Basic research for the establishment of one-step cartilage repair technique using silk fibroin sponge with synovial cells and minced cartilage
| Project/Area Number |
26462322
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Toho University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
中島 新 東邦大学, 医学部, 准教授 (60583995)
青木 保親 千葉大学, 大学院医学研究院, 特任教授 (70584001)
齊藤 雅彦 東邦大学, 医学部, 助教 (30718747)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 関節病学 / 組織工学 / 軟骨再生 / 滑膜 / フィブロイン / ヒト |
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| Outline of Final Research Achievements |
[Purpose] To examine if human synovial cells can be differentiate into chondrocytes on silk fibroin sponges (FS). [Material and methods] Human synovial cells were seeded on FS and cultured in the conditioned media for chondrocyte differentiation in the presence or absence of BMP-2. The effect of co-culture with minced cartilage was investigated histologically and biochemically. [Results] Synovial cells cultured with BMP-2 were shown to differentiate into chondrocytes on FS. Expression of SOX9 and ACAN gene and COL-2/COL-1 expression rate were elevated by co-culture with minced cartilage. [Discussion] The results of this study suggest that FS with synovial cells and minced cartilage could apply to articular cartilage repair.
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] Identification of genes required for the spontaneous repair of partial-thickness cartilage defects in immature rats.2016
Author(s)
Ikegawa N, Sasho T, Yamaguchi S, Saito M, Akagi R, Muramatsu Y, Akatsu Y, Fukawa T, Nakagawa K, Nakajima A, Suzuki T, Takahashi K.
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Journal Title
Connect Tissue Res
Volume: 31
Issue: 3
Pages: 190-199
DOI
Related Report
Peer Reviewed
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[Presentation] Covering large chondral defect with silk fibroin sponge after marrow stimulation enhances cartilage repair in a canine model2016
Author(s)
Nakagawa K, Saito M, Nakajima A, Sonobe M, Takahashi H, Taniguchi S, Yamada M, Wada Y, Tamada Y, Tomita N
Organizer
The 13th World Congress of the International Cartilage Repair Society (ICRS), Sorrento, Italy, 2016. 9. 24-27
Place of Presentation
Sorrento, Italy
Year and Date
2016-09-25
Related Report
Int'l Joint Research
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[Presentation] 広範囲関節軟骨欠損に対する骨髄刺激とフィブロイン被覆の併用療法の効果2014
Author(s)
齊藤雅彦, 中川晃一, 富田直秀, 玉田靖, 柴田孝史, 園部正人, 中島新, 高橋宏, 谷口慎治, 山田学, 平方栄一, 青木秀之, 齋藤知行
Organizer
第29回日本整形外科学会基礎学術集会
Place of Presentation
城山観光ホテル（鹿児島県鹿児島市）
Year and Date
2014-10-09 – 2014-10-10
Related Report
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