A combined therapy with PRP and hyaluronan for tendinopathy

Research Project

Project/Area Number	26462278
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Orthopaedic surgery
Research Institution	Jikei University School of Medicine
Principal Investigator	Yoshida Mamoru 東京慈恵会医科大学, 医学部, 准教授 (10266702)
Co-Investigator(Kenkyū-buntansha)	舟崎裕記東京慈恵会医科大学, 医学部, 准教授 (70199412) 丸毛啓史東京慈恵会医科大学, 医学部, 教授 (70199925)
Project Period (FY)	2014-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000) Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords	PRP / tendinopathy / ＰＲＰ / ｔｅｎｄｉｎｏｐａｔｈｙ / ａｐｏｔｏｓｉｓ / platelet-rich plasma / hyaluronan / 腱付着部症 / トレッドミル / 動物モデル
Outline of Final Research Achievements	The present study demonstrated that an injection of PRP at the platelet concentration of a half million to a million/micro L against the animal tendinopathy inhibited the expressions of pain-inducing molecule resulted in the loss of pain and the increase of the spontaneous activity and a PRP therapy accelerated the reparative reaction at the level for the gene-expression or histopathology. Moreover, a PRP therapy inhibited the occurrence of cellular apoptosis and improved the acidic condition. These findings in the study suggested that a PRP therapy was effective and safety for the treatment of the tendinopathy in animal model. An injection of hyaluronan was also effective for the tendinopathy therapy, however, the efficacy was weaker than that of a PRP therapy. No synergistic effect combined with a hyaluronan and a PRP was observed in the animal model study. Further investigation to determine the optimal concentration of the platelets in PRP is necessary.