T cell and macrophage derived proinflammatory cytokines-induced joint destruction
| Project/Area Number |
26462321
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Toho University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
中川 晃一 東邦大学, 医学部, 教授 (30400823)
青木 保親 千葉大学, 大学院医学研究院, 特任教授 (70584001)
齊藤 雅彦 東邦大学, 医学部, 助教 (30718747)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 関節リウマチ / 滑膜 / 炎症性サイトカイン / TNF-α / IFN-γ / IL-17 |
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| Outline of Final Research Achievements |
In this study, mRNA expression levels of IFN-γ, IL-17 and TNF-α were investigated in individual patients with RA and the correlations between pairs of these three pro-inflammatory cytokines were analyzed. Synovial tissues were obtained during arthroplasties from 24 RA patients and qRT-PCR for IFN-γ, IL-17 and TNF-α was performed.
Based on relative expression levels of the three pro-inflammatory cytokines, patients were classified into 3 major types; an IFN-γ plus TNF-α-dominant type, an IL-17-dominant type, and the other type. TNF-α expression levels were correlated with IFN-γ. In addition, there was a negative correlation between TNF-α and IL-17, and IFN-γ and IL-17. Median relative expression levels of TNF-α have no significant difference between the TNFi and the csDMARDs groups. In the rheumatoid synovial tissues, expression levels of TNF-α were modulated in parallel with IFN-γ, and TNF-α and IL-17, or IFN-γ and IL-17 did not co-express at high levels.
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Report
(4 results)
Research Products
(11 results)
