Therapy for cerebral infarction using infiltrated cells in ischemic brain
Project/Area Number |
26462162
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Neurosurgery
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Research Institution | Ehime University |
Principal Investigator |
Kumon Yoshiaki 愛媛大学, 医学系研究科, 寄附講座教授 (80127894)
|
Co-Investigator(Kenkyū-buntansha) |
渡邉 英昭 愛媛大学, 医学系研究科, 講師 (30322275)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | 脳梗塞 / マクロファージ / CD200 / NG2 / 神経栄養因子 |
Outline of Final Research Achievements |
Two types of macrophages in lesion core of rat stroke model were identified according to NG2 chondroitin sulfate proteoglycan (NG2) and CD200 expression. NG2+ macrophages were CD200-, and vice versa. Although CD200+ macrophages cannot be classified as either M1 or M2, CD200+ macrophages expressed two splice variants of CD200 that are CD200L and CD200S. Rats transplanted with C6-CD200S cells in the brain survived for a longer period than those transplanted with original C6 or C6-CD200L cells. The C6-CD200S tumors were smaller than the C6-CD200L or C6-original tumors, and many apoptotic cells were found in the tumor cell aggregates. Tumor-associated macrophages in C6-CD200S tumors displayed dendritic cell -like morphology and CD86 expression. These results suggested that the inflammatory reaction induced by CD200S is superior to the contrary function by CD200L in the ischemic brain.
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Report
(4 results)
Research Products
(8 results)
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[Journal Article] A truncated form of CD200(CD200S) expressed on glioma cells prolonged survival in a rat glioma model by induction of a dendritic cell-like phenotype in tumor-associated macrophages2016
Author(s)
Kobayashi K, Yano H, Umakoshi A, Matsumoto S, Mise A, Funahashi Y, Ueno Y, Kamei Y, Takada Y, Kumon Y, Ohnishi T, Tanaka J
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Journal Title
Neoplasia
Volume: 18
Pages: 229-241
Related Report
Peer Reviewed
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[Journal Article] CD200+ and CD200- macrophages accumulated in ischemic lesions of rat brain: the two populations cannot be classified as either M1 or M2 macrophages.2015
Author(s)
Shirabe Matsumoto, Junya Tanaka, Hajime Yano, Hisaaki Takahashi, Kana Sugimoto, Shiro Ohue, Akihiro Inoue, Hitomi Aono, Akari Kusakawa, Hideaki Watanabe, Yoshiaki Kumon, Takanori Ohnishi
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Journal Title
Journal of Neuroimmunology
Volume: 15
Pages: 7-20
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] CD200+ and CD 200- macrophages accumulated in ischemic lesions of rat brain: The two populations cannot be classified as either M1 or M2 macrophages2015
Author(s)
Shirabe Matsumoto, Junya Tanaka, Hajime Yano, Hisaaki Takahashi, Kana Sugimoto, Shiro Ohue, Akihiro Inoue, Hitomi Aono, Akari Kusakawa, Hideaki Watanabe, Yoshiaki Kumon, Takanori Ohnishi
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Journal Title
Journal of Neuroimmunology
Volume: 282
Pages: 7-20
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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