Effect of pericyte on the blood-brian barrier damage due to the hyperglycemia
Project/Area Number |
26462166
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Neurosurgery
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Research Institution | Nagasaki University |
Principal Investigator |
HAYASHI Kentaro 長崎大学, 医歯薬学総合研究科(医学系), 客員研究員 (40404222)
|
Co-Investigator(Kenkyū-buntansha) |
出雲 剛 長崎大学, 病院(医学系), 講師 (40343347)
諸藤 陽一 長崎大学, 病院(医学系), 助教 (40437869)
福田 修志 長崎大学, 病院(医学系), 医員 (40646577)
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Project Period (FY) |
2014-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | blood-brain barrier / Glucagon-like peptide-1 / 血液-脳関門 / ラット / 高血糖 / ペリサイト |
Outline of Final Research Achievements |
GLP-1 increased transendothelial electrical resistance and decreased the permeability of sodium fluorescein in RBECs in a dose- and time-dependent manner. The effects on these barrier functions were significantly reduced in the presence of the GLP-1 receptor antagonist exendin-3 (9-39) and the protein kinase A (PKA) inhibitor H-89. Western blot analysis showed that GLP-1 increased the amount of occludin and claudin-5. GLP-1 analogs are approved for treatment of type 2 diabetes mellitus, and thus, we examined the effects of GLP-1 on hyperglycemia-induced BBB damage. GLP-1 inhibited the increase in production of reactive oxygen species under hyperglycemia conditions and improved the BBB integrity induced by hyperglycemia. As GLP-1 stabilized the integrity of the BBB, probably via cAMP/PKA signaling, the possibility that GLP-1 acts as a BBB-protective drug should be considered.
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Report
(5 results)
Research Products
(43 results)
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[Journal Article] Point-by-point parent artery/sinus obliteration using detachable, pushable, 0.035-inch coils2016
Author(s)
Yamaguchi S, Horie N, Hayashi K, Fukuda S, Morofuji Y, Hiu T, Izumo T, Morikawa M, Matsuo T
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Journal Title
Acta Neurochir (Wien)
Volume: 158
Pages: 2089-2094
Related Report
Peer Reviewed
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