Analysis of the role and impact of glia limitans in spinal cord injury

• Project/Area Number: 26462243
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Okayama University
• Principal Investigator: Yonezawa Tomoko 岡山大学, 医歯薬学総合研究科, 助教 (30304299)
• Co-Investigator(Kenkyū-buntansha): 斉藤 健司 新見公立短期大学, その他, 教授 (70270014)
• Co-Investigator(Renkei-kenkyūsha): OHTSUKA Aiji 岡山大学, 大学院医歯薬学総合研究科, 教授 (50168986)
• Research Collaborator: HOTTA Akira 岡山大学, 大学院医歯薬学総合研究科, 大学院生 ; TAKAGAKI Kazuya 岡山大学, 医学部保健学科 ; INOMOTO Saori 岡山大学, 医学部保健学科 ; SADO Yoshikazu 岡山大学, 医学部, 客員研究員 ; TAKIGAWA Tomoaki 岡山大学, 大学病院・整形外科学, 助教 ; Kadir Demircan Turgut Ozal University ; NINOMIYA Yoshifumi (元)岡山大学, 大学院医歯薬学総合研究科, 教授 (70126241)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 脊髄損傷 / 細胞外マトリックス / アストロサイト / コラーゲン

Outline of Final Research Achievements

After spinal cord injury, glia limitans are destroyed. And then new glia limitans surround the injured tissue. We examined the temporal change of type IV collagen expression and the association between the type IV collagen expression and the secondary injury cascade using spinal cord injury mouse model. We found that the type IV collagen expression increased in injured cord and the temporal expression pattern was different depending on the alpha(IV) chain. The result suggested that the interaction between the extracellular matrix and cell play a role in injured spinal cord.

Research Products

• [Int'l Joint Research] Max-Planck institute(ドイツ)
• [Journal Article] Type IV collagen α6 chain is a regulator of keratin 10 in keratinization of oral mucosal epithelium (2018)
  • Author(s): Komori T, Ono M, Hara ES, Ueda J, Nguyen HTT, Nguyen HT, Yonezawa T, Maeba T, Ono A, Takarada T, Momota R, Maekawa K, Kuboki T, Oohashi T
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 2612-2612
  • DOI: 10.1038/s41598-018-21000-0
  • Peer Reviewed / Open Access
• [Journal Article] COL4A6 is dispensable for autosomal recessive Alport syndrome. (2016)
  • Author(s): Murata T, Katayama K, Oohashi T, Jahnukainen T, Yonezawa T, Sado Y, Ishikawa E, Nomura S, Tryggvason K, Ito M.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 29450-29450
  • DOI: 10.1038/srep29450
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Early Induction of ADAMTS 1, -4, -5 and -9 in IL-Stimulated Mouse Astrocytes. (2014)
  • Author(s): Abali O, Gokce EC, Cemil B, Erdogan B, Yonezawa T, Demircan K.
  • Journal Title: Turkish Neurosurgery Volume: 24 Pages: 519-524
  • Peer Reviewed
• [Journal Article] ADAMTS4 and ADAMTS5 Knockout Mice Are Protected from Versican but Not Aggrecan or Brevican Proteolysis during Spinal Cord Injury. (2014)
  • Author(s): Demircan K, Topcu V, Takigawa T, Akyol S, Yonezawa T, Ozturk G, Ugurcu V, Hasgul R, Yigitoglu MR, Akyol O, McCulloch DR, Hirohata S.
  • Journal Title: Biomed Res Int. Volume: 2014 Pages: 693746-693746
  • DOI: 10.1155/2014/693746
  • Peer Reviewed / Open Access
• [Presentation] Functional analysis of type IV collagen alpha6 chain using spinal cord injury mouse model (2014)
  • Author(s): Yonezawa Tomoko, Akira Hotta, Saori Inomoto, Kazuya Takagaki, Yoshikazu Sado, Yoshifumi Ninomiya
  • Organizer: 結合組織学会、マトリックス研究会合同学術集会
  • Place of Presentation: ウインク愛知（愛知県名古屋市）
  • Year and Date: 2014-06-05 – 2014-06-07
• [Remarks] 岡山大学大学院 医歯薬学総合研究科 分子医化学
  • URL: http://www.okayama-u-mbb.jp/
• [Remarks] 岡山大学大学院医歯薬学総合研究科分子医化学分野
  • URL: http://www.okayama-u-mbb.jp/
• [Remarks] 岡山大学大学院医歯薬学総合研究科分子医化学分野 ホームページ
  • URL: http://www.okayama-u-mbb.jp/