
  • Place of Presentation: Bucharest, Romania
  • Year and Date: 2015-06-03
  • Int'l Joint Research
• [Presentation] Personalized first-line chemotherapy for advanced colorectal cancer using the collagen gel droplet-embedded culture drug sensitivity test. (2015)
  • Author(s): Ochiai T, Nishimura K, Watanabe T, Kitajima M., Nakatani A, Sato T, FutagawaS.
  • Organizer: ASCO Annual Meeting
  • Place of Presentation: Chichago iｌlinoi, USA
  • Year and Date: 2015-05-29
  • Int'l Joint Research
• [Presentation] CHFR methylation is an independent prognostic marker of poor prognosis in gastric cancer. (2015)
  • Author(s): Sugimoto K, Humbert A, Lu Z, Chen C, Rodgers K, Lee,B Griffin C, Sato K, Orita H, Ito T, Komiyama H, Kojima Y, Goto M, Tomiki Y, Sakamoto K, Brock M
  • Organizer: 2015 American Association for Cancer Research Annual Meeting
  • Place of Presentation: Pennsylvania, USA
  • Year and Date: 2015-04-18
  • Int'l Joint Research
• [Presentation] Comparative Genomic Hybridization (CGH) を用いた 大腸がんの肝転移と予後因子の検 (2014)
  • Author(s): 河合雅也，小見山博光，呉 一眞，本庄薫平，盧 尚志,岡澤 裕，高橋里奈，水越幸輔，，嵩原一裕，宗像 慎也，丹羽浩一郎，石山 隼，杉本起一，神山博彦，高橋 玄，，柳沼行宏，小島 豊，五藤倫敏，，冨木裕一，坂本一博
  • Organizer: 2014 JDDW 第56回日本消化器病学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2014-10-23 – 2014-10-26
• [Presentation] 大腸癌における血清p53抗体の検討 (2014)
  • Author(s): 高橋里奈，呉 一眞，本庄薫平，盧 尚志，青木 順，岡澤 裕，，水越幸輔，河合雅也，嵩原一裕，宗像慎也，石山 隼，杉本起一，小見山博光，高橋 玄，柳沼行宏，小島 豊，五藤倫敏，奥澤淳司，，冨木裕一，坂本一博
  • Organizer: 2014 JDDW 第12回日本消化器外科学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2014-10-23 – 2014-10-26
• [Remarks] 順天堂大学大学院 下部消化管外科（消化器外科学講座）
  • URL: https://www.juntendo.ac.jp/graduate/laboratory/labo/kabusyoukakan/
• [Remarks] 順天堂大学大学院 下部消化管外科学
  • URL: https://www.juntendo.ac.jp/graduate/laboratory/labo/kabusyoukakan/
• [Remarks] 順天堂大学 下部消化管外科
  • URL: http://www.juntendo.ac.jp/graduate/laboratory/labo/kabusyoukakan/