| Project/Area Number |
26462170
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurosurgery
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| Research Institution | Nara Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
三島 健一 福岡大学, 薬学部, 教授 (00320309)
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| Co-Investigator(Renkei-kenkyūsha) |
TAOKA Toshiaki 名古屋大学, 医学部, 准教授 (30305734)
ABE Kohji 大阪大学, 医学部, 特任准教授 (80571207)
NAKASE Hiroyuki 奈良県立医科大学, 医学部, 教授 (10217739)
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| Research Collaborator |
MUROI Carl, Izumi
FUJIMURA Yoshihiro
TSUBOI Akio
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | ADAMTS13 / クモ膜下出血 / 脳虚血 / 脳動脈瘤 / 血栓 / 炎症 / VWF / MRI / 遅発性脳虚血 / 動物モデル |
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| Outline of Final Research Achievements |
ADAMTS13 cleaves VWF and reduces its prothrombotic and proinflammatory functions. Thus, ADAMTS13 protects the brain against acute ischemia by ameliorating post-ischemic brain hypoperfusion and inflammation. We studied the effect of ADAMTS13 on delayed cerebral ischemia after aneurysmal SAH. We evaluated the mice SAH model with 7.0 Tesla MRI and histology. The effects of ADAMTS13 on delayed cerebral microthrombi, cerebral vasospasm, neuronal inflammation, and neuronal death in the cerebral cortex and hippocampus were analyzed over time after SAH by comparing the groups with and without ADAMTS13 administration. ADAMTS13 significantly reduced microthrombosis, neuroinflammation and degenerative neurons, and improved neurological performance in SAH models. Our study with ADAMTS13 knockout mice signified neuroprotective effects of ADAMTS13 in SAH models. ADAMTS13 could provide a novel therapeutic approach for delayed cerebral ischemia after SAH as well as for acute ischemic stroke.
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