| Project/Area Number |
26462312
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
Arai Yuji 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (50347449)
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| Co-Investigator(Kenkyū-buntansha) |
久保 俊一 京都府立医科大学, 医学(系)研究科(研究院), 教授 (20178031)
中川 周士 京都府立医科大学, 医学(系)研究科(研究院), 助教 (30643382)
土田 真嗣 京都府立医科大学, 医学(系)研究科(研究院), 助教 (10719834)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | HIF-1α / hyaluronic acid / CD44 / articular cartilage / extracellular matrix / hypoxia / 軟骨 / 低酸素 / ヒアルロン酸 / 変形性関節症 / 低酸素環境 / 細胞外マトリックス / 高分子ヒアルロン酸 / HSP70 |
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| Outline of Final Research Achievements |
High molecular weight hyaluronic acid (HA) is widely used clinically as an intra-articular injection for osteoarthritis, but the pharmacological effect under hypoxic condition is not clear. The purpose of this study is to clarify the effect of HA on articular cartilage under hypoxia. It was revealed that the cartilage assimilatory pharmacological action of the exogenous macromolecule HA on articular cartilage is promoted in hypoxic condition. As a mechanism, it was shown that the expression of CD44 was enhanced by the HIF-1α pathway and the binding affinity to the exogenous polymer HA was improved. Maintaining the hypoxic environment around chondrocytes is important for exerting the anabolic effect of exogenous high molecular weight HA.
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