Protection and regeneration of spinal cord by trehalose and mesenchymal stem cells cultured under microgravity
| Project/Area Number |
26462107
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Cardiovascular surgery
|
|---|
| Research Institution | Hiroshima University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
弓削 類 広島大学, 医歯薬保健学研究院(保), 教授 (20263676)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
|
|---|
| Keywords | 脊髄虚血 / 大動脈手術 / 脊髄保護 / 間葉系幹細胞 / 微重力培養 / ラットモデル / 宇宙療法 / 国際研究者交流 NASA |
|---|
| Outline of Final Research Achievements |
Rat spinal cord ischemia model was used in this study. 1) MSC cultured under standard gravity of 10^8 was used. Lower limb function at 6 hours and 24 hours after surgery was significantly better in the group with MSC injection compared with the group without MSC (group PBS) and sham group. RT-PCR analysis showed the ratio of Bax to Bcl-2 was significantly lower in the group MSC than the group PBS. Injected MSC was observed in the spinal cord by histological examination at 24 hours after surgery. 2)MSC cultured under microgravity of 10^6 cells had a potential to spinal cord protection. 3) The efficacy of trehahose is not still clear.
|
Report
(4 results)
Research Products
(3 results)
