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Functional analysis of Siglec-15 in osteoclasts.

Research Project

Project/Area Number 26462295
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Orthopaedic surgery
Research InstitutionNara Institute of Science and Technology

Principal Investigator

Ishida-Kitagawa Norihiro  奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (30294284)

Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords骨・軟骨代謝学 / 破骨細胞 / 骨吸収 / NFATc1 / ITAM / 糖鎖 / シグナル伝達 / 骨代謝 / 免疫学
Outline of Final Research Achievements

Signaling cascade through ITAM of DAP12 is essential for formation of functional osteoclasts. In this research, first we elucidated the functional amino acid sequence in cytoplasmic region of Siglec-15 and through this sequence microtubule related protein interacts with Siglec-15. From these findings, we constructed chimeric protein of Siglec-15 and DAP12 and found the expression of this artificial protein effectively rescued the phenotype of Siglec-15 KO mice in vitro. We established transgenic mice which express this chimeric protein in osteoclast specific manner and mate this mice with Siglec-15 KO mice which shows mild osteopetrosis as same as DAP12 KO mice. Finally, we could successfully obtain tibias and femurs from Siglec-15 KO background mice with or without transgenic allele.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (2 results)

All 2017 2015

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (1 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] BNIP-2 binds phosphatidylserine, localizes to vesicles, and is transported by kinesin-1.2015

    • Author(s)
      Akamatsu R, Ishida-Kitagawa N, Aoyama T, Oka C, Kawaichi M
    • Journal Title

      Genes to Cells.

      Volume: 20 Issue: 2 Pages: 135-152

    • DOI

      10.1111/gtc.12209

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Cell Proliferation and Differentiation as Target of Therapy2017

    • Author(s)
      Norihiro Ishida-Kitagawa
    • Organizer
      The 2nd Universitas Muhammadiyah Purwokerto-Pharmacy International Conference And 8th Indonesian Society for Cancer Chemoprevension Symposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited

URL: 

Published: 2014-04-04   Modified: 2019-03-29  

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