The combination therapy of novel oncolytic virus with molecular targeted drug
Project/Area Number |
26462182
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Neurosurgery
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Research Institution | Okayama University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
安原 隆雄 岡山大学, 医歯薬学総合研究科, 講師 (50457214)
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Co-Investigator(Renkei-kenkyūsha) |
DATE Isao 岡山大学, 医歯薬学総合研究科, 教授 (70236785)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | Oncolytic virus / stem cell / antiangiogenesis / glioma / molecular targeted drug / antiinvasion |
Outline of Final Research Achievements |
Oncolytic viral (OV) therapy has been considered as a promising treatment modality for brain tumors. Previously a novel HSV-1 derived OV, 34.5ENVE (viral ICP34.5 Expressed by Nestin promotor and Vstat120 Expressing), was reported to have a potent antitumor effect. Here, we investigated the therapeutic efficacy of 34.5ENVE and cilengitide, an integrin inhibitor, or, bevacizumab, an VEGF antibody combination therapy for malignant glioma. Before the experiment of the combination with 34.5ENVE and targeted drug we evaluated the combination therapy with a first generation OV armed with the antiangiogenic gene Vstat120 (RAMBO). In the scratch wound assay, RAMBO CM significantly reduced both the number of migrating cells and the rate of migration. Furthermore, the number and the rate of migrating cells induced by bevacizumab treatment was reduced by RAMBO CM. These results indicate that targeted drug enhanced OV therapy for malignant glioma.
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Report
(4 results)
Research Products
(176 results)
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[Journal Article] Induction of WNT11 by hypoxia and hypoxia-inducible factor-1α regulates cell proliferation2016
Author(s)
Mori H, Yao Y, Learman BS, Kurozumi K, Ishida J, Ramakrishnan SK, Overmyer KA,Xue X, Cawthorn WP, Reid MA, Taylor M, Ning X, Shah YM, MacDougald OA
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Journal Title
Sci Rep
Volume: Feb 10
Issue: 1
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Serine/Threonine kinase MLK4 determines Mesenchymal Identity in Glioma Stem Cells in an NFkB-dependent manner2016
Author(s)
Kim SH, Ezhilarasan R, Chhipa R, Ladner K, Phillips E, Sparks A, Taylor D, Furuta T, Sabit H, Kurozumi K, Kuroiwa T, Akio A, Gallego-Perez1 D, Sulman EP, Cheng S, Lee J, Nakada M, Guttridge D, DasGupta B, Goidts V, Bhat KP, Walker J, Nakano I.
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Journal Title
Cancer Cell
Volume: 29
Issue: 2
Pages: 201-213
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Interaction of cytokeratin 19 head domain and HER2 in the cytoplasm leads to activation of HER2-Erk pathway.2016
Author(s)
Ohtsuka T, Sakaguchi M, Yamamoto H, Tomida S, Takata K, Shien K, Hashida S, Miyata-Takata T, Watanabe M, Suzawa K, Soh J, Youyi C, Sato H, Namba K, Torigoe H, Tsukuda K, Yoshino T, Miyoshi S, Toyooka S.
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Journal Title
Sci Rep
Volume: 6
Issue: 1
Pages: 33319-33319
DOI
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Peer Reviewed / Open Access
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[Journal Article] Annexin A2 regulates angiogenesis and invasion phenotypes of malignant glioma2015
Author(s)
Onishi M , Ichikawa T , Kurozumi K , Inoue S , Maruo T , Otani Y , Fujii K , Ishida J , Shimazu Y , Yoshida K , Michiue H , Chiocca EA , Date I
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Journal Title
Brain Tumor Patholog
Volume: -
Issue: 3
Pages: 184-194
DOI
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Peer Reviewed
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[Journal Article] Integrin antagonist augments the therapeutic effect of adenovirus-mediated REIC/Dkk-3 gene therapy for malignant glioma.2014
Author(s)
Shimazu Y, Kurozumi K, Ichikawa T, Fujii K, Onishi M, Ishida J, Oka T, Watanabe M, Nasu Y, Kumon H, Date I.
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Journal Title
Gene Ther
Volume: 22
Issue: 2
Pages: 146-154
DOI
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Peer Reviewed
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[Journal Article] Reduced neurotoxicity with combined treatment of high-dose methotrexate, cyclophosphamide, doxorubicin, vincristine and prednisolone (M-CHOP) and deferred radiotherapy for primary central nervous system lymphoma.2014
Author(s)
Ichikawa T, Kurozumi K, Michiue H, Ishida J, Maeda Y, Kondo E, Kawasaki A, Date I.
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Journal Title
Clin Neurol Neurosurg
Volume: 127
Pages: 106-11
DOI
Related Report
Peer Reviewed
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[Presentation] 視床グリオーマの治療成績2016
Author(s)
黒住和彦、亀田雅博、石田穣治、市川智継、伊達 勲
Organizer
第34回日本脳腫瘍学会学術集会
Place of Presentation
甲府富士屋ホテル(甲府市)
Year and Date
2016-12-05
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[Presentation] A super gene expression system enhances the anti-glioma effects of adenovirus-mediated REIC/Dkk-3 gene therapy2016
Author(s)
Oka T , Kurozumi K , Shimazu Y , Ichikawa T , Ishida J , Otani Y , Shimizu T , Tomita Y , Sakaguchi M , Watanabe M , Nasu Y , Kumon H , Date I
Organizer
第22回日本遺伝子細胞治療学会
Place of Presentation
虎ノ門ヒルズフォーラム(東京都港区)
Year and Date
2016-07-29
Related Report
Int'l Joint Research
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[Presentation] A super gene expression system enhances the antitumor effects of adenovirus -Mediated REIC/Dkk-3 gene therapy2016
Author(s)
Kurozumi K , Oka T , Shimazu Y , Ichikawa T , Shimizu T , Ishida J , Otani Y , Sakaguchi M , Watanabe M , Nasu Y , Kumon H , Date I
Organizer
21st International Conference on Brain Tumor Research and Therapy
Place of Presentation
万国津梁館(名護市)
Year and Date
2016-04-11
Related Report
Int'l Joint Research
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[Presentation] A super gene expression system enhances the anti-glioma effects of adenovirus-mediated REIC/Dkk-3 gene therapy2015
Author(s)
Oka T , Kurozumi K , Ichikawa T , Shimazu Y , Otani Y , Shimizu T , Sakaguchi M , Watanabe M , Nasu Y , Kumon H , Date I
Organizer
第21回日本遺伝子治療学会学術集会
Place of Presentation
大阪国際会議場(大阪)
Year and Date
2015-07-25
Related Report
Int'l Joint Research
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[Presentation] High survival rate in pediatric medulloblastoma with postoperative combined chemotherapy and reduced craniospinal radiotherapy (English Session)2014
Author(s)
,Ichikawa T , Shimizu T , Kurozumi K , Otani Y , Shimada A , Washio K , Nishiuchi R , Oda M , Katsui K , Yanai H , Date I
Organizer
第56回日本小児血液・がん学会学術集会
Place of Presentation
岡山
Year and Date
2014-11-28
Related Report
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[Presentation] Angiogenesis/invasion phenotypic shift regulated by Annexin A2 in malignant glioma2014
Author(s)
Otani Y , Ichikawa T , Onishi M , Kurozumi K , Inoue S , Maruo T , Ishida J , Shimazu Y , Oka T, Shimizu T , Michiue H , Chiocca EA , Date I
Organizer
11th Meeting of Asian Society for Neuro-Oncology
Place of Presentation
Istanbul, Turkey
Year and Date
2014-09-11
Related Report
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[Presentation] High response rate to methotrexate-based multiagent chemotherapy (M-CHOP) for primary central nervous system lymphoma2014
Author(s)
Ichikawa T , Kurozumi K , Michiue H , Ishida J , Shimazu Y , Oka T , Otani Y , Shimizu T , Maeda Y, Kondo E, Kawasaki A , Date I
Organizer
11th Meeting of Asian Society for Neuro-Oncology
Place of Presentation
Istanbul, Turkey
Year and Date
2014-09-11
Related Report
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[Presentation] Integrin inhibitor suppresses bevacizumab-induced glioma invasion2014
Author(s)
Shimizu T, Ishida J, Onishi M, Kurozumi K, Ichikawa T, Fujii K, Shimazu Y, Oka T, Otani Y, Date I
Organizer
11th Meeting of Asian Society for Neuro-Oncology
Place of Presentation
Istanbul, Turkey
Year and Date
2014-09-11
Related Report
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