| Project/Area Number |
26462089
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cardiovascular surgery
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
今西 悠基子 大阪大学, 医学系研究科, 特任研究員 (10707582)
福嶌 五月 国立研究開発法人国立循環器病研究センター, 病院, 医長 (80596867)
秦 広樹 大阪大学, 医学系研究科, 助教 (80638198)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 細胞シート / ガラス化凍結 / 骨格筋芽細胞 / iPS細胞由来心筋細胞 / 再生医療 / 凍結 / 細胞保存 |
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| Outline of Final Research Achievements |
We explored the clinically applicable cryopreservation method of skeletal myoblast sheet and iPS cells-derived cardiomyocytes (CMs) sheet. As a result, the vitrified frozen human skeletal myoblast sheet maintained the functionality and structure of cell-sheet. In addition, transplantation of the thawed human myoblast sheet from the vitrified cryopreservation induced the improvement of cardiac function in the myocardial infarction (MI) nude rat.
In the iPS cell-derived CMs, the purity of CMs did not change by the vitrified frozen and the thawing process. The thawed CMs sheet showed spontaneous synchronized beatings. To check the impacts on cardiac performance in vivo, we transplanted the cell sheets of non-cryopreserved CMs and cryopreserved CMs into nude rats with MI. There was no significant difference in the efficiency between the non-cryopreserved and cryopreserved groups, indicating that the efficiency of cryopreserved CMs in vivo was comparable to that of freshly prepared CMs.
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