• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The evaluation of protein expression and cyclic tensile strain of human OPLL

Research Project

Project/Area Number 26462235
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Orthopaedic surgery
Research InstitutionUniversity of Fukui

Principal Investigator

Sugita Daisuke  福井大学, 学術研究院医学系部門, 助教 (90596678)

Co-Investigator(Kenkyū-buntansha) 内田 研造  福井大学, 医学部, 教授 (60273009)
中嶋 秀明  福井大学, 学術研究院医学系部門, 講師 (10397276)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
KeywordsOPLL / インディアンヘッジホッグ / 内軟骨骨化 / マイクロアレイ / 免疫組織化学的染色 / 後縦靭帯骨化症 / Indian hedgehog / ectopic ossification / Sox9 / immunohistochemistry
Outline of Final Research Achievements

The genes related to chondrogenesis, including Ihh, Sox9, and Runx2, were upregulated after a 24-hour strain exposure. Furthemore, the protein expression levels of Gli2 and Gli3 were upregulated after a 6-hour cyclic tensile strain exposure, the protein expression levels of Ihh, Sox9, and Runx2 were upregulated after a 12-hour strain exposure. In addition, the Ihh, Sox9, Gli2, and Gli3 proteins were expressed in the proliferating chondrocyte layer, Runx2 was expressed in the hypertrophic chondrocyte layer, and PTHrP was expressed in both layers at the ossification front of OPLL. Our results suggest that cyclic tensile strain might enhance the expressions of some genes and proteins related to differentiation or maturation of chondrocytes, and Ihh and its related signaling might play a key role in human OPLL.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (7 results)

All 2016 2015 2014

All Presentation (7 results)

  • [Presentation] ヒト頸椎OPLL骨化巣におけるmechanical strainとIhh signalingの発現に関する検討2016

    • Author(s)
      杉田大輔、他
    • Organizer
      第31回日本整形外科基礎学術集会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2016-10-13
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヒト頸椎OPLLの骨化巣における軟骨細胞分化、肥大に関与する遺伝子およびタンパク発現に関する検討2016

    • Author(s)
      杉田大輔、他
    • Organizer
      第89回日本整形外科学会総会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-05-12
    • Related Report
      2016 Annual Research Report
  • [Presentation] OPLL骨化前線におけるIhhとその受容体のタンパク発現量と発現の局在に関する検討2016

    • Author(s)
      杉田大輔、他
    • Organizer
      第45回脊椎脊髄病学会
    • Place of Presentation
      幕張メッセ(千葉県千葉市)
    • Year and Date
      2016-04-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] OPLL骨化前線におけるIhhとその受容体GLI2、GLI3、Patched1の発現に関する検討2015

    • Author(s)
      杉田大輔, 中嶋秀明, 竹浦直人, 吉田藍, 本定和也, 内田研造
    • Organizer
      第30回日本整形外科基礎学術集会
    • Place of Presentation
      富山国際会議場
    • Year and Date
      2015-10-22
    • Related Report
      2015 Research-status Report
  • [Presentation] ヒト頸椎OPLLにおけるメカニカルストレスが骨化前線部の内軟骨骨化に関与する転写因子に与える影響の検討2015

    • Author(s)
      杉田大輔, 内田研造, 中嶋秀明, 小久保安朗, 山岸淳嗣, 吉田藍, 馬場久敏
    • Organizer
      第44回日本脊椎脊髄病学会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2015-04-16
    • Related Report
      2015 Research-status Report
  • [Presentation] メカニカルストレスはヒトOPLLにおいてIhhとその受容体の発現を増加させ軟骨細胞肥大を進行させる2014

    • Author(s)
      杉田大輔, 内田研造, 中嶋秀明, 本定和也, 坂本拓己, 山岸淳嗣, 馬場久敏
    • Organizer
      第29回日本整形外科学会基礎学術集会
    • Place of Presentation
      鹿児島(城山観光ホテル)
    • Year and Date
      2014-10-09 – 2014-10-10
    • Related Report
      2014 Research-status Report
  • [Presentation] 頚椎後縦靭帯骨化症の骨化巣における軟骨細胞分化・肥大に関する遺伝子学的解析2014

    • Author(s)
      杉田大輔, 内田研造, 中嶋秀明, 渡邉修司, 竹浦直人, 吉田幸男, 馬場久敏
    • Organizer
      第43回日本脊椎脊髄病学会学術集会
    • Place of Presentation
      京都(国立京都国際会館)
    • Year and Date
      2014-04-17 – 2014-04-19
    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi