| Project/Area Number |
26462039
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Kaneko Junichi 東京大学, 医学部附属病院, 講師 (50328118)
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| Co-Investigator(Renkei-kenkyūsha) |
INAGAKI Yoshinori 東京大学, 医学部附属病院, 助教 (40733390)
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| Research Collaborator |
URANO Yasuteru
KOKUDO Takashi
SHIRATA Chikara
KIRITANI Sho
SATO Masamitsu
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 肝細胞癌 / indocyanine green / 光線力学的治療 / 光線力学療法 / Indocyanine green / 近赤外光 / レーザー |
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| Outline of Final Research Achievements |
We developed, a new anti-cancer treatment, the photodynamic therapy (PDT) using Indocyanine green (ICG) and near-infrared (NIR) laser for hepatocellular carcinoma (HCC). However, a molecular mechanism underlying this effect needs to be elucidated. HuH-7 cells, a well-differentiated human HCC cell line, were transplanted subcutaneously into BALB/c-nu/nu mice. ICG was administered 24h before NIR irradiation. The irradiation was performed by 823-nm NIR laser. The temperature of HuH-7 xenografts rose to 49C. The cell toxicity of ICG-NIR was inhibited by cooling sheet. Reactive oxygen species (ROS) production was detected in vitro and in vivo, and the ROS production was inhibited under with a cooling sheet. Repeat NIR irradiation increased the cell toxicity. The mean tumor volume growth on day 9 was significantly suppressed (p<0.01). Apoptosis of HCC cells after ICG-NIR PDT is caused by heat production mediated ROS production. Repeated ICG-NIR irradiation improves the anti-tumor effect.
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