Regulation of bone-associated cells differentiation and function by micro configuration of material surface

• Project/Area Number: 26462263
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Osaka University
• Principal Investigator: Myoui Akira 大阪大学, 医学部附属病院, 准教授 (10263261)
• Co-Investigator(Kenkyū-buntansha): 岡本 美奈 大阪大学, 医学部附属病院, 助教 (50457008) ; 吉川 秀樹 大阪大学, 医学系研究科, 理事・副学長 (60191558)
• Co-Investigator(Renkei-kenkyūsha): HAMAGUCHI Satoshi 大阪大学, 工学部, 教授 (60301826)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 骨分化 / バイオマテリアル / 微細加工技術 / 骨芽細胞 / 破骨細胞 / 微細表面構造

Outline of Final Research Achievements

Microfabrication technique using plasma etching make it possible to pattern pillar shape on the quartz and cocave/convex micro configuration on the polystyrene materials. It was revealed that the ability of a cell to proliferate was down regulated and bone formation was promoted on the microfabricated culture materials in the study using bone metabolism-associated cells like osteoblast and osteoclast. The number of osteoclast formation was altered according to the difference of scaffold materials. So it was indicated the possibility of controling the capability of bone metabolism associated cells by fabricating the adequate shape of cell attachment surface and using adequate materials.

Research Products

• [Journal Article] Preparation of Scaffold-Free Tissue-Engineered Constructs Derived from Human Synovial Mesenchymal Stem Cells Under Low Oxygen Tension Enhances Their Chondrogenic Differentiation Capacity (2016)
  • Author(s): Yasui, Y., Chijimatsu, R., Hart, D.A., Koizumi, K., Sugita, N., Shimomura, K., Myoui, A.., Yoshikawa, H., Nakamura, N.
  • Journal Title: Tissue Engineering Part A Volume: 22 Issue: 5-6 Pages: 490-500
  • DOI: 10.1089/ten.tea.2015.0458
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 運動器疾患の治療におけるバイオマテリアルの位置づけと今後のニーズ. (2016)
  • Author(s): 名井陽
  • Organizer: 日本バイオマテリアル学会シンポジウム 2016
  • Place of Presentation: 福岡市
  • Year and Date: 2016-11-21
• [Presentation] Plasma-based Functionalization of Polystyrene Surfaces of Cell Culture Plates (2016)
  • Author(s): 1.Nishiyama K, Ito T, Sugimoto S, Gotoh K, Isobe M, Okamoto M, Myoui A, Yoshikawa H, and Hamaguchi S.
  • Organizer: the AVS 63rd International Symposium & Exhibition
  • Place of Presentation: Nashville, TN, USA
  • Year and Date: 2016-11-06
  • Int'l Joint Research