| Project/Area Number |
26462317
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | Saitama Medical University |
|---|
Principal Investigator |
Mizuno Yosuke 埼玉医科大学, 医学部, 講師 (30406532)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
仲地 豊 埼玉医科大学, 医学部, 助教 (10522097)
|
|---|
| Project Period (FY) |
2014-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | 非コードRNA / 間葉系幹細胞 / 脂肪細胞分化 / 骨芽細胞分化 / マイクロRNA / RNA / トランスクリプトーム解析 / トランスクリプトーム / 転写開始点 / スプライシングバリアント / 選択的ポリA付加 |
|---|
| Outline of Final Research Achievements |
Mesenchymal stem cells can differentiate into various cell types. Particularly the regulatory mechanisms of the balance between differentiation to adipocytes and osteoblasts are closely linked to the pathogenesis of metabolic syndrome and osteoporosis. We performed detailed RNA expression analysis using differentiating cells. Differential expression of each exon and ncRNAs are comprehensively analyzed using exon array, miRNA array and RNA-sequencing. As a result, we detected various RNA variants of other genes and ncRNAs that are differentially expressed between both types of differentiation. We estimate that specific RNA variants and ncRNAs which regulate the differentiation are involved in those differentially expressed RNA variants, and currently the experiments for their validation are underway
|
