Assessment of proliferation and apoptotic rate of tumor budding cells in pancreatic ductal adenocarcinoma.

• Project/Area Number: 26462056
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Niigata University
• Principal Investigator: Takizawa Kazuyasu 新潟大学, 医歯学総合病院, 助教 (30706437)
• Co-Investigator(Kenkyū-buntansha): 若井 俊文 新潟大学, 医歯学系, 教授 (50372470) ; 皆川 昌広 新潟大学, 医歯学総合研究科, 客員研究員 (10464009) ; 高野 可赴 新潟大学, 医歯学総合研究科, 客員研究員 (30606306) ; 小林 隆 新潟大学, 医歯学総合病院, 講師 (40464010) ; 亀山 仁史 新潟大学, 医歯学系, 准教授 (40626420) ; 小杉 伸一 新潟大学, 医歯学総合病院, 特任教授 (90401736)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 簇出 / 増殖能 / アポトーシス / 膵癌 / 細胞増殖活性 / Ki-67 / TUNEL / AE1/AE3染色

Outline of Final Research Achievements

Tumor budding is a prognostic factor in PDAC. Budding cancer cells in AE1/AE3 immunostaining were counted significantly more than in HE staining (P < 0.001). High grade budding evaluated with both standard HE and AE1/AE3 staining correlated significantly with poor overall survival and the histological grade. Multivariate analysis revealed that the margin status, lymph node metastasis, the high grade budding with proliferative potency assessed by AE1/AE3 and Ki-67 double immunostaining were independent prognostic factors. The cumulative 2-year survival rates were 63.6% for patients with negative immunostaining of tumor budding for Ki-67, 46.9% for patients with positive of low grade budding and 0% for patients with positive of high grade budding (P < 0.001).The average percentage of cases showing Ki-67 positivity was 73% and the average percentage of cases showing TUNEL positivity was only 7%, suggesting decreased apoptosis at the budding cancer cells at invasive front of the tumor.

Research Products

• [Presentation] 膵癌腫瘍先進部における簇出およびその細胞増殖活性は独立した予後不良因子である (2017)
  • Author(s): 滝沢一泰
  • Organizer: 第72回日本消化器外科学会総会
  • Place of Presentation: 石川県立音楽堂ほか(石川県金沢市)
• [Presentation] Association of tumor budding with clinicopathological characteristics and prognosis in pancreatic ductal adenocarcinoma: Impact of Ki-67 expression on budding cancer cells. (2015)
  • Author(s): Takizawa K
  • Organizer: International Association of Surgions, Gastroenterologists and Oncologists Continuing Medical Education: Advanced Post-Graduate Course in Tokyo 2015
  • Place of Presentation: The Jikei University School of Medicine (Minato-ku, Tokyo)
  • Year and Date: 2015-06-13
  • Int'l Joint Research
• [Presentation] Association of tumor budding with clinicopathological characteristics and prognosis in pancreatic ductal adenocarcinoma (2015)
  • Author(s): Takizawa K
  • Organizer: The 5th Biennial Congress of the Asian-Pacific Hepato-Pancreato-Biliary Association
  • Place of Presentation: Singapore, Suntec Singapore Convention & Exhibition Centre
  • Year and Date: 2015-03-18 – 2015-03-21