Cross-talk regulation by platelets, coagulation system and fibrinolysis system in response to sepsis
| Project/Area Number |
26462335
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Anesthesiology
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| Research Institution | Gifu University |
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Principal Investigator |
akamatsu shigeru 岐阜大学, 大学院医学系研究科, 非常勤講師 (20167828)
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| Co-Investigator(Kenkyū-buntansha) |
小澤 修 岐阜大学, 大学院医学系研究科, 教授 (90225417)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 血小板 / 凝固因子 / 線溶系 / 血栓 / PDGF-AB / 可溶型 / CD40 ligand / HSP27 / 凝固系 / コラーゲン / スフィンゴシン1リン酸 / p38 MAP kinase / 活性化第X因子 / p44/p42 MAP kinase / thromboxane A2 / Rac / soluble CD40 ligand |
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| Outline of Final Research Achievements |
Selective inhibitors of factor Xa (FXa) are widely recognized as useful therapeutic tools for stroke prevention in non-valvular atrial fibrillation or venous thrombosis. Thrombin generated by the activation of factor X to FXa, acts as a potent activator of human platelets. However, little is known whether FXa inhibitors directly affect the function of human platelets. We previously demonstrated that collagen induces the phosphorylation of heat shock protein 27 (HSP27), a small-molecular weight HSP via p44/p42 mitogen-activated protein (MAP) kinase in human platelets, eventually resulting in the release of HSP27. In the present study, we investigated the direct effect of FXa inhibitors (edoxaban and rivaroxaban) on the collagen-induced human platelet activation. Our results strongly suggest that FXa inhibitor reduces the collagen-stimulated release of phosphorylated HSP27 from human platelets due to the inhibition of HSP27 phosphorylation via p44/p42 MAP kinase.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] AICAR reduces collagen-stimulated secretion of PDGF-AB and release of soluble CD40 ligand from human platelets: suppression of HSP27 phosphorylation via p44/p42 MAP kinase.2016
Author(s)
Tsujimoto M, Tokuda H, Kuroyanagi G, Yamamoto N, Kainuma S, Matsushima-Nishiwaki R, Onuma T, Iida Y, Kojima A, Sawada S, Doi T, Enomoto Y, Tanabe K, Akamatsu S, Iida H, Ogura S, Otsuka T, Kozawa O and Iwama T
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Journal Title
Exp Ther Med.
Volume: 12
Pages: 1107-1112
Related Report
Peer Reviewed / Open Access
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[Journal Article] Thrombin Receptor-Activating Protein (TRAP)-Activated Akt Is Involved in the Release of Phosphorylated-HSP27 (HSPB1) from Platelets in DM Patients.2016
Author(s)
Tokuda H, Kuroyanagi G, Tsujimoto M, Matsushima-Nishiwaki R, Akamatsu S, Enomoto Y, Iida H, Otsuka T, Ogura S, Iwama T, Kojima K, Kozawa O.
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Journal Title
Int J Mol Sci
Volume: 17
Issue: 5
Pages: 737-737
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Involvement of Rac in thromboxane A2-induced human platelet activation: Regulation of sCD40 ligand release and PDGF-AB secretion.2014
Author(s)
Kageyama Y, Doi T, Matsushima-Nishiwaki R, Iida Y, Akamatsu S, Kondo A, Kuroyanagi G, Yamamoto N, Mizutani J, Otsuka T, Tokuda H, Iida H, Kozawa O, Ogura S.
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Journal Title
Mol Med Rep.
Volume: 10(1)
Issue: 1
Pages: 107-12
DOI
Related Report
Peer Reviewed
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