Antitumor effects of transcutaneous carbon dioxide therapy on stemness in bone and soft tissue sarcomas
Project/Area Number |
26462265
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Orthopaedic surgery
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Research Institution | Kobe University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
原 仁美 神戸大学, 医学部附属病院, 助教 (40437489)
大西 康央 神戸大学, 医学部附属病院, 医員 (50707122)
秋末 敏宏 神戸大学, 保健学研究科, 教授 (90379363)
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Research Collaborator |
FUKASE Naomasa
MINODA Masaya
MORISHITA Masayuki
HARADA Risa
TAKEMORI Toshiyuki
KAMATA Etsuko
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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Keywords | 骨軟部肉腫 / 未分化能 / 幹細胞 / ミトコンドリア機能 / 炭酸ガス経皮投与 / 腫瘍内低酸素環境 / 炭酸ガス経皮治療 / 肉腫幹細胞 / 低酸素環境 |
Outline of Final Research Achievements |
To establish a novel therapeutic strategy for bone and soft tissue sarcomas, we hypothesized that "Pluripotency" and "Mitochondrial dysfunction" would be the main cause of sarcoma progression, and evaluated antitumor effects of hypoxia improvements by the transcutaneous carbon dioxide (CO2) therapy on sarcomas. We revealed that iPS-related gene expression and mitochondrial dysfunction were associated with sarcoma progression, and that both of them were activated by hypoxic condition. In addition, transcutaneous CO2 therapy showed strong antitumor effects on sarcoma cells by decreased iPS-related gene expression and increased mitochondrial activity. The findings in this study strongly suggest that "Pluripotency" and "Mitochondrial dysfunction" should be the key of sarcoma progression, and that transcutaneous CO2 therapy can be an innovative therapeutic tool for sarcomas.
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Report
(4 results)
Research Products
(27 results)
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[Journal Article] AICAR induces mitochondrial apoptosis in human osteosarcoma cells through an AMPK-dependent pathway.2017
Author(s)
Morishita M, Kawamoto T, Hara H, Onishi Y, Ueha T, Minoda M, Katayama E Takemori T, Fukase N, Kurosaka M, Kuroda R, Akisue T
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Journal Title
Int J Oncol
Volume: 50
Issue: 1
Pages: 23-30
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Antitumor effect of YM155, a novel small-molecule survivin suppressant, via mitochondrial apoptosis in human MFH/UPS.2015
Author(s)
Minoda M, Kawamoto T, Ueha T, Kamata E, Morishita M, Harada R, Toda M, Onishi Y, Hara H, Kurosaka M, Akisue T.
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Journal Title
International Journal of Oncology
Volume: 47(3)
Issue: 3
Pages: 891-899
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] AICAR induces Apoptosis in Human Osteosarcoma Cells through AMPK-dependent PGC-1α/TFAM/Mitochondrial Pathway2016
Author(s)
Masayuki Morishita, Teruya Kawamoto, Takeshi Ueha, Hitomi Hara, Naomasa Fukase ,Masaya Minoda, Etsuko Kamata, Toshiyuki Takemori, Kuroda Ryosuke, Toshihiro Akisue, Masahiro Kurosaka
Organizer
ORS 2016 Annual Meeting
Place of Presentation
アメリカ合衆国(オーランド)
Year and Date
2016-03-05
Related Report
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[Presentation] Apoptotic Effect of AICAR on Osteosarcoma Cell Lines2015
Author(s)
Masayuki Morishita, Teruya Kawamoto, Toshihiro Akisue, Hitomi Hara, Naomasa Fukase, Masaya Minoda, Etsuko Kamata, Toshiyuki Takemori, Takeshi Ueha, Masahiro Kurosaka
Organizer
2015 Connective Tissue Oncology Society
Place of Presentation
アメリカ合衆国(ソルトレークシティー)
Year and Date
2015-11-04
Related Report
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[Presentation] 炭酸ガス経皮投与による低酸素環境の改善は悪性線維性組織球腫のiPS関連遺伝子の発現を低下させる2014
Author(s)
原田 理沙, 河本 旭哉, 上羽 岳志, 森下 雅之, 蓑田 正也, 戸田 光紀, 大西 康央, 原 仁美, 酒井 良忠, 黒坂 昌弘, 秋末 敏宏
Organizer
第29回日本整形外科学会基礎学術集会
Place of Presentation
鹿児島,日本
Year and Date
2014-10-09 – 2014-10-10
Related Report
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[Presentation] 炭酸ガス経皮投与は骨・軟部肉腫におけるiPS関連遺伝子の発現を低下させる2014
Author(s)
原田 理沙, 河本 旭哉, 上羽 岳志, 森下 雅之, 蓑田 正也, 戸田 光紀, 大西 康央, 原 仁美, 酒井 良忠, 黒坂 昌弘, 秋末 敏宏
Organizer
第87回日本整形外科学会学術総会
Place of Presentation
神戸,日本
Year and Date
2014-05-22 – 2014-05-25
Related Report
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