Effect of microRNA -21 in cerebral ischemia.

• Project/Area Number: 26462158
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurosurgery
• Research Institution: Kobe University
• Principal Investigator: Mizukawa Katsu 神戸大学, 医学部附属病院, 非常勤講師 (80403260)
• Co-Investigator(Kenkyū-buntansha): 篠山 隆司 神戸大学, 医学部附属病院, 講師 (10379399) ; 田中 一寛 神戸大学, 医学研究科, 医学研究員 (70467661) ; 細田 弘吉 神戸大学, 医学研究科, 准教授 (90403261)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: cerebral infarction / microRNA / metabolome / 脳梗塞 / マイクロRNA / 代謝 / アポトーシス / microRNA-21 / apoptosis / 神経細胞死

Outline of Final Research Achievements

In the mouse brain of cerebral ischemia (120-min middle cerebral artery occlusion (MCAO)), microRNA 21 (miR-21) expression was significantly increased, although miR-21 was not increased in 30-min MCAO brain. next, we performed a comparative metabolic analysis of the effects of cerebral ischemia on the metabolism. Metabolic profiling by gas-chromatography/mass-spectrometry analysis showed clear separation between the ischemia and control group. citrate, GABA, Grycerol, and Glysine etc. were significantly increased in MCAO brain, on the other hand, Glutamate, Ribrose-5P, Fructose-6P, Pyrvate, Glucose, etc. were significantly decreased in MCAO brain. The decreases of fructose 6-phosphate and ribulose 5-phosphate suggested enhancement of the pentose phosphate pathway (PPP) during cerebral ischemia.

Research Products

• [Journal Article] Cisterna magna meningiomas without dural attachment: Report of two cases. (2017)
  • Author(s): Kohta M, Sasayama T, Nakai T, Taniguchi M, Nagashima H, Tanaka K, Mizukawa K, Itoh T, Kohmura E.
  • Journal Title: Neurol Neurochir Po Volume: epub Pages: 1-5
  • Peer Reviewed
• [Journal Article] Diagnostic value of glutamate with 2-hydroxyglutarate in magnetic resonance spectroscopy for IDH1 mutant glioma (2016)
  • Author(s): Nagashima H, Tanaka K, Sasayama T, Irino Y, Sato N, Takeuchi Y, Kyotani K, Mukasa A, Mizukawa K, Sakata J, Yamamoto Y, Hosoda K, Itoh T, Sasaki R, Kohmura E.
  • Journal Title: Neuro Oncol Volume: 18(11) Pages: 1559-1568
  • DOI: 10.1093/neuonc/now090
  • Peer Reviewed
• [Journal Article] Radiographic occult cerebellar germinoma presenting with progressive ataxia and cranial nerve palsy. (2016)
  • Author(s): Minami N, Tanaka K, Kimura H, Hirose T, Mori T, Maeyama M, Sekiya H, Uenaka T, Nakamizo S, Nagashima H, Mizukawa K, Itoh T, Sasayama T, Kohmura E.
  • Journal Title: BMC Neurol. Volume: 16 Issue: 1 Pages: 1-4
  • DOI: 10.1186/s12883-015-0516-9
  • Peer Reviewed / Open Access
• [Journal Article] Endoscopic endonasal translacerum approach to the inferior petrous apex. (2016)
  • Author(s): Taniguchi M, Akutsu N, Mizukawa K, Kohta M, Kimura H, Kohmura E.
  • Journal Title: J Neurosurg Volume: 124 Pages: 1032-8
  • Peer Reviewed
• [Journal Article] Two Cases of Cerebral Infarction due to Focal Irradiation for Glioma in Adults (2015)
  • Author(s): Minami N, Mizukawa K, Iwahashi H, Nagashima H, Tanaka K, Sasayama T, Hosoda K, Mukumoto N, Sasaki R, Kohmura E.
  • Journal Title: Neurological Surgery 脳神経外科 Volume: 43 Issue: 4 Pages: 344-351
  • DOI: 10.11477/mf.1436203018
  • ISSN: 0301-2603, 1882-1251
  • Year and Date: 2015-04-10
  • Peer Reviewed
• [Journal Article] Tumor-Associated Macrophages Associate with Cerebrospinal Fluid Interleukin-10 and Survival in Primary Central Nervous System Lymphoma (PCNSL) (2015)
  • Author(s): Sasayama T, Tanaka K, Mizowaki T, Nagashima H, Nakamizo S, Tanaka H, Nishihara M, Mizukawa K, Hirose T, Itoh T, Kohmura E
  • Journal Title: Brain Pathol. Volume: Aug 28 Issue: 4 Pages: 1-9
  • DOI: 10.1111/bpa.12318
  • Peer Reviewed
• [Journal Article] Combined IDH1 mutation and MGMT methylation status on long-term survival of patients with cerebral low-grade glioma. (2015)
  • Author(s): Tanaka K, Sasayama T, Mizukawa K, Takata K, Sulaiman NS, Nishihara M, Kohta M, Sasaki R, Hirose T, Itoh T, Kohmura E.
  • Journal Title: Clin Neurol Neurosurg. Volume: 138 Pages: 37-44
  • DOI: 10.1016/j.clineuro.2015.07.019
  • Peer Reviewed / Open Access
• [Journal Article] A 40-Year-Old Female with Leptomeningeal Lesions. (2015)
  • Author(s): Mizowaki T, Sasayama T, Semba S, Sasaki R, Yasuo K, Nakamizo S, Tanaka K, Mizukawa K, Uchihashi Y, Kohmura E.
  • Journal Title: Brain Pathol. Volume: 25 Issue: 4 Pages: 509-10
  • DOI: 10.1111/bpa.12270
  • Peer Reviewed / Open Access
• [Journal Article] STAT3 activation is associated with cerebrospinal fluid interleukin-10 (IL-10) in primary central nervous system diffuse large B cell lymphoma. (2015)
  • Author(s): Mizowaki T, Sasayama T, Tanaka K, Mizukawa K, Takata K, Nakamizo S, Tanaka H, Nagashima H, Nishihara M, Hirose T, Itoh T, Kohmura E
  • Journal Title: J Neurooncol Volume: 124(2) Issue: 2 Pages: 165-74
  • DOI: 10.1007/s11060-015-1843-9
  • Peer Reviewed
• [Journal Article] Compensatory glutamine metabolism promotes glioblastoma resistance to mTOR inhibitor treatment. (2015)
  • Author(s): Tanaka K, Sasayama T, Irino Y, Takata K, Nagashima H, Satoh N, Kyotani K, Mizowaki T, Imahori T, Ejima Y, Masui K, Gini B, Yang H, Hosoda K, Sasaki R, Mischel PS, Kohmura E.
  • Journal Title: J Clin Invest Volume: 125 Issue: 4 Pages: 1591-602
  • DOI: 10.1172/jci78239
  • Peer Reviewed / Open Access
• [Journal Article] 2.Different spatial distribution between germinal center B and non-germinal center B primary central nervous system lymphoma revealed by magnetic resonance group analysis. (2014)
  • Author(s): Kinoshita M1, Sasayama T, Narita Y, Yamashita F, Kawaguchi A, Chiba Y, Kagawa N, Tanaka K, Kohmura E, Arita H, Okita Y, Ohno M, Miyakita Y, Shibui S, Hashimoto N, Yoshimine T
  • Journal Title: Neuro Oncol Volume: 16 Issue: 5 Pages: 728-734
  • DOI: 10.1093/neuonc/not319
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 当施設でのパーキンソン病に対する脳深部刺激療法 (2016)
  • Author(s): 水川 克、近藤 威、内橋義人、鵜山 淳、森下暁二、甲村英二
  • Organizer: 日本脳神経外科学会 第75回学術総会
  • Place of Presentation: 福岡
• [Presentation] 膠芽腫再発に対して追加放射線治療とBevacizumab（アバスチン）療法を行った３例 (2015)
  • Author(s): 水川克、前山昌博、藤本陽介、田中一寛、篠山隆司、佐々木良平、西村英輝、藤井正彦、甲村英二
  • Organizer: 第33回日本脳腫瘍学会
  • Place of Presentation: 京都
  • Year and Date: 2015-12-06
• [Presentation] 当施設でのバクロフェン髄注療法について (2015)
  • Author(s): 水川克、甲村英二
  • Organizer: 第74回日本脳神経外科学会学術総会
  • Place of Presentation: 札幌
  • Year and Date: 2015-10-14
• [Presentation] 脳内出血を来たした滑膜肉腫の脳転移の１例 (2014)
  • Author(s): 水川 克、堀 達雄、森 達也、鵜山 淳、南 徳明、長嶋宏明、田中一寛、篠山隆司、細田弘吉、甲村英二
  • Organizer: 第32回日本脳腫瘍学会
  • Place of Presentation: 東京
  • Year and Date: 2014-11-30 – 2014-12-02
• [Presentation] 当院における悪性神経膠腫再発例に対するアバスチン(Bevacizumab)療法 (2014)
  • Author(s): 水川克、篠山隆司、田中一寛、長嶋宏明、堀達雄、細田弘吉、甲村英二
  • Organizer: 第73回日本脳神経外科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2014-10-09 – 2014-10-11
• [Presentation] Characteristics of microRNA expressions in glioblastoma with metastases to the medulla oblongata or spinal cord. (2014)
  • Author(s): Takashi Sasayama, Nakada Mitsutoshi, Kazuhiro Tanaka, Takashi Mizowaki, Masamitsu Nishihara, Katsu Mizukawa, Satoshi Nakamizo, Hirotomo Tanaka, Masaaki Kohta, Eiji Kohmura
  • Organizer: 11th Meeting of the Asian Society for Neuro-Oncology
  • Place of Presentation: Istanbul
  • Year and Date: 2014-09-11 – 2014-09-14
• [Presentation] グリオーマにおける脳脊髄液を用いたメタボローム解析 (2014)
  • Author(s): 篠山隆司、中溝聡、篠原正和、入野康弘、西海信、田中宏知、田中一寛、水川克、細田弘吉、吉田優、甲村英二
  • Organizer: 第2回がんと代謝研究会
  • Place of Presentation: 東京
  • Year and Date: 2014-07-10 – 2014-07-11
• [Book] Primary Central Nervous System Lymphoma (PCNSL): Incidence, Management and Outcomes (2016)
  • Author(s): Takashi Sasayama, Kazuhiro Tanaka, Katsu Mizukawa, Masamitsu Nishihara, and Eiji Kohmura
  • Total Pages: 310
  • Publisher: NOVA SCIENCE PUBLISHERS, INC
• [Book] Primary Central Nervous System Lymphoma (PCNSL): Incidence, Management and Outcomes: Cerebrospinal Fluid Interleukin-10 (IL-10) As a Diagnostic Marker in Primary Central Nervous System Lymphoma (2016)
  • Author(s): Takashi Sasayama, Kazuhiro Tanaka, Katsu Mizukawa, Masamitsu Nishihara and Eiji Kohmura
  • Total Pages: 16
  • Publisher: NOVA SCIENTIFIC PUBLISHER INC.
• [Book] 神経症候群（第2版）III－その他の神経疾患を含めて－ (2014)
  • Author(s): 水澤英洋、渋井壮一郎、西川 亮、篠山隆司ほか
  • Total Pages: 878
  • Publisher: 日本臨床