Analysis of circulating tumor cells using telomerase-specific adenovirus in patients with sarcoma and metastatic breast cancer

• Project/Area Number: 26462280
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Aichi Medical University (2015-2016); Department of Clinical Research, National Hospital Organization Kure Medical Center (2014)
• Principal Investigator: Matsuo Toshihiro 愛知医科大学, 医学部, 講師 (90397977)
• Co-Investigator(Kenkyū-buntansha): 杉田 孝 独立行政法人国立病院機構(呉医療センター臨床研究部), その他部局等, その他 (40235883) ; 谷山 清己 独立行政法人国立病院機構(呉医療センター臨床研究部), その他部局等, その他 (50163639)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 末梢血循環癌細胞 / 肉腫 / 乳癌 / 肺転移 / 骨転移 / テロメラーゼ / 末梢循環癌細胞 / 血中循環がん細胞 / 軟部肉腫 / ウイルス / 血中循環癌細胞

Outline of Final Research Achievements

We attempted to detect viable circulating tumor cells (CTCs) in peripheral blood from sarcoma and bony metastatic breast cancer patients using a telomerase-specific viral agent. The number of CTCs postoperatively was significantly higher in sarcoma patients with metastasis than in those without metastasis (p = 0.022). The number of postoperative CTCs was significantly higher in dead sarcoma patients than in living patients (p = 0.014). A significant relationship was found between the number of CTCs and both occurrence of lung metastasis and prognosis in patients with sarcoma. Detection of CTCs using this method may be useful for prognostic evaluation of sarcoma patients. CTCs were detected in one patient who showed resistance for chemotherapy and hormone therapy in bony metastatic breast cancer patient. The number of CTCs may correlate with sensitivity to chemotherapy or hormone therapy in metastatic breast cancer patients.

Research Products

• [Journal Article] Circulating tumor cells using hTERT specific replication selective adenovirus in patients with soft tissue sarcoma (2017)
  • Author(s): Toshihiro Matsuo, et al.
  • Journal Title: International Journal of Clinical and Experimental Medicine (in press) Volume: 印刷中
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Circulating tumor cells using hTERT-specific replication-selective adenovirus as a prognostic factor in soft tissue sarcoma. (2016)
  • Author(s): Toshihiro Matsuo, et al.
  • Organizer: Connective Tissue Oncology Society 2016 Annual Meeting
  • Place of Presentation: ポルトガル リスボン
  • Year and Date: 2016-11-09
  • Int'l Joint Research
• [Presentation] Current concept of management in aneurysmal bone cyst. (2016)
  • Author(s): Matsuo T
  • Organizer: Grandround Musculoskeletal Tumor
  • Place of Presentation: バリ インドネシア
  • Year and Date: 2016-03-11
  • Invited
• [Presentation] Detecting circulating tumor cells by hTERT-specific replication-selective adenovirus in postoperative sarcoma patients (2015)
  • Author(s): Matsuo T, Sugita T, Shimose S, Hamada N, Deie M, Urata Y
  • Organizer: ESMO-Asia 2015
  • Place of Presentation: シンガポール
  • Year and Date: 2015-12-18
  • Int'l Joint Research
• [Presentation] Evaluation of human circulating tumor cells by hTERT- specific replication- selective adenovirus in soft tissue sarcoma (2014)
  • Author(s): Matsuo T, Hamada N, Hachisuka H, Hamasaki T, Izuta Y, Mori R, Ohkawa S, Harada T, Urata Y, Sugita T
  • Organizer: 39th European Society for Medical Oncology 2014 Congress
  • Place of Presentation: Madrid, Sｐain
  • Year and Date: 2014-09-26 – 2014-09-30
• [Presentation] hTERT依存性アデノウイルス製剤を用いた軟部肉腫における末梢循環癌細胞の検討 (2014)
  • Author(s): 松尾俊宏，杉田孝，濱田宜和，蜂須賀裕己，濱崎貴彦，泉田泰典，仁井谷学，大川新吾，原田崇弘，片桐伸悦，浦田泰生，谷山清己
  • Organizer: 第47回日本整形外科学会・骨軟部腫瘍学術集会
  • Place of Presentation: 大阪市
  • Year and Date: 2014-07-17 – 2014-07-18
• [Remarks] 呉医療センター中国がんセンターホームページ
  • URL: http://gw1.kure-nh.go.jp/department/orthopaedic/03.html