| Project/Area Number |
26462228
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Chiba University |
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Principal Investigator |
Orita Sumihisa 千葉大学, 大学院医学研究院, 助教 (60638310)
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| Co-Investigator(Kenkyū-buntansha) |
高橋 和久 千葉大学, 大学院医学研究院, 教授 (20179477)
山内 かづ代 千葉大学, 医学部附属病院, 特任助教 (30648069)
大鳥 精司 千葉大学, 大学院医学研究院, 教授 (40361430)
久保田 剛 千葉大学, 医学部附属病院, 医員 (50725695)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 多血小板血漿 / 新鮮凍結 / 骨癒合 / 組織修復 / 凍結乾燥多血小板血漿 / 骨癒合促進 |
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| Outline of Final Research Achievements |
Fresh platelet-rich plasma (PRP) accelerates bone union in rat model. However, fresh PRP has a short half-life. We suggested freeze-dried PRP (FD-PRP) prepared in advance and investigated its efficacy on bone union in vivo. Spinal posterolateral fusion (PLF) was performed on 8-week-old male Sprague-Dawley rats, showing that FD-PRP accelerated bone union comparable to fresh PRP and BMP (human recombinant bone morphogenetic protein 2) by remodeling the bone with thinner but more tangled trabecular bone with rigidity. Histologically, the trabecular bone had thinner and more branches in the FD-PRP. And the biomechanical strength was high comparable to autologous bone. In conclusion, FD-PRP provides bone union ability comparable to fresh PRP and BMP, and can be prepared in advance safely and at low cost.
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