Analysis of phlebolymphedema in edema

• Project/Area Number: 26462103
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular surgery
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: Yamamoto Naoto 浜松医科大学, 医学部附属病院, 助教 (80402262)
• Co-Investigator(Kenkyū-buntansha): 海野 直樹 浜松医科大学, 医学部, 准教授 (20291958)
• Research Collaborator: TANAKA Hiroki ; SANO Masaki ; SAITO Takaaki ; SUGISAWA Ryota ; KATAHASHI Kazuto ; YATA Tatsuro ; KAYAMA Takafumi
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 浮腫 / リンパ浮腫 / 慢性静脈不全 / 深部静脈血栓症 / 静脈血栓症

Outline of Final Research Achievements

We investigate the effects of insuffi¬cient lymph drainage on vein walls. In rats, the lymphatic collecting vessels sur¬rounding the femoral vein were ligated, which caused the local retention of lymphatic fluid. A histological study of the fem¬oral vein and the surrounding tissue was con¬ducted. IMS was used to analyze the distribution of lipid molecules in the perivascular tissue.
After the procedure, the lymphatic vessels in the model tissue were significantly dilated. Compared with the control tissue, the model tissue exhibited marked perivascular accumulation of lysophosphatidylcholine, phosphatidylcholine, and triglycerides. The walls of the femoral veins were 3.4-fold thicker than the control. The number of TNF-α positive adipocytes was increased in the perivascular regions of the model tissue. The accumulation of lymphatic fluid changes the structure of vein walls, and such changes might be associated with chronic venous insufficiency.