• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Optimized magnitude of cryosurgery facilitating anti‑tumor immunoreaction in a mouse model of Lewis lung cancer

Research Project

Project/Area Number 26462137
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Respiratory surgery
Research InstitutionTeikyo University

Principal Investigator

Masafumi Kawamura  帝京大学, 医学部, 教授 (70169770)

Co-Investigator(Kenkyū-buntansha) 高橋 祐介  帝京大学, 医学部, 助教 (00445214)
松谷 哲行  帝京大学, 医学部, 准教授 (60621943)
泉 陽太郎  埼玉医科大学, 医学部, 講師 (90245506)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords肺癌 / 腫瘍免疫 / クライオセラピー / 凍結壊死融解療法 / サイトカイン / 腫瘍浸潤T細胞 / 凍結療法 / 免疫反応
Outline of Final Research Achievements

Cryosurgery, consisted of 60 sec freezing with liquid nitrogen and 60 sec sawing cycle, was performed to one tumor of bilateral femoral tumors that originated from Lewis Lung Cancer cell. Histologically, there was a significant correlation between the number of cycles of cryosurgery and the area of nonviable tumor area. 75% of the nonviable tumor area was observed in the 2 cycle group, and the abscopal tumor growth rate was significantly slower than that in the other groups. In immunohistochmistry, both the CD4 and CD8 positive T cells infiltration was significantly increased and Ki - 67 positive tumor cells were significantly decreased in the 2 cycle group . There was a significant increase in IL-1β, IL-2, IL-6, IL-12, and TNF-α in the 2-cycle group as compared with the other groups. Our data suggest that infiltration of both CD4 positive and CD8 positive T cells into the abscopal tumor via the increased proinflammatory cytokines could suppress the tumor growth.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (8 results)

All 2016 2015

All Journal Article (8 results) (of which Peer Reviewed: 8 results,  Acknowledgement Compliant: 3 results,  Open Access: 2 results)

  • [Journal Article] Optimized magnitude of cryosurgery facilitating anti-tumor immunoreaction in a mouse model of Lewis lung cancer2016

    • Author(s)
      Takahashi Y, Izumi Y, Matsutani N, Dejima H, Nakayama T, Okamura R, Uehara H, Kawamura M.
    • Journal Title

      Cancer Immunol Immunother

      Volume: 65 Issue: 8 Pages: 973

    • DOI

      10.1007/s00262-016-1858-x

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Therapeutic potential of recombinant thrombomodulin for lung injury after pneumonectomy via inhibition of high-mobility group box 1 in mice2016

    • Author(s)
      Takahashi Y, Matsutani N, Dejima H, Nakayama T, Okamura R, Uehara H, Kawamura M.
    • Journal Title

      J Trauma Acute Care Surg

      Volume: 81 Issue: 5 Pages: 868

    • DOI

      10.1097/ta.0000000000001208

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Mediastinal hemorrhage due to ectopic parathyroid hyperplasia with long-term hemodialysis: report of a case2015

    • Author(s)
      Matsutani N, Kawamura M, et al.
    • Journal Title

      Surg Today

      Volume: 45 Issue: 12 Pages: 1572

    • DOI

      10.1007/s00595-015-1122-6

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Predictors of post-recurrence survival in patients with non-small-cell lung cancer initially completely resected2015

    • Author(s)
      Takahashi Y, Horio H, et al.
    • Journal Title

      Int Cardiovasc Thorac Surg

      Volume: 21 Issue: 1 Pages: 14

    • DOI

      10.1093/icvts/ivv085

    • Related Report
      2015 Research-status Report 2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Prognostic Significance of Preoperative Neutrophil–Lymphocyte Ratios in Patients with Stage I Non-small Cell Lung Cancer After Complete Resection2015

    • Author(s)
      Takahashi Y, Horio H, et a.l
    • Journal Title

      Ann Surg Oncol

      Volume: 22 Issue: S3 Pages: 1324

    • DOI

      10.1245/s10434-015-4735-5

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Neutrophil-Lymphocyte Ratio as a Prognostic Marker for Lung Adenocarcinoma after Complete Resection2015

    • Author(s)
      Takahashi Y, Kawamura M, et al.
    • Journal Title

      W J Surg

      Volume: 40 Issue: 2 Pages: 365

    • DOI

      10.1007/s00268-015-3275-2

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Qualitative analysis of preoperative high-resolution computed tomography: Risk factors for pulmonary complications after major lung resection2015

    • Author(s)
      Takahashi Y, Matsuda M, et al.
    • Journal Title

      Ann Thorac Surg

      Volume: 101 Issue: 3 Pages: 1068

    • DOI

      10.1016/j.athoracsur.2015.09.009

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Significant correlation between urinary N1, N12-diacetylspermine and tumor invasiveness in patients with clinical stage IA non-small cell lung cancer.2015

    • Author(s)
      Takahashi Y, Horio H, et al.
    • Journal Title

      BMC Cancer

      Volume: 15 Issue: 1 Pages: 65-65

    • DOI

      10.1186/s12885-015-1068-5

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi