| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
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| Outline of Final Research Achievements |
The genes related to chondrogenesis, including Ihh, Sox9, and Runx2, were upregulated after a 24-hour strain exposure. Furthemore, the protein expression levels of Gli2 and Gli3 were upregulated after a 6-hour cyclic tensile strain exposure, the protein expression levels of Ihh, Sox9, and Runx2 were upregulated after a 12-hour strain exposure. In addition, the Ihh, Sox9, Gli2, and Gli3 proteins were expressed in the proliferating chondrocyte layer, Runx2 was expressed in the hypertrophic chondrocyte layer, and PTHrP was expressed in both layers at the ossification front of OPLL. Our results suggest that cyclic tensile strain might enhance the expressions of some genes and proteins related to differentiation or maturation of chondrocytes, and Ihh and its related signaling might play a key role in human OPLL.
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