| Project/Area Number |
26462047
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kumamoto University |
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Principal Investigator |
TAKAMORI Hiroshi 熊本大学, 医学部附属病院, 非常勤診療医師 (90363514)
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| Co-Investigator(Kenkyū-buntansha) |
岡部 弘尚 熊本大学, 医学部附属病院, 非常勤診療医師 (40573621)
別府 透 熊本大学, 医学部附属病院, 非常勤診療医師 (70301372)
林 洋光 熊本大学, 医学部附属病院, 非常勤診療医師 (80625773)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | FXR agonist / 肝再生 / 肝切除 / FXR / 胆汁酸 / Farnesoid受容体X |
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| Outline of Final Research Achievements |
Mouse model of 70 % hepatectomy was established. Protocol was prepared for the experiment with FXR agonist. In clinical experiment with paraffin embedded section, immunohistochemistry was performed with FXR antibody (PPMX corp., Clone No. A9033A). Staining pattern was appropriate and nuclear localization of FXR was confirmed. On the other hand, nuclear localization of FXR in more than 50% of hepatocytes in background liver was seen in almost all samples (n > 50). Therefore, it was hard to assign them into two groups with/without FXR activation. Other methodology is required for evaluating activation status of FXR in hepatocytes.
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