| Project/Area Number |
26462307
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kagoshima University |
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Principal Investigator |
Ishidou Yasuhiro 鹿児島大学, 医歯学総合研究科, 特任准教授 (10300740)
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| Co-Investigator(Kenkyū-buntansha) |
前田 真吾 鹿児島大学, 医歯学総合研究科, 特任准教授 (60353463)
小宮 節郎 鹿児島大学, 医歯学域医学系, 教授 (30178371)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | TGF-β / 軟骨細胞分化 / Myct1 / BMP / 軟骨細胞 / TGF-βシグナル / c-Myc / MYCT1 / c-MYC / 軟骨肉腫 |
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| Outline of Final Research Achievements |
During endochondral ossification process, TGF-β signaling promotes chondrocyte differentiation while inhibits hypertrophic chondrocyte maturation, which underlying mechanisms are not clear. We found that TGF-β1 strongly induced expression of Myct1 gene in human chondrocyte cell line. siRNA-mediated knockdown of c-Myc diminished TGF-β1-induced Myct1 expression, suggesting that c-Myc is indispensable for the expression. However, silencing of Myct1 did not affect TGF-β1-mediated chondrocyte differentiation. Then, we next focused on PEG10 gene which was suppressed by TGF-β1. Induction of PEG10 inhibited TGF-β signaling, suggesting PEG10 to be a novel inhibitory factor for TGF-β pathway.
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