Identification of biomarker for colorectal cancer metastasis to lung with CAGE analyses

• Project/Area Number: 26462136
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Respiratory surgery
• Research Institution: Juntendo University
• Principal Investigator: Yaginuma Yukihiro 順天堂大学, 医学部, 非常勤講師 (60338415)
• Co-Investigator(Kenkyū-buntansha): 小見山 博光 順天堂大学, 医学部, 非常勤講師 (30348982) ; 高持 一矢 順天堂大学, 医学部, 准教授 (30397369) ; 河合 純 国立研究開発法人理化学研究所, 予防医療・診断技術開発プログラム, 副プログラムディレクター (30391923) ; 藤井 智明 順天堂大学, 医学(系)研究科(研究院), 非常勤助教 (10511420)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: CAGE法 / 大腸癌 / 腫瘍マーカー / 肺転移 / 網羅的発現解析 / 次世代シークエンサー / 転移予測マーカー / CAGE解析 / RNA発現解析 / 大腸がん / 転移マーカー / FANTOM

Outline of Final Research Achievements

We conducted an exhaustive gene expression analysis in carcinoma tissue of colorectal cancer patients accompanied by metastases, using the cap analysis of gene expression (CAGE). There were differences in genes of which expression were promoted or suppressed between the cases with and without lung metastasis. Some gene expressions were enhanced only in cases with lung metastasis，specifically. The clinical application of genes identified in this work as a novel biomarker will enable us to detect earlier and to predict lung metastasis in colorectal cancer. It should help to promote clinical diagnosis and medical care of the cancer.

Research Products

• [Journal Article] An impact of Chromosome 17q deletion in the primary lesion of colorectal cancer on liver metastasis. (2016)
  • Author(s): Kawai M, Komiyama H, Hosoya M, Okubo H, Fujii T, Yokoyama N, Sato C, Ueyama T, Okuzawa A, Goto M, Kojima Y, Takahashi M, Sugimoto K, Ishiyama S, Munakata S, Ogura D, Niwa S, Tomiki Y, Ochiai T, Sakamoto K:
  • Journal Title: Oncology Letters Volume: 12(6) Issue: 6 Pages: 4773-4778
  • DOI: 10.3892/ol.2016.5271
  • Peer Reviewed / Open Access
• [Journal Article] Postoperative Adjuvant Chemotherapy Improves Survival in Stage II Colon Cancer ? A Propensity Score Matching Analysis (2016)
  • Author(s): Sugimoto K, Sakamoto K, Niwa K, Ishiyama S Kamiyama H, Komiyama H, Takahashi M,Kojima Y, Goto M, Tomiki Y:
  • Journal Title: Juntendo Medical Journal Volume: 62 Issue: 6 Pages: 463-471
  • DOI: 10.14789/jmj.62.463
  • NAID: 130005402125
  • ISSN: 2187-9737, 2188-2126
  • Peer Reviewed / Open Access
• [Journal Article] Novel biomarkers that assist in accurate discrimination of squamous cell carcinoma from adenocarcinoma of the lung (2016)
  • Author(s): Takamochi K, Ohmiya H, Itoh M, Mogushi K, SaitoT, Hara K, Mitani K, Kogo Y, Yamanaka Y, Kawai J, Hayashizaki Y, Oh S, Suzuki K, Kawaji H
  • Journal Title: BMC cancer Volume: 16:760 Pages: 1-10
  • Peer Reviewed / Open Access
• [Journal Article] A novel mouse model of intrahepatic cholangiocarcinoma induced by liver-specific Kras activation and Pten deletion. (2016)
  • Author(s): Ikenoue T, Terakado Y, Nakagawa H, Hikiba Y, Fujii T, Matsubara D, Noguchi R, Zhu C, Yamamoto K, Kudo Y, Asaoka Y, Yamaguchi K, Ijichi H, Tateishi K, Fukushima N, Maeda S, Koike K, Furukawa Y
  • Journal Title: Sci Rep Volume: 印刷中 Issue: 1 Pages: 23899-23899
  • DOI: 10.1038/srep23899
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] StageIII結腸・直腸S状部癌における決定期分析を用いたデータマイニングによる予後解析 (2016)
  • Author(s): 杉本起一，丹羽浩一郎，石山 隼，神山博彦，小見山博光，高橋 玄，小島 豊，五藤倫敏，冨木裕一，坂本一博
  • Journal Title: 日本外科系連合学会雑誌 (掲載決定) Volume: 41 Pages: 0000-0000
  • Peer Reviewed / Open Access
• [Journal Article] Impact of chromosome 17q deletion in the primary lesion of colorectal cancer on liver metastasis (2016)
  • Author(s): Kawai M, Komiyama H, Hosoya M, Okubo H, Tomoaki Fujii, Yokoyama, N, Sato C, Ueyama T, Okuzawa A, Goto M, Kojima Y, Takahashi, M Sugimoto K, Ishiyama S, Munakata S, Ogura,D, Niwa S, Tomiki Y Ochiai T, Sakamoto K
  • Journal Title: Oncology Letters Volume: 11 Pages: 0000-0000
• [Journal Article] Single-Incision Laparoscopic Colostomy or Ileostomy for Unresectable Colorectal Cancer (2015)
  • Author(s): Kawai M, Sakamoto K, Tomiki Y, Okuzawa A, Goto M, Kojima Y, Takahashi M, Komiyama H, Sugimoto K, Ishiyama S, Niwa K, Tashiro Y, Munakata S, Takehara K
  • Journal Title: Juntendo Medical Journal Volume: 58（6）: Pages: 153-157
  • NAID: 130005093621
  • Open Access
• [Journal Article] Inhibition of Plasmin Protects Against Colitis in Mice by Suppressing Matrix Metalloproteinase 9-Mediated Cytokine Release From Myeloid Cells. (2015)
  • Author(s): Munakata S, Tashiro Y, Nishida C, Sato A, Komiyama H, Shimazu H, Dhahri D, Salama Y, Eiamboonsert S, Takeda K, Yagita H, Tsuda Y, Okada Y, Nakauchi H, Sakamoto K, Heissig B, Hattori K.
  • Journal Title: Gastroenterology Volume: 148 Issue: 3 Pages: 565-78
  • DOI: 10.1053/j.gastro.2014.12.001
  • Peer Reviewed / Open Access
• [Journal Article] Laparoscopic right hemicolectomy after coronary artery bypass grafting using the right gastroepiploic artery: A case report (2014)
  • Author(s): Takahashi M, Aoki J, Okazawa Y, Yaginuma Y, Kojima Y, Sakamoto K
  • Journal Title: International Journal of Case Reports and Images Volume: 5 Pages: 567-571
  • Open Access
• [Journal Article] An effective 5-fluorouracil, levofolinate, and oxaliplatin therapy for recurrent breast cancer: a case report (2014)
  • Author(s): Takahashi M, Niwa K, Ishiyama S, Sugimoto K, Komiyama H, Yaginuma Y, Kojima Y, Goto M, Okuzawa A, Tomiki Y, Sakamoto
  • Journal Title: J Med Case Rep Volume: 8 Pages: 1-6
  • Open Access
• [Presentation] SLC7A1: a cationic amino acid transporter as a potential target for colorectal cancer therapy (2016)
  • Author(s): Fukaya M, Komiyama H, Tsukamoto R, Mizukoshi K, Okazawa Ym Sakamoto K:
  • Organizer: 第75回日本癌学会学術総会 2016年10月６－８
  • Place of Presentation: パシフィコ横浜（横浜市西区）
  • Year and Date: 2016-10-06
• [Presentation] Multicellular tumor clusters seed metastases in colon patientderived tumor xenografts (2016)
  • Author(s): Okubo S, Mizukoshi K, Okazawa Y, Haeno H, Komiyama H, Saeki H, Ito Y, Gotou M， Watanabe S,Hino O, Sakamoto K, Orimo A
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（横浜市西区）
  • Year and Date: 2016-10-06
• [Presentation] Polymeric CTC-chipを用いた大腸癌CTCの検出 (2016)
  • Author(s): 呉 一眞，細谷理樹，塚本亮一，伊藤慎吾, 本庄薫平，岡澤 裕, 水越幸輔, 宗像慎也, 石山 隼, 杉本起一，神山博彦，小見山博光，高橋 玄，柳沼行宏, 小島 豊, 五藤倫敏, 奥澤淳司，冨木裕一, 坂本一博， 大永 崇
  • Organizer: 第116回日本外科学会定期学術集会
  • Place of Presentation: 大阪国際会議場（大阪市北区）
  • Year and Date: 2016-04-15
• [Presentation] The detection of colorectal CTC by the use of polymeric microfluidic devices (2015)
  • Author(s): 呉一眞，細谷理樹，小見山博光，冨木裕一，落合 匠，大永崇，坂本一博
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-08
• [Presentation] Patient-derived colon carcinoma cells disseminate into distant organs in various different fashionｓ (2015)
  • Author(s): Okazawa Y, Mizukoshi K, Komiyama H, Fujii T, Goto M, Hino O, Sakamoto K, Orimo A
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-08
• [Presentation] Metastatic seeding of patient-derived colon cancer cells maintaining epithelial or mesenchymal trait (2015)
  • Author(s): 水越 幸輔，岡澤 裕 ，門脇奈穂美，小見山博光，小島 豊，五藤 倫敏，垣生 園子，樋野 興夫，折茂 彰 ，坂本 一博
  • Organizer: 第70回日本消化器外科学会総会
  • Place of Presentation: 浜松
  • Year and Date: 2015-07-15
• [Presentation] Comparisons of long-term outcomes for locally advanced colon cancer between laparoscopic colectomy and open colectomy; propensity score analysis (2015)
  • Author(s): Sugimoto K, Sakamoto K, Tomiki Y, Goto M, Kojima Y, Komiyama H, Takahashi M, Kamiyama H, Ishiyama S, Niwa K, Ito S, Kawai M, Takehara K, Tashiro T, Munakata S, Aoki J, Okazawa Yu O, Takahashi R, Mizukoshi K, Ro H, Ichikawa R, Kure K, Honjyo K, Tsukamoto R.
  • Organizer: 23th International Congress of the EAES
  • Place of Presentation: Bucharest, Romania
  • Year and Date: 2015-06-03
  • Int'l Joint Research
• [Presentation] CHFR methylation is an independent prognostic marker of poor prognosis in gastric cancer (2015)
  • Author(s): Sugimoto K, Humbert A, Lu Z, Chen C, Rodgers K, Lee,B Griffin C, Sato K, Orita H, Ito T, Komiyama H, Kojima Y, Goto M, Tomiki Y, Sakamoto K, Brock M
  • Organizer: 2015 American Association for Cancer Research Annual Meeting
  • Place of Presentation: Pennsylvania, USA
  • Year and Date: 2015-04-18
  • Int'l Joint Research
• [Presentation] Comparative Genomic Hybridization (CGH) を用いた 大腸がんの肝転移と予後因子の検討 (2014)
  • Author(s): 河合雅也，小見山博光，呉 一眞，本庄薫平，盧 尚志,
  • Organizer: 2014 JDDW 第56回日本消化器病学会大会
  • Place of Presentation: 神戸,神戸市ポートピアアイランド
  • Year and Date: 2014-10-23 – 2014-10-26
• [Presentation] 大腸癌における血清p53抗体の検討 (2014)
  • Author(s): 高橋里奈，呉 一眞，本庄薫平，盧 尚志，青木 順，岡澤 裕，，水越幸輔，河合雅也，嵩原一裕，宗像慎也，石山 隼，杉本起一，小見山博光，高橋 玄，柳沼行宏，小島 豊，五藤倫敏，奥澤淳司，，冨木裕一，坂本一博
  • Organizer: 2014 JDDW 第56回日本消化器病学会大会
  • Place of Presentation: 神戸,神戸市ポートピアアイランド
  • Year and Date: 2014-10-23 – 2014-10-26
• [Presentation] Visceral fat area and body mass index in laparoscopic surgery for colorectal cancer (2014)
  • Author(s): Yutaka Kojima, Kazuhiro Sakamoto, Yuichi Tomiki, Atsushi Okuzawa, Michitoshi Goto, Hiromitsu Komiyama, Makoto Takahashi, Yukihiro Yaginuma, Hirohiko Kamiyama
  • Organizer: The 14th World Congress of Endoscopic Surgery
  • Place of Presentation: Paris
  • Year and Date: 2014-06-25 – 2014-06-28
• [Presentation] Experience of using fine forceps in laparoscopic surgery for colorectal cancer (2014)
  • Author(s): Rina TAKAHASHI, Kazuhiro Sakamoto, Yuichi Tomiki, Atsushi Okuzawa, Michitoshi Goto,Yutaka Kojima, Hiromitsu Komiyama, Makoto Takahashi, Yukihiro Yaginuma, Hirohiko Kamiyama
  • Organizer: The 14th World Congress of Endoscopic Surgery
  • Place of Presentation: Paris
  • Year and Date: 2014-06-25 – 2014-06-28
• [Presentation] EXPERIENCE OF SINGLE-INCISION LAPAROSCOPIC SURGERY FOR THE SMALL INTESTINE LESION (2014)
  • Author(s): Shingo Ito，Kazuhiro Sakamoto，Yuichi Tomiki，Atsushi Okuzawa，Michitoshi Goto，Yutaka Kojima，Hiromitsu Komiyama，Makoto Takahashi，Yukihiro Yaginuma
  • Organizer: The Society of American Gastrointestinal and Endoscopic Surgeons
  • Place of Presentation: Salt Lake City
  • Year and Date: 2014-04-02 – 2014-04-05
• [Remarks] 順天堂大学大学院 下部消化管外科学
  • URL: https://www.juntendo.ac.jp/graduate/laboratory/labo/kabusyoukakan/
• [Remarks] 順天堂大学 下部消化管外科
  • URL: http://www.juntendo.ac.jp/graduate/laboratory/labo/kabusyoukakan/