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Regeneration using adipose-derived stem cells and fluorescent imaging evaluation for a defect after bone and soft tissue tumor surgery

Research Project

Project/Area Number 26462271
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Orthopaedic surgery
Research InstitutionKanazawa University

Principal Investigator

Hayashi Katsuhiro  金沢大学, 附属病院, 助教 (80507054)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords脂肪由来幹細胞 / 骨軟部腫瘍
Outline of Final Research Achievements

We compared osteogenic and adipogenic differentiation potency between MSCs isolated and cultured from normal adipose tissues and atypical lipomatous tumor/well-differentiated liposarcoma (ALT/WDL) from the same patients. We found greater osteogenic differentiation potency in ALT/WDL-derived MSCs than in normal fat-derived MSCs. In adipogenic differentiation, contrary to our expectations, more fatty acid droplets were observed in normal fatderived MSCs than in ALT/WDL-derived MSCs. In osteogenic differentiation, as expected, MSCs derived from ALT/WDL had higher differentiation potency than normal fat-derived MSCs. The gap of osteogenic differentiation potency based on ALP staining between ALT/WDL-derived MSCs and normal fat-derived MSCs disappeared as Nutlin-3 concentration increased, suggesting that greater osteogenic differentiation could be caused by amplied MDM2 in ALT/WDL.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report

Research Products

(16 results)

All 2017 2016 2015 2014

All Journal Article (7 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results,  Open Access: 4 results,  Acknowledgement Compliant: 2 results) Presentation (9 results) (of which Int'l Joint Research: 4 results)

  • [Journal Article] Do Mesenchymal Stem Cells Derived From Atypical Lipomatous Tumors Have Greater Differentiation Potency Than Cells From Normal Adipose Tissues?2017

    • Author(s)
      Inatani H, Yamamoto N, Hayashi K, Kimura H, Takeuchi A, Miwa S, Higuchi T, Abe K, Taniguchi Y, Yamada S, Asai K, Otsuka T, Tsuchiya H.
    • Journal Title

      J Bone Oncol

      Volume: 印刷中 Pages: 1693-1701

    • DOI

      10.1007/s11999-017-5259-z

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] 運動器再生医療における脂肪由来幹細胞の潜在能力2016

    • Author(s)
      林克洋
    • Journal Title

      臨床整形外科

      Volume: 51 Pages: 635-639

    • NAID

      40020890789

    • Related Report
      2016 Annual Research Report
  • [Journal Article] The paracrine effect of adipose-derived stem cells inhibits osteoarthritis progression.2015

    • Author(s)
      Kuroda K, Kabata T, Hayashi K, Maeda T, Kajino Y, Iwai S, Fujita K, Hasegawa K, Inoue D, Sugimoto N, Tsuchiya H.
    • Journal Title

      BMC Musculoskelet Disord

      Volume: 16

    • DOI

      10.1186/s12891-015-0701-4

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Salvage method for unplanned excision of soft tissue sarcoma: long-term results of second-look surgery following radio-hyperthermo-chemotherapy.2015

    • Author(s)
      Hayashi K, Yamada S, Inatani H, Okamoto H, Takeuchi A, Nishida H, Yamamoto N, Tsuchiya H, Otsuka T
    • Journal Title

      Anticancer Res.

      Volume: 35 Pages: 493-498

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Factors that influence functional outcome after total or subtotal scapulectomy: Japanese Musculoskeletal Oncology Group (JMOG) study.2014

    • Author(s)
      Hayashi K, Iwata S, Ogose A, Kawai A, Ueda T, Otsuka T, Tsuchiya H.
    • Journal Title

      PLoS One

      Volume: 9

    • DOI

      10.1371/journal.pone.0100119

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Uncultured autogenous adipose-derived regenerative cells promote bone formation during distraction osteogenesis in rats.2014

    • Author(s)
      Nomura I, Watanabe K, Matsubara H, Hayashi K, Sugimoto N, Tsuchiya H.
    • Journal Title

      Clin Orthop Relat Res

      Volume: 472 Pages: 3798-806

    • DOI

      10.1007/s11999-014-3608-8

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] A novel method to apply osteogenic potential of adipose derived stem cells in orthopaedic surgery2014

    • Author(s)
      Fang X, Murakami H, Demura S, Hayashi K, Matsubara H, Kato S, Yoshioka K, Inoue K, Ota T, Shinmura K, Tsuchiya H
    • Journal Title

      PLoS One

      Volume: 9(2)

    • DOI

      10.1371/journal.pone.0088874

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] From Fat to Bone: Making Adipose Derived Stem Cell Sheets and Osteogenesis of Adipose Derived Stem Cell Sheets2017

    • Author(s)
      Xiang Fang, Katsuhiro Hayash, Hideki Murakami, Hiroyuki Tsuchiya
    • Organizer
      American Academy of Orthopedic Surgeons
    • Place of Presentation
      Convention Center (USA)
    • Year and Date
      2017-03-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 脂肪腫と異型脂肪腫瘍腫瘍/高分化型脂肪肉腫70例の臨床的特徴の比較2016

    • Author(s)
      稲谷弘幸, 山本憲男, 林克洋, 武内章彦, 三輪真嗣, 樋口貴史, 阿部健作, 谷口裕太, 土屋弘行
    • Organizer
      第49回日本整形外科学会骨軟部学術集会
    • Place of Presentation
      東京ドームホテル(東京)
    • Year and Date
      2016-07-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] The Comparative Analysis Of Differentiation Potency Between The Mesenchymal Stem Cells Derived From The Same Human Normal Adipose Tissue And Atypical Lipomatous Tumor2016

    • Author(s)
      Inatani H, Yamamoto N, Hayashi K, Kimura H, Takeuchi A, Miwa S, Otsuka T, Tsuchiya H
    • Organizer
      11th Asia Pacific Musculoskeletal Tumor Society Meeting
    • Place of Presentation
      Grand Copthorne Waterfront (シンガポール)
    • Year and Date
      2016-04-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A novel method to apply adipose-derived stem cells to bone and soft tissue defects after tumor excision.2015

    • Author(s)
      Hayashi K
    • Organizer
      International Society of Limb Salvage & Musculoskeletal Tumor Society combined meeting
    • Place of Presentation
      W Marriott Orlando Grande Lakes (USA)
    • Year and Date
      2015-10-06
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] The comparative analysis of differentiation potency between the mesenchymal stem cells derived from the same human normal adipose tissue and atypical lipomatous tumor2015

    • Author(s)
      Inatani H, Yamamoto N, Hayashi K
    • Organizer
      International Society of Limb Salvage & Musculoskeletal Tumor Society combined meeting
    • Place of Presentation
      W Marriott Orlando Grande Lakes (USA)
    • Year and Date
      2015-10-06
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 骨端を含む悪性骨腫瘍に対して液体窒素処理した自家骨軟骨移植術の経験2015

    • Author(s)
      林克洋
    • Organizer
      第125回中部日本整形外科災害外科学会・学術集会
    • Place of Presentation
      ウインクあいち (名古屋)
    • Year and Date
      2015-10-02
    • Related Report
      2015 Research-status Report
  • [Presentation] Case analysis of poor prognostic bone sarcoma patient who underwent tumor-bearing frozen autograft2014

    • Author(s)
      林克洋
    • Organizer
      低温医学学会総会
    • Place of Presentation
      野依記念学術交流館(愛知県名古屋市)
    • Year and Date
      2014-11-13 – 2014-11-14
    • Related Report
      2014 Research-status Report
  • [Presentation] The diagnosis using MDM2 and CDK4 for low-grade osteosarcoma and treatment with biologic reconstruction2014

    • Author(s)
      Katsuhiro Hayashi
    • Organizer
      Societe Internationale de Chirurgie Orthopedique et de Traumatologie
    • Place of Presentation
      SulAmerica Convention Centre (Brazil)
    • Year and Date
      2014-11-09 – 2014-11-22
    • Related Report
      2014 Research-status Report
  • [Presentation] マウス内で肉腫細胞の転移臓器播種リアルタイムイメージング2014

    • Author(s)
      林克洋
    • Organizer
      第 23回日本がん転移学会
    • Place of Presentation
      文化ホール(石川県金沢市)
    • Year and Date
      2014-07-10 – 2014-07-11
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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