The evaluation of protein expression and cyclic tensile strain of human OPLL
Project/Area Number |
26462235
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Orthopaedic surgery
|
Research Institution | University of Fukui |
Principal Investigator |
Sugita Daisuke 福井大学, 学術研究院医学系部門, 助教 (90596678)
|
Co-Investigator(Kenkyū-buntansha) |
内田 研造 福井大学, 医学部, 教授 (60273009)
中嶋 秀明 福井大学, 学術研究院医学系部門, 講師 (10397276)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
|
Keywords | OPLL / インディアンヘッジホッグ / 内軟骨骨化 / マイクロアレイ / 免疫組織化学的染色 / 後縦靭帯骨化症 / Indian hedgehog / ectopic ossification / Sox9 / immunohistochemistry |
Outline of Final Research Achievements |
The genes related to chondrogenesis, including Ihh, Sox9, and Runx2, were upregulated after a 24-hour strain exposure. Furthemore, the protein expression levels of Gli2 and Gli3 were upregulated after a 6-hour cyclic tensile strain exposure, the protein expression levels of Ihh, Sox9, and Runx2 were upregulated after a 12-hour strain exposure. In addition, the Ihh, Sox9, Gli2, and Gli3 proteins were expressed in the proliferating chondrocyte layer, Runx2 was expressed in the hypertrophic chondrocyte layer, and PTHrP was expressed in both layers at the ossification front of OPLL. Our results suggest that cyclic tensile strain might enhance the expressions of some genes and proteins related to differentiation or maturation of chondrocytes, and Ihh and its related signaling might play a key role in human OPLL.
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Report
(4 results)
Research Products
(7 results)