| Project/Area Number |
26462288
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | University of Fukui |
|---|
Principal Investigator |
Miyazaki Tsuyoshi 福井大学, 学術研究院医学系部門(附属病院部), 講師 (80324169)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
内田 研造 福井大学, 医学部, 教授 (60273009)
坂本 拓己 福井大学, 医学部附属病院, 医員 (40634837)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
|
|---|
| Keywords | 変形性膝関節症 / 軟骨再生 / MIAモデル / ヒアルロン酸 / CD271 / BMSc / 脂肪由来幹細胞 / guineapig / OA自然発症動物モデル |
|---|
| Outline of Final Research Achievements |
At 5 weeks post transplantation, partial cartilage repair was noted in the HA-MSC group but not in the other groups. Examination of CFDA-SE-labeled cells demonstrated migration, differentiation, and proliferation of MSC in the HA-MSC group. There was strong immunostaining for type II collagen around both residual chondrocytes and transplanted MSCs in the OA cartilage.
This scaffold-free and technically undemanding technique appears to result in the regeneration of articular cartilage in the spontaneous OA animal model. Although further examination of the long-term effects of transplantation is necessary, the findings suggest that intra-articular injection of HA-MSC mixture is potentially beneficial for OA.
|
