| Project/Area Number |
26462081
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cardiovascular surgery
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
酒井 俊 筑波大学, 医学医療系, 講師 (30282362)
坂本 裕昭 筑波大学, 医学医療系, 准教授 (30611115)
兵藤 一行 大学共同利用機関法人高エネルギー加速器研究機構, 物質構造科学研究所, 准教授 (60201729)
宮内 卓 筑波大学, 医学医療系, 教授 (60222329)
松下 昌之助 筑波技術大学, 保健科学部, 教授 (70359579)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2016: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 肺高血圧症 / 放射光微小血管撮影法 / シアストレス / 好感度放射光微小血管造影 / シアーストレス / 肺血管拡張薬 / 高感度放射光微小血管造影 |
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| Outline of Final Research Achievements |
We have previously established a synchrotron radiation pulmonary micro-angiography (SRPA) in an in-vivo rat model. In this study, we aimed to qualify micro-vascular remodeling in crotaline induced pulmonary arterial hypertension (PAH) rats. To determine the association of endothelial function to the vascular remodeling, local expression of endotheline-1(ET-1), endothelial nitric oxide synthase(eNOS) was evaluated by immunohistochemical staining. Microvascular density measured by SRPA was significantly decreased in the PAH rats. eNOS expression was decreased and ET-1 expression was increased significantly in the PAH rats. SRPA is a useful method to visualize decreased and narrowed pulmonary micro-vasculature in the PAH rats. Increased ET-1 expression may contribute to a proliferation and remodeling of pulmonary arterioles. Also, decreased eNOS expression may associate with vasospasm of pulmonary arterioles induced by endothelial dysfunction due to increased shear stress in PAH.
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