Myct1 as a contact point of TGF-beta and c-Myc; analysis of the function
Project/Area Number |
26462307
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Orthopaedic surgery
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Research Institution | Kagoshima University |
Principal Investigator |
Ishidou Yasuhiro 鹿児島大学, 医歯学総合研究科, 特任准教授 (10300740)
|
Co-Investigator(Kenkyū-buntansha) |
前田 真吾 鹿児島大学, 医歯学総合研究科, 特任准教授 (60353463)
小宮 節郎 鹿児島大学, 医歯学域医学系, 教授 (30178371)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | TGF-β / 軟骨細胞分化 / Myct1 / BMP / 軟骨細胞 / TGF-βシグナル / c-Myc / MYCT1 / c-MYC / 軟骨肉腫 |
Outline of Final Research Achievements |
During endochondral ossification process, TGF-β signaling promotes chondrocyte differentiation while inhibits hypertrophic chondrocyte maturation, which underlying mechanisms are not clear. We found that TGF-β1 strongly induced expression of Myct1 gene in human chondrocyte cell line. siRNA-mediated knockdown of c-Myc diminished TGF-β1-induced Myct1 expression, suggesting that c-Myc is indispensable for the expression. However, silencing of Myct1 did not affect TGF-β1-mediated chondrocyte differentiation. Then, we next focused on PEG10 gene which was suppressed by TGF-β1. Induction of PEG10 inhibited TGF-β signaling, suggesting PEG10 to be a novel inhibitory factor for TGF-β pathway.
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Report
(4 results)
Research Products
(10 results)
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[Presentation] TGF-βシグナルとPEG10の内軟骨腫と軟骨肉腫の診断鑑別マーカーとしての可能性と機能解析2016
Author(s)
篠原直弘, 前田真吾, 松山金寛, 八尋雄平, 今村勝之, 河村一郎, 瀬戸口啓夫, 永野聡, 横内雅博, 石堂康弘, 小宮節郎
Organizer
第34回日本骨代謝学会
Place of Presentation
大阪国際会議場(大阪府・大阪市)
Year and Date
2016-07-20
Related Report
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[Presentation] Expression of Paternally Expressed Gene 10 (PEG10) is Negatively Associated with Malignancy Grading of Human Chondrosarcoma; the Role of PEG10 to Prevent Bone Morphogenetic Protein (BMP)-p38 MAPK Signaling-mediated Induction of Matrix Metalloproteinase (MMP)-1 to Suppress the Invasive Activity2016
Author(s)
Naohiro Shinohara, Shingo Maeda, Kanehiro Matsuyama, Yuhei Yahiro, Katsuyuki Imamura, Ichiro Kawamura, Takao Setoguchi, Satoshi Nagano, Yasuhiro Ishidou, Setsuro Komiya
Organizer
Orthopaedic Research Society Annual Meeting 2016
Place of Presentation
Orlando, USA
Year and Date
2016-03-05
Related Report
Int'l Joint Research
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[Presentation] Myc Target 1 (MYCT1) Gene is a Novel Target of TGF-β Signaling to Promote Differentiation of Chondrosarcoma Cells2015
Author(s)
Yahiro, Y; Maeda, S; Shinohara, N; Matsuyama, K; Kawamura, I; Setoguchi, T; Nagano, S; Yokouchi, M; Ishidou, Y; Komiya, S
Organizer
Orthopedic Research Society 2015 Annual Meeting
Place of Presentation
アメリカ合衆国、ラスベガス (MGM Grand Hotel)
Year and Date
2015-03-28 – 2015-03-31
Related Report