Protection and regeneration of spinal cord by trehalose and mesenchymal stem cells cultured under microgravity

• Project/Area Number: 26462107
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular surgery
• Research Institution: Hiroshima University
• Principal Investigator: Takahashi Shinya 広島大学, 病院(医), 病院助教 (70423382)
• Co-Investigator(Kenkyū-buntansha): 弓削 類 広島大学, 医歯薬保健学研究院(保), 教授 (20263676)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
• Keywords: 脊髄虚血 / 大動脈手術 / 脊髄保護 / 間葉系幹細胞 / 微重力培養 / ラットモデル / 宇宙療法 / 国際研究者交流 NASA

Outline of Final Research Achievements

Rat spinal cord ischemia model was used in this study. 1) MSC cultured under standard gravity of 10^8 was used. Lower limb function at 6 hours and 24 hours after surgery was significantly better in the group with MSC injection compared with the group without MSC (group PBS) and sham group. RT-PCR analysis showed the ratio of Bax to Bcl-2 was significantly lower in the group MSC than the group PBS. Injected MSC was observed in the spinal cord by histological examination at 24 hours after surgery. 2)MSC cultured under microgravity of 10^6 cells had a potential to spinal cord protection. 3) The efficacy of trehahose is not still clear.

Research Products

• [Journal Article] Prevention of Spinal Cord Ischemia During Thoracic Endovascular Aortic Repair. (2017)
  • Author(s): Takahashi S, Sueda T.
  • Journal Title: Kyobu Geka Volume: 70 Pages: 251-256
  • Peer Reviewed
• [Journal Article] Invited commentary (2017)
  • Author(s): Takahashi S
  • Journal Title: Ann Thorac Surg Volume: 103 Issue: 3 Pages: 811-811
  • DOI: 10.1016/j.athoracsur.2016.07.034
  • Peer Reviewed
• [Presentation] 脊髄虚血再灌流モデルラットに対する間葉系幹細胞移植効果の検討 (2017)
  • Author(s): 中川 慧, 高橋信也, 猪村剛史, 大塚貴志, 富安真弓, Looniva Shrestha, 末田泰二郎, 河原裕美, 弓削 類
  • Organizer: 第16回日本再生医療学会総会
  • Place of Presentation: 仙台
  • Year and Date: 2017-03-09