|Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|Outline of Final Research Achievements
In this study, we succeeded in establishing the novel mouse intervertebral disc degeneration model by surgically resecting lumbar facet joint, although we were not able to satisfactorily analyze the spine and intervertebral disc of the conditional FBN1 knockout mice because of delayed crossbleeding. By utilizing our novel mouse disc degeneration model and laser micro-digestion instrument, we analyzed the expression change of the FBN1 gene during the disc degeneration process and found the possible critical role of the FBN1 for the homeostasis in annulus fibrosus of the intervertebral disc. Also by utilizing the same model, we identified novel gene “X” which interacts with FBN1 in annulus fibrosus and promotes microfibril biogenesis in vitro. Now we are investigating the physiological functions of this new gene “X”, and exploring the possibility of this new gene as a novel drug for the disc degeneration.