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Roles of AMP-activated protein kinase in bone metabolism

Research Project

Project/Area Number 26462289
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Orthopaedic surgery
Research InstitutionGifu University

Principal Investigator

TOKUDA HARUHIKO  岐阜大学, 大学院医学系研究科, 非常勤講師 (10397325)

Co-Investigator(Kenkyū-buntansha) 小澤 修  岐阜大学, 大学院医学系研究科, 教授 (90225417)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords骨代謝 / エネルギー代謝 / 骨芽細胞 / AMPキナーゼ / プロスタグランジン / オステオプロテジェリン / MAPキナーゼ / プロスタグランジンD2 / オステオプレテジェリン / p44/p42 MAPキナーゼ / SAPK/JNK / prostaglandin E2
Outline of Final Research Achievements

AMP-activated protein kinase (AMPK), a key enzyme sensing cellular energy metabolism, is currently known to regulate multiple metabolic pathways. Osteoprotegerin plays a pivotal role in the regulation of bone metabolism by inhibiting osteoclast activation. We have previously reported that the activation of the mitogen-activated protein (MAP) kinase superfamily including p38 MAP kinase, p44/p42 MAP kinase and stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) are involved in the synthesis of osteoprotegerin stimulated by prostaglandin D2 (PGD2), PGE1 or PGE2 in osteoblast-like MC3T3-E1 cells. On the basis of these findings, we herein investigated the implication of AMPK in the osteoprotegerin synthesis induced by PGD2, PGE1 or PGE2 in these cells. Our results strongly suggest that AMPK acts as a positive regulator in the osteoprotegerin synthesis stimulated by PGD2, PGE1 or PGE2 in osteoblasts.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (6 results)

All 2016 2015 2014 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Remarks (2 results)

  • [Journal Article] Possible involvement of AMP-activated protein kinase in PGE1-induced synthesis of osteoprotegerin in osteoblasts.2016

    • Author(s)
      Kainuma S, Otsuka T, Kuroyanagi G, Yamamoto N, Matsushima-Nishiwaki R, Kozawa O and Tokuda H.
    • Journal Title

      Exp Ther Med.

      Volume: 5 Pages: 2042-2048

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Regulation by AMP-activated protein kinase of PGE2-induced osteoprotegerin synthesis in osteoblasts.2016

    • Author(s)
      Kainuma S, Otsuka T, Kuroyanagi G, Yamamoto N, Matsushima-Nishiwaki R, Kozawa O and Tokuda H.
    • Journal Title

      Mol Med Rep.

      Volume: 4 Issue: 4 Pages: 3363-3369

    • DOI

      10.3892/mmr.2016.4900

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] PGD2 stimulates osteoprotegerin synthesis via AMP-activated protein kinase in osteoblasts: regulation of ERK and SAPK/JNK.2015

    • Author(s)
      Kainuma S, Tokuda H, Kuroyanagi G, Yamamoto N, Ohguchi R, Fujita K, Matsushima-Nishiwaki R, Kozawa O and Otsuka T.
    • Journal Title

      Prostaglandins Leukot. Essent. Fatty Acids.

      Volume: 101 Pages: 23-29

    • DOI

      10.1016/j.plefa.2015.08.003

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Resveratrol suppresses prostaglandin F(2α)-induced osteoprotegerin synthesis in osteoblasts: inhibition of the MAP kinase signaling.2014

    • Author(s)
      Kuroyanagi G, Tokuda H, Matsushima-Nishiwaki R, Kondo A, Mizutani J, Kozawa O, Otsuka T.
    • Journal Title

      Arch Biochem Biophys.

      Volume: 542 Pages: 39-45

    • DOI

      10.1016/j.abb.2013.12.002

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Remarks]

    • URL

      http://www.med.gifu-u.ac.jp/pharma/index.htm

    • Related Report
      2016 Annual Research Report
  • [Remarks] 岐阜大学大学院医学系研究科 分子・構造学講座 薬理病態学分野

    • URL

      http://www.med.gifu-u.ac.jp/pharma/index.htm

    • Related Report
      2015 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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