Proteomic analysis of stromal tissue of pancreas
Project/Area Number |
26462070
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Digestive surgery
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Research Institution | Yokohama City University |
Principal Investigator |
ENDO Itaru 横浜市立大学, 医学研究科, 教授 (60211091)
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Co-Investigator(Kenkyū-buntansha) |
廣島 幸彦 横浜市立大学, 医学研究科, 客員研究員 (60718021)
森 隆太郎 横浜市立大学, 医学部, 講師 (90596412)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
Keywords | 膵癌 / 浸潤 / 転移 / 免疫 / 膵炎 / Kras / CRMP4 / マウスモデル / リン酸化 / CDK5 / GSK3β |
Outline of Final Research Achievements |
Pancreatic cancer has been an aggressive malignancy. Recently, stroma in tumor is reported to be associated with cancer-cell proliferation, apoptosis, differentiation, and invasion in several cancers. Previously, we examined proteins expressing in the stroma of pancreatic tumors or of normal pancreas, and found that CRMP4 differentially expressed in pancreatic tumor stroma compared to normal pancreatic tissue. Of all the CRMPs, only CRMP4 was differentially expressed in pancreatic cancer tissues. CRMP4 staining was highly correlated with poor differentiation and liver metastasis. In this project, we revealed that caerulein-induced pancreatitis augments the expression of CRMP4 in vivo experiment. Sequencially, we investigated the role of CRMP4 in pancreatic cancer precursor lesions in gene engineered mouse model. Our results suggested that CRMP4 is significantly associated with initiation and progression of pancreatic cancer.
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Patient-derived mouse models of cancer need to be orthotopic in order to evaluate targeted anti-metastatic therapy.2016
Author(s)
Hiroshima Y, Maawy A, Zhang Y, Zhang N, Murakami T, Chishima T, Tanaka K, Ichikawa Y, Bouvet M, Endo I, Hoffman RM.
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Journal Title
Oncotarget.
Volume: 7
Issue: 44
Pages: 71696-71702
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Effective molecular targeting of CDK4/6 and IGF-1R in a rare FUS-ERG fusion CDKN2A-deletion doxorubicin-resistant Ewing's sarcoma patient-derived orthotopic xenograft (PDOX) nude-mouse model.2016
Author(s)
Murakami T, Singh AS, Kiyuna T, Dry SM, Li Y, James AW, Igarashi K, Kawaguchi K, DeLong JC, Zhang Y, Hiroshima Y, Russell T, Eckardt MA, Yanagawa J, Federman N, Matsuyama R, Chishima T, Tanaka K, Bouvet M, Endo I, Eilber FC, Hoffman RM.
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Journal Title
Oncotarget.
Volume: 7
Issue: 30
Pages: 47556-47564
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Tumor-Targeting Salmonella typhimurium A1-R Arrests a Chemo-Resistant Patient Soft-Tissue Sarcoma in Nude Mice.2015
Author(s)
Hiroshima Y, Zhao M, Zhang Y, Zhang N, Maawy A, Murakami T, Mii S, Uehara F, Yamamoto M, Miwa S, Yano S, Momiyama M, Mori R, Matsuyama R, Chishima T, Tanaka K, Ichikawa Y, Bouvet M, Endo I, Hoffman RM.
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Journal Title
PLoS One
Volume: 10
Issue: 8
Pages: e0134324-e0134324
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Metastatic Recurrence in a Pancreatic Cancer Patient Derived Orthotopic Xenograft (PDOX) Nude Mouse Model Is Inhibited by Neoadjuvant Chemotherapy in Combination with Fluorescence-Guided Surgery with an Anti-CA 19-9-Conjugated Fluorophore.2014
Author(s)
Hiroshima Y, Maawy A, Zhang Y, Murakami T, Momiyama M, Mori R, Matsuyama R, Katz MH, Fleming JB, Chishima T, Tanaka K, Ichikawa Y, Endo I, Hoffman RM, Bouvet M.
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Journal Title
PLoS One.
Volume: 2;9(12)
Issue: 12
Pages: e114310-e114310
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Efficacy of tumor-targeting Salmonella typhimurium A1-R in combination with anti-angiogenesis therapy on a pancreatic cancer patient-derived orthotopic xenograft (PDOX) and cell line mouse models.2014
Author(s)
Hiroshima Y, Zhang Y, Murakami T, Maawy A, Miwa S, Yamamoto M, Yano S, Sato S, Momiyama M, Mori R, Matsuyama R, Chishima T, Tanaka K, Ichikawa Y, Bouvet M, Endo I, Zhao M, Hoffman RM.
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Journal Title
Oncotarget.
Volume: 15;5(23).
Pages: 12346-57
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Efficacy of Salmonella typhimurium A1-R Versus Chemotherapy on a Pancreatic Cancer Patient-Derived Orthotopic Xenograft (PDOX).2014
Author(s)
Hiroshima Y, Zhao M, Maawy A, Zhang Y, Katz MH, Fleming JB, Uehara F, Miwa S, Yano S, Momiyama M, Suetsugu A, Chishima T, Tanaka K, Bouvet M, Endo I, Hoffman RM.
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Journal Title
J Cell Biochem
Volume: 115(7)
Issue: 7
Pages: 1254-61
DOI
Related Report
Peer Reviewed
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[Journal Article] Successful fluorescence-guided surgery on human colon cancer patient-derived orthotopic xenograft mouse models using a fluorophore-conjugated anti-CEA antibody and a portable imaging system.2014
Author(s)
Hiroshima Y, Maawy A, Metildi CA, Zhang Y, Uehara F, Miwa S, Yano S, Sato S, Murakami T, Momiyama M, Chishima T, Tanaka K, Bouvet M, Endo I, Hoffman RM.
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Journal Title
J Laparoendosc Adv Surg Tech A.
Volume: 24(4)
Issue: 4
Pages: 241-7
DOI
Related Report
Peer Reviewed
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[Journal Article] The tumor-educated-macrophage increase of malignancy of human pancreatic cancer is prevented by zoledronic acid.2014
Author(s)
Hiroshima Y, Maawy A, Hassanein MK, Menen R, Momiyama M, Murakami T, Miwa S, Yamamoto M, Uehara F, Yano S, Mori R, Matsuyama R, Chishima T, Tanaka K, Ichikawa Y, Bouvet M, Endo I, Hoffman RM.
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Journal Title
PLoS One.
Volume: 12;9(8)
Issue: 8
Pages: e103382-e103382
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Fluorescence-guided surgery of liver metastasis in orthotopic nude-mouse models2015
Author(s)
Takashi Murakami, Yukihiko Hiroshima, Shinji Miwa, Shuya Yano, Makoto Toneri, Mako Yamamoto, Masashi Momiyama, Takashi Chishima, Kuniya Tanaka, Michael Bouvet, Itaru Endo, Robert M. Hoffman
Organizer
AACR
Place of Presentation
Philadelphia, USA
Year and Date
2015-04-20
Related Report
Int'l Joint Research
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[Presentation] Curative fluorescence-guided surgery of pancreatic cancer in combination with UVC irradiation in orthotopic mouse models2015
Author(s)
Yukihiko Hiroshima, Ali Maawy, Yong Zhang, Sho Sato, Takashi Murakami, Mako Yamamoto, Fuminari Uehara, Shinji Miwa, Shuya Yano, Masashi Momiyama, Takashi Chishima, Kuniya Tanaka, Michael Bouvet, Itaru Endo, Robert M. Hoffman
Organizer
AACR
Place of Presentation
Philadelphia, USA
Year and Date
2015-04-20
Related Report
Int'l Joint Research
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[Presentation] Fluorescence-guided surgery with an anti-CA 19-9-conjugated fluorophore in combination with neoadjuvant chemotherapy inhibits metastatic recurrence in a pancreatic cancer patient derived orthotopic xenograft (PDOX) nude mouse model2015
Author(s)
Yukihiko Hiroshima1, Ali Maawy2, Yong Zhang3, Takashi Murakami4, Masashi Momiyama4, Ryutaro Mori4, Ryusei Matsuyama4, Matthew H. Katz5, Jason B. Fleming5, Takashi Chishima4, Kuniya Tanaka4, Yasushi ichikawa4, Itaru Endo4, Robert M. Hoffman6, Michael Bouvet2.
Organizer
米国癌学会(AACR annual meeting 2015)
Place of Presentation
Philadelphia、米国
Year and Date
2015-04-18 – 2015-04-22
Related Report
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