| Project/Area Number |
26462158
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurosurgery
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| Research Institution | Kobe University |
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Principal Investigator |
Mizukawa Katsu 神戸大学, 医学部附属病院, 非常勤講師 (80403260)
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| Co-Investigator(Kenkyū-buntansha) |
篠山 隆司 神戸大学, 医学部附属病院, 講師 (10379399)
田中 一寛 神戸大学, 医学研究科, 医学研究員 (70467661)
細田 弘吉 神戸大学, 医学研究科, 准教授 (90403261)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | cerebral infarction / microRNA / metabolome / 脳梗塞 / マイクロRNA / 代謝 / アポトーシス / microRNA-21 / apoptosis / 神経細胞死 |
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| Outline of Final Research Achievements |
In the mouse brain of cerebral ischemia (120-min middle cerebral artery occlusion (MCAO)), microRNA 21 (miR-21) expression was significantly increased, although miR-21 was not increased in 30-min MCAO brain. next, we performed a comparative metabolic analysis of the effects of cerebral ischemia on the metabolism. Metabolic profiling by gas-chromatography/mass-spectrometry analysis showed clear separation between the ischemia and control group. citrate, GABA, Grycerol, and Glysine etc. were significantly increased in MCAO brain, on the other hand, Glutamate, Ribrose-5P, Fructose-6P, Pyrvate, Glucose, etc. were significantly decreased in MCAO brain. The decreases of fructose 6-phosphate and ribulose 5-phosphate suggested enhancement of the pentose phosphate pathway (PPP) during cerebral ischemia.
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