Development of bone healing by direct converted osteoblast like cells

Research Project

Project/Area Number	26462272
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Orthopaedic surgery
Research Institution	Kyoto Prefectural University of Medicine
Principal Investigator	Taniguchi Daigo 京都府立医科大学, 医学(系)研究科(研究院), 客員講師 (00642092)
Co-Investigator(Kenkyū-buntansha)	白井寿治京都府立医科大学, 医学(系)研究科(研究院), 准教授 (20397186) 小田良京都府立医科大学, 医学(系)研究科(研究院), 講師 (80516469) 藤原浩芳京都府立医科大学, 医学(系)研究科(研究院), 准教授 (90381962)
Project Period (FY)	2014-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000) Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords	再生医療 / 骨芽細胞 / ダイレクト・リプログラミング / 再生医学 / 遺伝子導入 / ダイレクトリプログラミング
Outline of Final Research Achievements	Early bone fusion after fracture reduction and bone graft surgery is important to minimize disuse syndrome. Direct reprogramming is capable of producing many target cells in a short period of time from autogenous tissue at low cost without going through stem cells. And we have developed osteoblast like cells from fibroblasts using this technique and their administration methods were studied. The osteoblast-like cells were expressed at the gene and protein level, and bone matrix was produced. Also, even if chemokine receptor gene was introduced to improve the survival rate, there was no significant decrease in induction efficiency.