Establishment of cerebral embolic stroke treatment by Staphylokinase

• Project/Area Number: 26462149
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurosurgery
• Research Institution: Hirosaki University
• Principal Investigator: Shimamura Norihito 弘前大学, 医学研究科, 講師 (40312491)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: Staphylokinase / Cerebral infarction / Embolic stroke / スタフィロキナーゼ / 脳塞栓症 / 脳梗塞 / 脳浮腫 / 脳卒中

Outline of Final Research Achievements

Four hundred microgram per kg Staphylokinase (SAK) reduced cerebral infarction same as clinical dose of t-PA. The rate of respiratory trouble and mortality in SAK groups were significantly smaller than t-PA group. Reduction of MMP-9 and preservation of blood brain barrier were detected. And intra-arterial injection of VEGF antagonist significantly reduced cerebral infarction volume and preserve neural function. Reduction of IP3KA and Occludin were detected. High dose SAK is an alternative to t-PA in the treatment of embolic ischemic stroke. Combination therapy of SAK and VEGF antagonist achieved synergism in ischemic stroke treatment.

Research Products

• [Journal Article] Neuro-regeneration therapy using human Muse cells is highly effective in a mouse intracerebral hemorrhage model (2017)
  • Author(s): Shimamura N., Kakuta K., Wang L., Naraoka M., Uchida H., Wakao S., Dezawa M. Ohkuma H.
  • Journal Title: Exp Brain Res Volume: 235 Issue: 2 Pages: 565-572
  • DOI: 10.1007/s00221-016-4818-y
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Stem cell therapies for intracerebral hemorrhages. (2017)
  • Author(s): Shimamura N, Matsuda N, Katayama K, Kakuta K, Katagai T, Yamanaka H, Ohkuma H.
  • Journal Title: Current Drug Delivery Volume: 13 Issue: 6
  • DOI: 10.2174/1567201813666160922115830
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Analysis of factors that influence long-term independent living for elderly subarachnoid hemor rhage patients (2016)
  • Author(s): Shimamura N, Naraoka M, Katagai T, Katayama K, Matsuda N, Ohkuma H
  • Journal Title: World Neurosurgery Volume: ー Pages: 504-510
  • DOI: 10.1016/j.wneu.2016.03.057
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Epidemiological Investigation of Patients in Persistent Vegetative States in Aomori, Japan (2015)
  • Author(s): Shimamura N, Munakata S, Naraoka M, Ogasawara Y, Kanae O, Ohkuma H
  • Journal Title: Neurological Surgery 脳神経外科 Volume: 43 Issue: 8 Pages: 705-708
  • DOI: 10.11477/mf.1436203106
  • ISSN: 0301-2603, 1882-1251
  • Year and Date: 2015-08-10
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Neuro-regeneration therapy for a mouse ICH model using a multi-lineage differentiating stress enduring cell (2016)
  • Author(s): Shimamura N
  • Organizer: 2016 AANS Annual Scientific Meeting
  • Place of Presentation: シカゴ、米国
  • Year and Date: 2016-04-30
  • Int'l Joint Research
• [Presentation] Staphylokinase ameliorate embolic cerebral infarction in rat model (2016)
  • Author(s): Shimamura N, Kakuta K, Katayama K, Matsuda N, Ogasawara Y, Oyama K, Ohkuma H
  • Organizer: The 3rd International Conference on Heart & Brain
  • Place of Presentation: パリ（フランス）
  • Year and Date: 2016-02-25
  • Int'l Joint Research
• [Presentation] Staphylokinase ameliorate embolic cerebral infarction in the rat model (2015)
  • Author(s): 嶋村 則人
  • Organizer: 24th European Stroke Conference
  • Place of Presentation: ウィーン
  • Year and Date: 2015-05-13 – 2015-05-15
• [Presentation] Neutralizing VEGF via the internal carotid artery reduces brain edema and improve neurological function in rat transient MCAO model (2015)
  • Author(s): Shimamura N, Matsuda N, Ogasawara Y, Oyama K, Ohkuma H
  • Organizer: XXIV European Stroke Conference
  • Place of Presentation: ウィーン（オーストリア）
  • Year and Date: 2015-05-13
  • Int'l Joint Research
• [Remarks] 弘前大学大学院医学研究科脳神経外科学講座
  • URL: http://www.med.hirosaki-u.ac.jp/~neuros/
• [Remarks] 弘前大学大学院医学研究科脳神経外科
  • URL: http://www.med.hirosaki-u.ac.jp/~neuros/