| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
The chondrocytes cultured at high cell densities in the 2.0 × 107cells/cm2 group only became concentrated and formed plate-like construct similar to native articular cartilage by macroscopic and histological assessments. Statistical analysis on the matrix gene expression demonstrated that the levels of type II collagen and aggrecan mRNA of the 2.0 × 107cells/cm2 group were significantly higher than the other 2 cell-density groups. Interestingly, the LIPUS application only lead to a statistically significant enhancement of aggrecan gene expression in the 2.0 × 107 cells/cm2 group. The current study presents a semi-open static culture system that facilitates production of the scaffold-free constructs from the monolayer-cultured chondrocytes. It suggests that LIPUS application enhances matrix production in the construct, and its combination with the scaffold-free construct might become a feasible tool for production of implantable constructs of better quality.
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