| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Two types of macrophages in lesion core of rat stroke model were identified according to NG2 chondroitin sulfate proteoglycan (NG2) and CD200 expression. NG2+ macrophages were CD200-, and vice versa. Although CD200+ macrophages cannot be classified as either M1 or M2, CD200+ macrophages expressed two splice variants of CD200 that are CD200L and CD200S.
Rats transplanted with C6-CD200S cells in the brain survived for a longer period than those transplanted with original C6 or C6-CD200L cells. The C6-CD200S tumors were smaller than the C6-CD200L or C6-original tumors, and many apoptotic cells were found in the tumor cell aggregates. Tumor-associated macrophages in C6-CD200S tumors displayed dendritic cell -like morphology and CD86 expression. These results suggested that the inflammatory reaction induced by CD200S is superior to the contrary function by CD200L in the ischemic brain.
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