Low-Intensity Pulsed Ultrasound Promoted Matrix Synthesis of Scaffold-free Cartilage Produced in High Density Static Semi-open Culture System
| Project/Area Number |
26462292
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
今井 晋二 滋賀医科大学, 医学部, 教授 (90283556)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 再生医学 / 軟骨細胞 / 整形外科 |
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| Outline of Final Research Achievements |
The chondrocytes cultured at high cell densities in the 2.0 × 107cells/cm2 group only became concentrated and formed plate-like construct similar to native articular cartilage by macroscopic and histological assessments. Statistical analysis on the matrix gene expression demonstrated that the levels of type II collagen and aggrecan mRNA of the 2.0 × 107cells/cm2 group were significantly higher than the other 2 cell-density groups. Interestingly, the LIPUS application only lead to a statistically significant enhancement of aggrecan gene expression in the 2.0 × 107 cells/cm2 group. The current study presents a semi-open static culture system that facilitates production of the scaffold-free constructs from the monolayer-cultured chondrocytes. It suggests that LIPUS application enhances matrix production in the construct, and its combination with the scaffold-free construct might become a feasible tool for production of implantable constructs of better quality.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Improved quality of cartilage repair by bone marrow mesenchymal stem cells for treatment of an osteochondral defect in a cynomolgus macaque model2015
Author(s)
Araki S, Imai S, Ishigaki H, Mimura T, Nishizawa K, Ueba H, Kumagai K, Kubo M, Mori K, Ogasawara K, Matsusue Y
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Journal Title
Acta Orthop
Volume: 86
Issue: 1
Pages: 119-126
DOI
Related Report
Peer Reviewed
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[Presentation] 私の膝関節外科のあゆみ2014
Author(s)
松末吉隆
Organizer
第47回中国・四国整形外科学会
Place of Presentation
尾道市
Year and Date
2014-11-08 – 2014-11-09
Related Report
Invited
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