| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Ten plaques removed from carotid endarterectomy patients were classified into high- and low-calcified plaques on the basis of Agatston calcium score. Microarray analysis for miRNA profiles was performed followed by validation with miRNA quantitative real-time (qRT) PCR analysis. Genes which satisfied total gene signal (TGS) >50 and [Log2 ratio <-1 or >1 were selected. Among carefully selected 70 angiogenesis or calcification-related transcripts, reliably expressed miRNAs marking median and high context scores with miRSearch and FDR <0.1 by the two-group comparison were also extracted .In high-calcified carotid plaques, specific profile for miRNA may be shown and expression of hsa-miR-4530 and hsa-miR-133b had inverse correlation with calcium score in plaques, suggesting miRNA may play a modulating role in calcification in plaque and plaque stability.
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