| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Signaling cascade through ITAM of DAP12 is essential for formation of functional osteoclasts. In this research, first we elucidated the functional amino acid sequence in cytoplasmic region of Siglec-15 and through this sequence microtubule related protein interacts with Siglec-15. From these findings, we constructed chimeric protein of Siglec-15 and DAP12 and found the expression of this artificial protein effectively rescued the phenotype of Siglec-15 KO mice in vitro. We established transgenic mice which express this chimeric protein in osteoclast specific manner and mate this mice with Siglec-15 KO mice which shows mild osteopetrosis as same as DAP12 KO mice. Finally, we could successfully obtain tibias and femurs from Siglec-15 KO background mice with or without transgenic allele.
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