Effects of anesthetic-induced precoditioning on cardiac ion channels

• Project/Area Number: 26462326
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Anesthesiology
• Research Institution: Asahikawa Medical College
• Principal Investigator: TAMPO Akihito 旭川医科大学, 医学部, 講師 (80531524)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
• Keywords: プレコンディショニング / 心筋傷害 / 麻酔プレコンディショニング / 虚血・再灌流傷害 / 虚血再灌流障害 / ナトリウム電流 / イオンチャネル / 麻酔・蘇生学

Outline of Final Research Achievements

In this study, we evaluated the APC effects on sodium channel in the Dahl-S rats which were not pre-conditioned by anesthetics. Increase in the sodium current, and channel protein expression by APC was abolished in the Dahl-S rats. Five minutes oxidative stress and reperfusion injury increased the sodium current in 10 and 15 minutes reperfusion, but the change was not observed in the anesthetic-preconditioned cardiomyocytes. These results suggested that APC treatment reduced myocytes injury by sodium overload and following calcium overload. The increased sodium current by APC was decreased by 30 minutes ischemia-reperfusion injury.