| Project/Area Number |
26462287
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
Jinno Tetsuya 東京医科歯科大学, 大学院医歯学総合研究科, 准教授 (90343152)
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| Co-Investigator(Kenkyū-buntansha) |
辻 邦和 東京医科歯科大学, 大学院医歯学総合研究科, 寄附講座准教授 (20323694)
宗田 大 東京医科歯科大学, 大学院医歯学総合研究科, 非常勤講師 (50190864)
関矢 一郎 東京医科歯科大学, 再生医療研究センター, 教授 (10345291)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 変形性膝関節症 / 動物モデル / 卵巣摘除 / トレッドミル / 滑膜炎症 / 関節軟骨 / 力学的負荷 / 滑膜炎 / Osteoarthritis / ovariectomy / forced running / synovitis / genetic background / 変性性関節症 / メカニカルストレス / 遺伝的背景 |
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| Outline of Final Research Achievements |
Although osteoarthritis (OA) is a multifactorial disease, little is reported regarding the cooperative interaction between these factors on cartilage metabolism. Here we examined the synergistic effect of ovariectomy (OVX) and excessive mechanical stress (forced running) on articular cartilage homeostasis in a mouse model resembling a human post menopause condition. Histological analysis revealed that OVX or forced running respectively had subtle effect on cartilage degeneration. However, the combination of OVX and forced running synergistically enhanced synovitis and articular cartilage degeneration. Interestingly, these results were observed specifically in Balb/c mice, since we did not observe any synergistic effects of OVX and forced running on articular cartilage degeneration in C57Bl/6J mice.
Since surgical procedure was not performed on the knee joint directly in this model, this model is useful in addressing the molecular pathogenesis of naturally occurring OA.
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