• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Investigation of stability of calcified plaque by miRNA profiling

Research Project

Project/Area Number 26462169
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Neurosurgery
Research InstitutionNagoya City University

Principal Investigator

KATANO Hiroyuki  名古屋市立大学, 大学院医学研究科, 准教授 (30295612)

Co-Investigator(Kenkyū-buntansha) 山田 和雄  名古屋市立大学, 大学院医学研究科, 教授 (90150341)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordscarotid plaque / calcification / microRNA / microarray / PCR / carotid endarterectomy / calcium score / miRNA / RT-PCR
Outline of Final Research Achievements

Ten plaques removed from carotid endarterectomy patients were classified into high- and low-calcified plaques on the basis of Agatston calcium score. Microarray analysis for miRNA profiles was performed followed by validation with miRNA quantitative real-time (qRT) PCR analysis. Genes which satisfied total gene signal (TGS) >50 and [Log2 ratio <-1 or >1 were selected. Among carefully selected 70 angiogenesis or calcification-related transcripts, reliably expressed miRNAs marking median and high context scores with miRSearch and FDR <0.1 by the two-group comparison were also extracted .In high-calcified carotid plaques, specific profile for miRNA may be shown and expression of hsa-miR-4530 and hsa-miR-133b had inverse correlation with calcium score in plaques, suggesting miRNA may play a modulating role in calcification in plaque and plaque stability.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (2 results)

All 2017 2015

All Presentation (2 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] 石灰化粥腫とmicroRNA発現2017

    • Author(s)
      片野広之、西川祐介、山田紘史、間瀬光人
    • Organizer
      第46回日本脳卒中の外科学会
    • Place of Presentation
      大阪
    • Year and Date
      2017-03-18
    • Related Report
      2016 Annual Research Report
  • [Presentation] Preoperative in silico analysis of atherosclerotic calcification vulnerability in carotid artery stenting using Finite Element Analysis by considering Agatston score2015

    • Author(s)
      Riyahi-Alam S, Morbiducci U, Katano H, Yokoyama K, Ali S, Audenino A, Molinari F
    • Organizer
      IUPESM World Congress on Medical Physics and Biomedical Engineering
    • Place of Presentation
      Toronto, CANADA
    • Year and Date
      2015-06-07
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi