| Project/Area Number |
26462044
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
副島 雄二 九州大学, 医学研究院, 准教授 (30325526)
調 憲 群馬大学, 大学院医学系研究科, 教授 (70264025)
内山 秀昭 九州大学, 医学研究院, 共同研究員 (70380425)
吉住 朋晴 九州大学, 医学研究院, 准教授 (80363373)
池上 徹 九州大学, 大学病院, 助教 (80432938)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 脂肪肝 / 虚血再灌流障害 / 温虚血再灌流障害 / 温阻血再灌流傷害 |
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| Outline of Final Research Achievements |
Fatty liver rats underwent surgically induced partial hepatic ischemia followed by reperfusion. The overall survival rate after I/R was lower in rats with fatty livers than with normal livers. Necrotic area and the concentrations of 8-hydroxy-2′-deoxyguanosine (8-OHdG), TNFα, and IL-6 were higher in fatty liver tissue than in normal liver tissue. The number of p47phox-positive cells was significantly higher in fatty liver tissue than in normal liver tissue after reperfusion and peaked 24 hours after reperfusion. The number of TLR-4 positive cells was significantly higher in fatty liver tissue than in normal liver tissue after reperfusion and peaked 4 and 24 hours after reperfusion coupled with a decreased number of high-mobility group box 1-positive hepatocytes. Apocynin significantly improved the survival rate, necrotic area, and concentrations of 8-hydroxy-2′-deoxyguanosine, TNFα, and IL-6. The protective effect of apocynin on fatty livers was greater than on normal livers.
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